ddATP [2',3'-Dideoxyadenosine-5'-triphosphate] *10 mM in ddH2O*

ddATP

2',3'-Dideoxyadenosine-5'-triphosphate; 10 mM in ddH2O

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase. It was developed by Frederick Sanger and colleagues in 1977. Although the newer NGS technologies are becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample, Sanger sequencing with 99.99% accuracy is still the “gold standard” for clinical research sequencing. dd-ATP is one of the four critical ddNTP components for performing Sanger sequencing.

Chemical structure for ddATP [2',3'-Dideoxyadenosine-5'-triphosphate].

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17209	1 umole	Price

References

View all 4 references: Citation Explorer

Denaturation fingerprinting: two related mutation detection methods especially advantageous for high G + C regions.

Authors:

Liu, Q and Weinshenker, B G and Wingerchuk, D M and Sommer, S S

Journal:

BioTechniques (1998): 140-7

Slow rate of phosphodiester bond formation accounts for the strong bias that Taq DNA polymerase shows against 2',3'-dideoxynucleotide terminators.

Authors:

Brandis, J W and Edwards, S G and Johnson, K A

Journal:

Biochemistry (1996): 2189-200

Recombinant human hepatitis B virus reverse transcriptase is active in the absence of the nucleocapsid or the viral replication origin, DR1.

Authors:

Seifer, M and Standring, D N

Journal:

Journal of virology (1993): 4513-20

The enzymatic termination of polydeoxynucleotides by 2',3'-dideoxyadenosine triphosphate.

Authors:

Toji, L and Cohen, S S

Journal:

Proceedings of the National Academy of Sciences of the United States of America (1969): 871-7

Physical properties

Molecular weight	475.18
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Telephone
Fax
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Technical Support	Contact us
Request quotation	Request
Purchase order	Send to sales@aatbio.com
Shipping	Standard overnight for United States, inquire for international

Page updated on October 4, 2025