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DBCO-18-dUTP [5-DBCO-PEG4-dUTP]
Product key features
- DBCO-18-dUTP is used to introduce DBCO into a DNA
- Highly efficient
- A versatile of biological tags can be introduced
Product description
DBCO-18-dUTP, also called 5-DBCO-PEG4-dUTP, is used for two-step labeling of DNA/cDNA, e.g. by PCR. It is enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulted DBCO-functionalized DNA/cDNA can subsequently be labeled via Cu-free click chemistry that is also known as strain-promoted azide-alkyne cycloaddition (SPAAC) with a desired tag such as biotin, a fluorophore or a biopolymer, e.g., an enzyme or antibody.
References
View all 3 references: Citation Explorer
Synthesis and Direct Observation of Thermoresponsive DNA Copolymers.
Authors: Li, Songsong and Schroeder, Charles M
Journal: ACS macro letters (2018): 281-286
Authors: Li, Songsong and Schroeder, Charles M
Journal: ACS macro letters (2018): 281-286
Azide and trans-cyclooctene dUTPs: incorporation into DNA probes and fluorescent click-labelling.
Authors: Ren, Xiaomei and El-Sagheer, Afaf H and Brown, Tom
Journal: The Analyst (2015): 2671-8
Authors: Ren, Xiaomei and El-Sagheer, Afaf H and Brown, Tom
Journal: The Analyst (2015): 2671-8
Enzymatic incorporation and fluorescent labelling of cyclooctyne-modified deoxyuridine triphosphates in DNA.
Authors: Ren, Xiaomei and Gerowska, Marta and El-Sagheer, Afaf H and Brown, Tom
Journal: Bioorganic & medicinal chemistry (2014): 4384-90
Authors: Ren, Xiaomei and Gerowska, Marta and El-Sagheer, Afaf H and Brown, Tom
Journal: Bioorganic & medicinal chemistry (2014): 4384-90
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