dATP 100 mM PCR Grade

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Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
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Physical properties

Molecular weight	491.18
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

1927-31-7

Molecular weight

491.18

2′-Deoxyadenosine 5′-triphosphate sodium salt (dATP) solution is used in DNA synthesis reactions such as PCR, DNA sequencing and molecular cloning techniques. This dATP solution is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of dATP *100 mM PCR Grade* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	203.591 µL	1.018 mL	2.036 mL	10.18 mL	20.359 mL
5 mM	40.718 µL	203.591 µL	407.183 µL	2.036 mL	4.072 mL
10 mM	20.359 µL	101.796 µL	203.591 µL	1.018 mL	2.036 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Chemical structure for dATP *100 mM PCR Grade*.

References

View all 50 references: Citation Explorer

Enzymatic Synthesis of Chimeric DNA Oligonucleotides by in Vitro Transcription with dTTP, dCTP, dATP, and 2'-Fluoro Modified dGTP.
Authors: Kapustina, Žana and Jasponė, Aistė and Dubovskaja, Varvara and Mackevičius, Gytis and Lubys, Arvydas
Journal: ACS synthetic biology (2021): 1625-1632

Development of a Novel Bioluminescence Pyrophosphate Assay for the High-Sensitivity Detection of Hepatitis B Virus.
Authors: Tan, Guolei and Fei, Zhongjie and Wei, Rongbin and Wu, Xuping and Xiao, Pengfeng
Journal: Applied biochemistry and biotechnology (2021)

New bioluminescence pyrophosphate assay for high-sensitivity detection of food-borne pathogens.
Authors: Fei, Zhongjie and Zhou, Dongrui and Li, Na and Xiao, Pengfeng
Journal: Luminescence : the journal of biological and chemical luminescence (2020): 355-364

Plasmodium falciparum DDX55 is a nucleocytoplasmic protein and a 3'-5' direction-specific DNA helicase.
Authors: Yasmin, Rahena and Kaur, Inderjeet and Tuteja, Renu
Journal: Protoplasma (2020): 1049-1067

Polymerase synthesis of four-base DNA from two stable dimeric nucleotides.
Authors: Mohsen, Michael G and Ji, Debin and Kool, Eric T
Journal: Nucleic acids research (2019): 9495-9501

Duplex Electrochemical DNA Sensor to Detect Bacillus anthracis CAP and PAG DNA Targets Based on the Incorporation of Tailed Primers and Ferrocene-Labeled dATP.
Authors: Magriñá, Ivan and Jauset-Rubio, Miriam and Ortiz, Mayreli and Tomaso, Herbert and Simonova, Anna and Hocek, Michal and O'Sullivan, Ciara K
Journal: ACS omega (2019): 21900-21908

Electrochemical genosensor for the direct detection of tailed PCR amplicons incorporating ferrocene labelled dATP.
Authors: Magriñá, Ivan and Toldrà, Anna and Campàs, Mònica and Ortiz, Mayreli and Simonova, Anna and Katakis, Ioanis and Hocek, Michal and O'Sullivan, Ciara K
Journal: Biosensors & bioelectronics (2019): 76-82

Self-Priming Enzymatic Fabrication of Multiply Modified DNA.
Authors: Whitfield, Colette J and Little, Rachel C and Khan, Kasid and Ijiro, Kuniharu and Connolly, Bernard A and Tuite, Eimer M and Pike, Andrew R
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 15267-15274

GATA4 Is Sufficient to Establish Jejunal Versus Ileal Identity in the Small Intestine.
Authors: Thompson, Cayla A and Wojta, Kevin and Pulakanti, Kirthi and Rao, Sridhar and Dawson, Paul and Battle, Michele A
Journal: Cellular and molecular gastroenterology and hepatology (2017): 422-446

Alr2954 of Anabaena sp. PCC 7120 with ADP-ribose pyrophosphatase activity bestows abiotic stress tolerance in Escherichia coli.
Authors: Singh, Prashant Kumar and Shrivastava, Alok Kumar and Singh, Shilpi and Rai, Ruchi and Chatterjee, Antra and Rai, L C
Journal: Functional & integrative genomics (2017): 39-52