ddTTP [2',3'-Dideoxythymidine-5'-triphosphate] 10 mM in ddH2O

Ordering information

Price
Catalog Number
Unit Size
Quantity

Add to cart

Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	466.17
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

466.17

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase. It was developed by Frederick Sanger and colleagues in 1977. Although the newer NGS technologies are becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample, Sanger sequencing with 99.99% accuracy is still the “gold standard” for clinical research sequencing. dd-TTP is one of the four critical ddNTP components for performing Sanger sequencing.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of ddTTP [2',3'-Dideoxythymidine-5'-triphosphate] *10 mM in ddH2O* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	214.514 µL	1.073 mL	2.145 mL	10.726 mL	21.451 mL
5 mM	42.903 µL	214.514 µL	429.028 µL	2.145 mL	4.29 mL
10 mM	21.451 µL	107.257 µL	214.514 µL	1.073 mL	2.145 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Images

Figure 1. Chemical structure for ddTTP [2',3'-Dideoxythymidine-5'-triphosphate].

References

View all 10 references: Citation Explorer

Strong positional preference in the interaction of LNA oligonucleotides with DNA polymerase and proofreading exonuclease activities: implications for genotyping assays.
Authors: Di Giusto, Daniel A and King, Garry C
Journal: Nucleic acids research (2004): e32

Detection and genotyping of human papillomavirus DNA in cervical cancer tissues with fluorescence polarization.
Authors: Gao, Yan-E and Zhang, Ju and Wu, Jing and Chen, Zhong-Can and Yan, Xiao-Jun
Journal: Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica (2003): 1029-34

Dye structure affects Taq DNA polymerase terminator selectivity.
Authors: Brandis, J W
Journal: Nucleic acids research (1999): 1912-8

Synergistic inhibition of HIV-1 reverse transcriptase by combinations of chain-terminating nucleotides.
Authors: Villahermosa, M L and Martinez-Irujo, J J and Cabodevilla, F and Santiago, E
Journal: Biochemistry (1997): 13223-31

Characterization of the p68/p58 heterodimer of human immunodeficiency virus type 2 reverse transcriptase.
Authors: Fan, N and Rank, K B and Poppe, S M and Tarpley, W G and Sharma, S K
Journal: Biochemistry (1996): 1911-7

Mechanism of inhibition of DNA synthesis by 1-beta-D-arabinofuranosyl-E-5-(2-bromovinyl)uracil.
Authors: Suzutani, T and Machida, H and Honess, R W
Journal: Microbiology and immunology (1993): 511-3

Wheat embryo DNA polymerase A reverse transcribes natural and synthetic RNA templates. Biochemical characterization and comparison with animal DNA polymerase gamma and retroviral reverse transcriptase.
Authors: Laquel, P and Sallafranque-Andreola, M and Tarrago-Litvak, L and Castroviejo, M and Litvak, S
Journal: Biochimica et biophysica acta (1990): 139-48

Expression of enzymatically active reverse transcriptase of simian immunodeficiency virus in bacteria: sensitivity to nucleotide analogue inhibitors.
Authors: Prasad, V R and Myrick, K and Haseltine, W and Goff, S P
Journal: Virology (1990): 896-900

3'-Deoxy-3'-fluorothymidinetriphosphate: inhibitor and terminator of DNA synthesis catalysed by DNA polymerase beta, terminal deoxynucleotidyl transferase and DNA polymerase I.
Authors: Matthes, E and Lehmann, C and Drescher, B and Büttner, W and Langen, P
Journal: Biomedica biochimica acta (1985): K63-73

A fidelity assay using "dideoxy" DNA sequencing: a measurement of sequence dependence and frequency of forming 5-bromouracil X guanine base mispairs.
Authors: Lasken, R S and Goodman, M F
Journal: Proceedings of the National Academy of Sciences of the United States of America (1985): 1301-5