logo
AAT Bioquest

DEAC,SE [7-Diethylaminocoumarin-3-carboxylic acid, succinimidyl ester] *CAS 139346-57-9*

Product Image
Product Image
Gallery Image 1
Ordering information
Price
Catalog Number
Unit Size
Quantity
Add to cart
Additional ordering information
Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
ShippingStandard overnight for United States, inquire for international
Request quotation
Physical properties
Molecular weight358.35
SolventDMSO
Spectral properties
Correction Factor (260 nm)0.14
Correction Factor (280 nm)0.12
Excitation (nm)411
Emission (nm)472
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


CAS
139346-57-9
Molecular weight
358.35
Correction Factor (260 nm)
0.14
Correction Factor (280 nm)
0.12
Excitation (nm)
411
Emission (nm)
472
The amine-reactive 7-diethylaminocoumarin-3-carboxylic acid, succinimidyl ester can be used to create blue-fluorescent bioconjugates. It has quite strong blue fluorescence. When compared with AMCA conjugates, conjugates of the UV-light-excitable 7-dialkylaminocoumarin fluorophore have much longer-wavelength excitation and slightly longer-wavelength emission spectra (~470 nm). This fluorescent dye is quite hydrophobic, and might be used for labeling live cells.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DEAC,SE [7-Diethylaminocoumarin-3-carboxylic acid, succinimidyl ester] *CAS 139346-57-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM279.057 µL1.395 mL2.791 mL13.953 mL27.906 mL
5 mM55.811 µL279.057 µL558.114 µL2.791 mL5.581 mL
10 mM27.906 µL139.528 µL279.057 µL1.395 mL2.791 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum


Open in Advanced Spectrum Viewer
spectrum

Spectral properties

Correction Factor (260 nm)0.14
Correction Factor (280 nm)0.12
Excitation (nm)411
Emission (nm)472

Images


References


View all 4 references: Citation Explorer
Fluorescent agonists for the Torpedo nicotinic acetylcholine receptor
Authors: Krieger F, Mourot A, Araoz R, Kotzyba-Hibert F, Molgo J, Bamberg E, Goeldner M.
Journal: Chembiochem (2008): 1146
A series of related nucleotide analogues that aids optimization of fluorescence signals in probing the mechanism of P-loop ATPases, such as actomyosin
Authors: Webb MR, Reid GP, Munasinghe VR, Corrie JE.
Journal: Biochemistry (2004): 14463
Fluorescent coumarin-labeled nucleotides to measure ADP release from actomyosin
Authors: Webb MR, Corrie JE.
Journal: Biophys J (2001): 1562
Glutamate 779, an intramembrane carboxyl, is essential for monovalent cation binding by the Na,K-ATPase
Authors: Arguello JM, Kaplan JH.
Journal: J Biol Chem (1994): 6892