ddCTP [2',3'-Dideoxycytidine-5'-triphosphate] *10 mM in ddH2O*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 451.16 |
Solvent | Water |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Dideoxynucleotides (ddNTPs), Nucleic Acid Building Blocks, RNA Purification & Analysis, Sanger Sequencing
Molecular weight 451.16 |
Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase. It was developed by Frederick Sanger and colleagues in 1977. Although the newer NGS technologies are becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample, Sanger sequencing with 99.99% accuracy is still the “gold standard” for clinical research sequencing. dd-CTP is one of the four critical ddNTP components for performing Sanger sequencing.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of ddCTP [2',3'-Dideoxycytidine-5'-triphosphate] *10 mM in ddH2O* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 221.651 µL | 1.108 mL | 2.217 mL | 11.083 mL | 22.165 mL |
5 mM | 44.33 µL | 221.651 µL | 443.302 µL | 2.217 mL | 4.433 mL |
10 mM | 22.165 µL | 110.825 µL | 221.651 µL | 1.108 mL | 2.217 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
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References
View all 5 references: Citation Explorer
Involvement of DNA polymerase beta in DNA replication and mutagenic consequences.
Authors: Servant, Laurence and Bieth, Anne and Hayakawa, Hiroshi and Cazaux, Christophe and Hoffmann, Jean-Sébastien
Journal: Journal of molecular biology (2002): 1039-47
Authors: Servant, Laurence and Bieth, Anne and Hayakawa, Hiroshi and Cazaux, Christophe and Hoffmann, Jean-Sébastien
Journal: Journal of molecular biology (2002): 1039-47
Fluorescence-based DHPLC for allelic quantification by single-nucleotide primer extension.
Authors: Kosaki, K and Yoshihashi, H and Ohashi, Y and Kosaki, R and Suzuki, T and Matsuo, N
Journal: Journal of biochemical and biophysical methods (2001): 111-9
Authors: Kosaki, K and Yoshihashi, H and Ohashi, Y and Kosaki, R and Suzuki, T and Matsuo, N
Journal: Journal of biochemical and biophysical methods (2001): 111-9
Denaturation fingerprinting: two related mutation detection methods especially advantageous for high G + C regions.
Authors: Liu, Q and Weinshenker, B G and Wingerchuk, D M and Sommer, S S
Journal: BioTechniques (1998): 140-7
Authors: Liu, Q and Weinshenker, B G and Wingerchuk, D M and Sommer, S S
Journal: BioTechniques (1998): 140-7
Synergistic inhibition of HIV-1 reverse transcriptase by combinations of chain-terminating nucleotides.
Authors: Villahermosa, M L and Martinez-Irujo, J J and Cabodevilla, F and Santiago, E
Journal: Biochemistry (1997): 13223-31
Authors: Villahermosa, M L and Martinez-Irujo, J J and Cabodevilla, F and Santiago, E
Journal: Biochemistry (1997): 13223-31
A fidelity assay using "dideoxy" DNA sequencing: a measurement of sequence dependence and frequency of forming 5-bromouracil X guanine base mispairs.
Authors: Lasken, R S and Goodman, M F
Journal: Proceedings of the National Academy of Sciences of the United States of America (1985): 1301-5
Authors: Lasken, R S and Goodman, M F
Journal: Proceedings of the National Academy of Sciences of the United States of America (1985): 1301-5
Application notes
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Are there any alternatives for ethidium bromide in agarose gels?
Are there any alternatives to Cy5?