Name: DEAC,SE [7-Diethylaminocoumarin-3-carboxylic acid, succinimidyl ester] *CAS 139346-57-9*
Brand: AAT Bioquest
SKU: 506
Price: 115 USD
Availability: InStock

DEAC,SE

7-Diethylaminocoumarin-3-carboxylic acid, succinimidyl ester; CAS 139346-57-9

The amine-reactive 7-diethylaminocoumarin-3-carboxylic acid, succinimidyl ester can be used to create blue-fluorescent bioconjugates. It has quite strong blue fluorescence. When compared with AMCA conjugates, conjugates of the UV-light-excitable 7-dialkylaminocoumarin fluorophore have much longer-wavelength excitation and slightly longer-wavelength emission spectra (~470 nm). This fluorescent dye is quite hydrophobic, and might be used for labeling live cells.

Protocol

SDS

Catalog	Size	Price	Quantity
506	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DEAC,SE [7-Diethylaminocoumarin-3-carboxylic acid, succinimidyl ester] *CAS 139346-57-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	279.057 µL	1.395 mL	2.791 mL	13.953 mL	27.906 mL
5 mM	55.811 µL	279.057 µL	558.114 µL	2.791 mL	5.581 mL
10 mM	27.906 µL	139.528 µL	279.057 µL	1.395 mL	2.791 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	358.35 g/mol	=	mL	x	mM	=	mmol

References

View all 4 references: Citation Explorer

Fluorescent agonists for the Torpedo nicotinic acetylcholine receptor

Authors:

Krieger F, Mourot A, Araoz R, Kotzyba-Hibert F, Molgo J, Bamberg E, Goeldner M.

Journal:

Chembiochem (2008): 1146

A series of related nucleotide analogues that aids optimization of fluorescence signals in probing the mechanism of P-loop ATPases, such as actomyosin

Authors:

Webb MR, Reid GP, Munasinghe VR, Corrie JE.

Journal:

Biochemistry (2004): 14463

Fluorescent coumarin-labeled nucleotides to measure ADP release from actomyosin

Authors:

Webb MR, Corrie JE.

Journal:

Biophys J (2001): 1562

Glutamate 779, an intramembrane carboxyl, is essential for monovalent cation binding by the Na,K-ATPase

Authors:

Arguello JM, Kaplan JH.

Journal:

J Biol Chem (1994): 6892

Physical properties

Molecular weight	358.35
Solvent	DMSO

Spectral properties

Correction factor (260 nm)	0.14
Correction factor (280 nm)	0.12
Excitation (nm)	411
Emission (nm)	472

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501
CAS	139346-57-9

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Oligonucleotide Labeling Reagents
Brochure: Peptide Labeling Reagents

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