DBCO-PEG4-NHS Ester

Cu-free click reaction, i.e., the strain-promoted alkyne-azide cycloaddition (SPAAC) is a bioorthogonal reaction utilizing a pair of reagents, cyclooctynes and azides that exclusively and efficiently react with each other while remain inert to naturally occurring functional groups such as hydroxy, amino and thiol groups. SPAAC enables labeling a wide variety of biomolecules without any auxiliary reagents in an aqueous and otherwise complex chemical environment through the formation of a stable triazole. Among the large number of known cyclooctynes, dibenzocyclooctyne (DBCO) compounds comprise a class of reagents that possesses reasonably fast kinetics and good stability in aqueous buffers. Within physiological temperature and pH ranges, the DBCO group will not react with thiol, amino or hydroxy groups that are naturally present in many biomolecules. DBCO-PEG4-NHS Ester is one of the most common reagents used for introducing DBCO group into a biomolecule via the modification of the amino groups.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
4530	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DBCO-PEG4-NHS Ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	153.917 µL	769.586 µL	1.539 mL	7.696 mL	15.392 mL
5 mM	30.783 µL	153.917 µL	307.834 µL	1.539 mL	3.078 mL
10 mM	15.392 µL	76.959 µL	153.917 µL	769.586 µL	1.539 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	649.7 g/mol	=	mL	x	mM	=	mmol

Citations

View all 16 citations: Citation Explorer

The one-pot nonhydrolysis Staudinger reaction and Staudinger or SPAAC ligation

Authors:

Cheng, L., Kang, X., Wang, D., Gao, Y., Yi, L., Xi, Z.

Journal:

Org Biomol Chem (2019): 5675-5679

Highly reactive bis-cyclooctyne-modified diarylethene for SPAAC-mediated cross-linking

Authors:

Strizhak, A. V., Sharma, K., Babii, O., Afonin, S., Ulrich, A. S., Komarov, I. V., Spring, D. R.

Journal:

Org Biomol Chem (2018): 8559-8564

Optimization of Chemoenzymatic Mass Tagging by Strain-Promoted Cycloaddition (SPAAC) for the Determination of O-GlcNAc Stoichiometry by Western Blotting

Authors:

Darabedian, N., Thompson, J. W., Chuh, K. N., Hsieh-Wilson, L. C., Pratt, M. R.

Journal:

Biochemistry (2018): 5769-5774

Reproducible Dendronized PEG Hydrogels via SPAAC Cross-Linking

Authors:

Hodgson, S. M., McNelles, S. A., Abdullahu, L., Marozas, I. A., Anseth, K. S., Adronov, A.

Journal:

Biomacromolecules (2017): 4054-4059

Fused Bicyclic Caspase-1 Inhibitors Assembled by Copper-Free Strain-Promoted Alkyne-Azide Cycloaddition (SPAAC)

Authors:

Qian, L., Zhang, C. J., Wu, J., Yao, S. Q.

Journal:

Chemistry (2017): 360-369

Physical properties

Molecular weight	649.70
Solvent	DMSO

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	1353016-71-3

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Page updated on October 13, 2025