DBCO-Cy5

This azadibenzocyclooctyne-cyanine dye derivative is a versatile labeling reagent for detection of azide containing molecules or compounds. Cyclooctynes are useful in strain-promoted copper-free azide-alkyne cycloaddition reactions. This dibenzocyclooctyne will react with azide-functionalized compounds or biomolecules without the need for a Cu(I) catalyst to result in a stable triazole linkage. AAT Bioquest offer a broad range of dye azide compounds (such as coumarin azides, fluorescein azides, rhodamine azides and cyanine azides) for click reactions.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DBCO-Cy5 to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	82.535 µL	412.677 µL	825.355 µL	4.127 mL	8.254 mL
5 mM	16.507 µL	82.535 µL	165.071 µL	825.355 µL	1.651 mL
10 mM	8.254 µL	41.268 µL	82.535 µL	412.677 µL	825.355 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (260 nm)	Correction Factor (280 nm)	Correction Factor (482 nm)	Correction Factor (565 nm)
Cy3B DBCO	560	571	120000¹	0.58¹	0.048	0.069	-	-
Cy5 aldehyde	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cy5 tertrazine [Cy5 tertrazine]	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
Cy5 tetrazine	651	670	250000¹	0.27¹, 0.4²	0.02	0.03	0.009	0.09
DBCO-Cy3	555	569	150000¹	0.15¹	0.07	0.073	-	-

Citations

View all 3 citations: Citation Explorer

Bioorthogonal SERS-Bioluminescence Dual-Modal Imaging for Real-Time Tracking of Triple-Negative Breast Cancer Metastasis
Authors: Zhang, Wei and Wang, Sisi and Xing, Yanlong and Luo, Xianzhu and Wang, Rui and Yu, Fabiao
Journal: Acta Biomaterialia (2025)

Synthesis, characterization, and biological activity of spherical nucleic acid (SNA) constructs for cancer therapy and imaging
Authors: Calabrese, Colin
Journal: (2017)

Site-specific chemical conjugation of human Fas ligand extracellular domain using trans-cyclooctene--methyltetrazine reactions
Authors: Muraki, Michiro and Hirota, Kiyonori
Journal: BMC biotechnology (2017): 56

References

View all 49 references: Citation Explorer

Beyond click chemistry - supramolecular interactions of 1,2,3-triazoles
Authors: Schulze B, Schubert US.
Journal: Chem Soc Rev (2014): 2522

Calixarene-based chemosensors by means of click chemistry
Authors: Song M, Sun Z, Han C, Tian D, Li H, Kim JS.
Journal: Chem Asian J (2014): 2344

Use of click-chemistry in the development of peptidomimetic enzyme inhibitors
Authors: Fabbrizzi P, Menchi G, Guarna A, Trabocchi A.
Journal: Curr Med Chem (2014): 1467

Applications of copper-catalyzed click chemistry in activity-based protein profiling
Authors: Martell J, Weerapana E.
Journal: Molecules (2014): 1378

'Click chemistry' for diagnosis: a patent review on exploitation of its emerging trends
Authors: M, undefined and hare A, Banerjee P, Bhutkar S, Hirwani R.
Journal: Expert Opin Ther Pat (2014): 1287

Page updated on May 19, 2025

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Catalog Number	923
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Physical properties

Molecular weight	1211.60
Solvent	DMSO

Spectral properties

Correction Factor (260 nm)	0.02
Correction Factor (280 nm)	0.03
Correction Factor (482 nm)	0.009
Correction Factor (565 nm)	0.09
Extinction coefficient (cm ^-1 M ^-1)	250000¹
Excitation (nm)	651
Emission (nm)	670
Quantum yield	0.27¹, 0.4²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501