Name: ddTTP [2',3'-Dideoxythymidine-5'-triphosphate] *10 mM in ddH2O*
Brand: AAT Bioquest
SKU: 17208
Price: 221 USD
Availability: InStock

ddTTP

2',3'-Dideoxythymidine-5'-triphosphate; 10 mM in ddH2O

Sanger sequencing, also known as the chain termination method, is a technique for DNA sequencing based upon the selective incorporation of chain-terminating dideoxynucleotides (ddNTPs) by DNA polymerase. It was developed by Frederick Sanger and colleagues in 1977. Although the newer NGS technologies are becoming common in clinical research labs due to their higher throughput capabilities and lower costs per sample, Sanger sequencing with 99.99% accuracy is still the “gold standard” for clinical research sequencing. dd-TTP is one of the four critical ddNTP components for performing Sanger sequencing.

Chemical structure for ddTTP [2',3'-Dideoxythymidine-5'-triphosphate].

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17208	1 umole	Price

References

View all 10 references: Citation Explorer

Strong positional preference in the interaction of LNA oligonucleotides with DNA polymerase and proofreading exonuclease activities: implications for genotyping assays.

Authors:

Di Giusto, Daniel A and King, Garry C

Journal:

Nucleic acids research (2004): e32

Detection and genotyping of human papillomavirus DNA in cervical cancer tissues with fluorescence polarization.

Authors:

Gao, Yan-E and Zhang, Ju and Wu, Jing and Chen, Zhong-Can and Yan, Xiao-Jun

Journal:

Sheng wu hua xue yu sheng wu wu li xue bao Acta biochimica et biophysica Sinica (2003): 1029-34

Dye structure affects Taq DNA polymerase terminator selectivity.

Authors:

Brandis, J W

Journal:

Nucleic acids research (1999): 1912-8

Synergistic inhibition of HIV-1 reverse transcriptase by combinations of chain-terminating nucleotides.

Authors:

Villahermosa, M L and Martinez-Irujo, J J and Cabodevilla, F and Santiago, E

Journal:

Biochemistry (1997): 13223-31

Characterization of the p68/p58 heterodimer of human immunodeficiency virus type 2 reverse transcriptase.

Authors:

Fan, N and Rank, K B and Poppe, S M and Tarpley, W G and Sharma, S K

Journal:

Biochemistry (1996): 1911-7

Physical properties

Molecular weight	466.17
Solvent	Water

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on October 8, 2025