ddATP [2',3'-Dideoxyadenosine-5'-triphosphate] 10 mM in ddH2O

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Telephone	1-800-990-8053
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Physical properties

Molecular weight	475.18
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

475.18

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase. It was developed by Frederick Sanger and colleagues in 1977. Although the newer NGS technologies are becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample, Sanger sequencing with 99.99% accuracy is still the “gold standard” for clinical research sequencing. dd-ATP is one of the four critical ddNTP components for performing Sanger sequencing.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of ddATP [2',3'-Dideoxyadenosine-5'-triphosphate] *10 mM in ddH2O* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	210.447 µL	1.052 mL	2.104 mL	10.522 mL	21.045 mL
5 mM	42.089 µL	210.447 µL	420.893 µL	2.104 mL	4.209 mL
10 mM	21.045 µL	105.223 µL	210.447 µL	1.052 mL	2.104 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Chemical structure for ddATP [2',3'-Dideoxyadenosine-5'-triphosphate].

References

View all 4 references: Citation Explorer

Denaturation fingerprinting: two related mutation detection methods especially advantageous for high G + C regions.
Authors: Liu, Q and Weinshenker, B G and Wingerchuk, D M and Sommer, S S
Journal: BioTechniques (1998): 140-7

Slow rate of phosphodiester bond formation accounts for the strong bias that Taq DNA polymerase shows against 2',3'-dideoxynucleotide terminators.
Authors: Brandis, J W and Edwards, S G and Johnson, K A
Journal: Biochemistry (1996): 2189-200

Recombinant human hepatitis B virus reverse transcriptase is active in the absence of the nucleocapsid or the viral replication origin, DR1.
Authors: Seifer, M and Standring, D N
Journal: Journal of virology (1993): 4513-20

The enzymatic termination of polydeoxynucleotides by 2',3'-dideoxyadenosine triphosphate.
Authors: Toji, L and Cohen, S S
Journal: Proceedings of the National Academy of Sciences of the United States of America (1969): 871-7