ddGTP [2',3'-Dideoxyguanosine-5'-triphosphate] 10 mM in ddH2O

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Physical properties

Molecular weight	491.18
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

491.18

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase. It was developed by Frederick Sanger and colleagues in 1977. Although the newer NGS technologies are becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample, Sanger sequencing with 99.99% accuracy is still the “gold standard” for clinical research sequencing. dd-GTP is one of the four critical ddNTP components for performing Sanger sequencing.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of ddGTP [2',3'-Dideoxyguanosine-5'-triphosphate] *10 mM in ddH2O* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	203.591 µL	1.018 mL	2.036 mL	10.18 mL	20.359 mL
5 mM	40.718 µL	203.591 µL	407.183 µL	2.036 mL	4.072 mL
10 mM	20.359 µL	101.796 µL	203.591 µL	1.018 mL	2.036 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Chemical structure for ddGTP [2',3'-Dideoxyguanosine-5'-triphosphate].

References

View all 4 references: Citation Explorer

Detection and genotyping of human papillomavirus DNA in cervical cancer tissues with fluorescence polarization.
Authors: Gao, Yan-E and Zhang, Ju and Wu, Jing and Chen, Zhong-Can and Yan, Xiao-Jun
Journal: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica (2003): 1029-34

Microcapillary reactors using solid-phase DNA sequencing for direct sample introduction into slab gels.
Authors: Xu, Y and Bruch, R C and Soper, S A
Journal: BioTechniques (2000): 904-8, 910, 912

Isolation and characterization of a dideoxyguanosine triphosphate-resistant mutant of human immunodeficiency virus reverse transcriptase.
Authors: Prasad, V R and Lowy, I and de los Santos, T and Chiang, L and Goff, S P
Journal: Proceedings of the National Academy of Sciences of the United States of America (1991): 11363-7

Herpes simplex virus type 1 and human DNA polymerase interactions with 2'-deoxyguanosine 5'-triphosphate analogues. Kinetics of incorporation into DNA and induction of inhibition.
Authors: Reardon, J E
Journal: The Journal of biological chemistry (1989): 19039-44