dCTP 100 mM PCR Grade

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Physical properties

Molecular weight	467.16
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

CAS

2056-98-6

Molecular weight

467.16

2'-Deoxycytidine-5'-Triphosphate (dCTP) solution is used in DNA synthesis reactions such as PCR, DNA sequencing and molecular cloning techniques. This dATP solution is suitable for many applications where high-quality reagents are required. Such procedures include reverse transcription (RT), polymerase chain reaction (PCR), RT-PCR, DNA labeling reactions, and sequencing/cycle sequencing analysis.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of dCTP *100 mM PCR Grade* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	214.059 µL	1.07 mL	2.141 mL	10.703 mL	21.406 mL
5 mM	42.812 µL	214.059 µL	428.119 µL	2.141 mL	4.281 mL
10 mM	21.406 µL	107.03 µL	214.059 µL	1.07 mL	2.141 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Chemical structure for dCTP *100 mM PCR Grade*.

References

View all 50 references: Citation Explorer

Enzymatic Synthesis of Chimeric DNA Oligonucleotides by in Vitro Transcription with dTTP, dCTP, dATP, and 2'-Fluoro Modified dGTP.
Authors: Kapustina, Žana and Jasponė, Aistė and Dubovskaja, Varvara and Mackevičius, Gytis and Lubys, Arvydas
Journal: ACS synthetic biology (2021): 1625-1632

Polymerase synthesis of four-base DNA from two stable dimeric nucleotides.
Authors: Mohsen, Michael G and Ji, Debin and Kool, Eric T
Journal: Nucleic acids research (2019): 9495-9501

Self-Priming Enzymatic Fabrication of Multiply Modified DNA.
Authors: Whitfield, Colette J and Little, Rachel C and Khan, Kasid and Ijiro, Kuniharu and Connolly, Bernard A and Tuite, Eimer M and Pike, Andrew R
Journal: Chemistry (Weinheim an der Bergstrasse, Germany) (2018): 15267-15274

PCR amplification of GC-rich DNA regions using the nucleotide analog N4-methyl-2'-deoxycytidine 5'-triphosphate.
Authors: Flores-Juárez, Cyntia R and González-Jasso, Eva and Antaramian, Anaid and Pless, Reynaldo C
Journal: BioTechniques (2016): 175-182

Flexible double-headed cytosine-linked 2'-deoxycytidine nucleotides. Synthesis, polymerase incorporation to DNA and interaction with DNA methyltransferases.
Authors: Kielkowski, Pavel and Cahová, Hana and Pohl, Radek and Hocek, Michal
Journal: Bioorganic & medicinal chemistry (2016): 1268-76

Molecular identification of Anisakis and Hysterothylacium larvae in marine fishes from the East China Sea and the Pacific coast of central Japan.
Authors: Kong, Qingming and Fan, Lanfen and Zhang, Junhe and Akao, Nobuaki and Dong, Kewei and Lou, Di and Ding, Jianzu and Tong, Qunbo and Zheng, Bin and Chen, Rui and Ohta, Nobuo and Lu, Shaohong
Journal: International journal of food microbiology (2015): 1-7

Hi-C in Budding Yeast.
Authors: Belton, Jon-Matthew and Dekker, Job
Journal: Cold Spring Harbor protocols (2015): 649-61

Fluorescence polarization-based method with bisulfite conversion-specific one-label extension for quantification of single CpG dinucleotide methylation.
Authors: Li, Shufen and Wang, Zhongju and Zhou, Lin and Luo, Fu and Zhao, Cunyou
Journal: Genome (2015): 357-63

Transcriptome analysis of Capsicum annuum varieties Mandarin and Blackcluster: assembly, annotation and molecular marker discovery.
Authors: Ahn, Yul-Kyun and Tripathi, Swati and Kim, Jeong-Ho and Cho, Young-Il and Lee, Hye-Eun and Kim, Do-Sun and Woo, Jong-Gyu and Cho, Myeong-Cheoul
Journal: Gene (2014): 494-9

LNA-modified isothermal oligonucleotide microarray for differentiating bacilli of similar origin.
Authors: Yan, Jing and Yuan, Ying and Mu, Runqing and Shang, Hong and Guan, Yifu
Journal: Journal of biosciences (2014): 795-804