Name: DCFH-DA [2',7'-Dichlorodihydrofluorescein diacetate] *CAS 4091-99-0*
Brand: AAT Bioquest
SKU: 15204
Price: 90 USD
Availability: InStock

DCFH-DA

2',7'-Dichlorodihydrofluorescein diacetate; CAS 4091-99-0

DCFH-DA, 2',7'-Dichlorodihydrofluorescein diacetate (also called 2',7'-dichlorofluorescin diacetate), is hydrolyzed by cellular esterases to 2',7'-dichlorodihydrofluorescein (also called 2',7'-dichlorofluorescin) and is then oxidized to 2',7'-dichlorofluorescein primarily by H2O2. 2',7'-dichlorodihydrofluorescein diacetate might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. This probe is widely used to monitoring cellular redox processes.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
15204	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DCFH-DA [2',7'-Dichlorodihydrofluorescein diacetate] *CAS 4091-99-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	205.217 µL	1.026 mL	2.052 mL	10.261 mL	20.522 mL
5 mM	41.043 µL	205.217 µL	410.433 µL	2.052 mL	4.104 mL
10 mM	20.522 µL	102.608 µL	205.217 µL	1.026 mL	2.052 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	487.29 g/mol	=	mL	x	mM	=	mmol

Citations

View all 22 citations: Citation Explorer

Tetrahydrocurcumin Protects Microglial Cells Against Pseudomonas aeruginosa Lipopolysaccharide-Induced Reactive Oxygen Species Production and Cathepsin B to Activate NLRP3 Inflammasome-Mediated Pyroptosis Via the HO-1 and p38/JNK Pathway

Authors:

Lin, Hui-Wen and Chen, Tzu-Chun and Wang, Inga and Yeh, Jui-Hsuan and Tsou, Shang-Chun and Sun, Lee and Chung, Shih-Chi and Chuang, Chen-Ju and Chang, Yuan-Yen

Journal:

International Journal of Medical Sciences (2025): 4049--4062

Hemocyanin controls Vibrio levels in hemolymph by modulating intracellular ROS via p38 MAPK

Authors:

Cheng, Jiawei and Shi, Yunyu and Kong, Yonghui and Wu, Yuxi and Wang, Yankang and Aweya, Jude Juventus and Bao, Shiyuan and Nie, Junjie and Feng, Qian and Zhao, Yongzhen and others,

Journal:

Virulence (2025): 2546066

Safe transportation and targeted destruction: Albumin encapsulated aggregation-induced emission photosensitizer nanoaggregate for tumor photodynamic therapy through mitochondria damage-triggered pyroptosis

Authors:

Cao, Juanmei and Qu, Yong and Zhu, Shaojie and Zhan, Jinshan and Xu, Yiting and Jin, Yifan and Wang, Yuqing and Li, Zhuoxia and Chai, Chuxing and Wu, Xiangwei and others,

Journal:

Aggregate (2024): e637

A color morph-specific salivary carotenoid desaturase enhances plant photosynthesis and facilitates phloem feeding of Myzus persicae (Sulzer)

Authors:

Ge, Panpan and Guo, Huijuan and Li, Danyang and Zhu-Salzman, Keyan and Sun, Yucheng

Journal:

Pest Management Science (2024)

Reinforced Immunogenic Endoplasmic Reticulum Stress and Oxidative Stress via an Orchestrated Nanophotoinducer to Boost Cancer Photoimmunotherapy

Authors:

Yang, Zhenzhen and Teng, Yulu and Lin, Meng and Peng, Yiwei and Du, Yitian and Sun, Qi and Gao, Datong and Yuan, Quan and Zhou, Yu and Yang, Yiliang and others,

Journal:

ACS nano (2024)

References

View all 11 references: Citation Explorer

Kinetic analysis of fluorescein and dihydrofluorescein effluxes in tumour cells expressing the multidrug resistance protein, MRP1

Authors:

Saengkhae C, Loetchutinat C, Garnier-Suillerot A.

Journal:

Biochem Pharmacol (2003): 969

Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes

Authors:

Diaz G, Liu S, Isola R, Diana A, Falchi AM.

Journal:

Histochem Cell Biol (2003): 319

Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123

Authors:

Hempel SL, Buettner GR, O'Malley YQ, Wessels DA, Flaherty DM.

Journal:

Free Radic Biol Med (1999): 146

A rapid diagnostic test for the viability of early cattle and rabbit embryos using diacetyl-fluorescin

Authors:

Schilling E, Dopke HH.

Journal:

Naturwissenschaften (1978): 658

Determination of fluorescin sodium in ophthalmic solutions

Authors:

Robertson EJ, Patel JA.

Journal:

Am J Hosp Pharm (1968): 598

Physical properties

Molecular weight	487.29
Solvent	DMSO

Spectral properties

Excitation (nm)	505
Emission (nm)	526

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	4091-99-0

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	FITC filter set

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Page updated on October 6, 2025