Di-2-ANEPEQ [JPW 1114]

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Physical properties

Molecular weight	549.38
Solvent	Water

Spectral properties

Excitation (nm)	488
Emission (nm)	701

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

Molecular weight

549.38

Excitation (nm)

488

Emission (nm)

701

Di-2-ANEPEQ is used for monitoring fast membrane potential changes. ANEP dyes belong to the class of the fast-response membrane potential dyes. Their optical response is fast enough to detect transient membrane potential changes in excitable cells where they demonstrate a membrane potential-dependent shift in excitation spectra. This feature allows the measurement of membrane potential changes by excitation ratio. These dyes are weakly fluorescent in aqueous media, and become strongly fluorescent upon binding to lipophilic environments (such as membranes). In general, fast-response probes operate by means of a change in their electronic structure, and consequently their fluorescence properties, in response to a change in the surrounding electric field. Their optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells and intact brains. However, the magnitude of their potential-dependent fluorescence change is often small; fast-response probes typically show a 2-10% fluorescence change per 100 mV.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Di-2-ANEPEQ [JPW 1114] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	182.023 µL	910.117 µL	1.82 mL	9.101 mL	18.202 mL
5 mM	36.405 µL	182.023 µL	364.047 µL	1.82 mL	3.64 mL
10 mM	18.202 µL	91.012 µL	182.023 µL	910.117 µL	1.82 mL

Molarity calculator

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Spectrum

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Spectral properties

Excitation (nm)	488
Emission (nm)	701

Images

Figure 1. Chemical structure for Di-2-ANEPEQ [JPW 1114]

Citations

View all 1 citations: Citation Explorer

Cholesterol is required for maintaining T-tubule integrity and intercellular connections at intercalated discs in cardiomyocytes
Authors: Zhu, Yanqi and Zhang, Caimei and Chen, Biyi and Chen, Rong and Guo, Ang and Hong, Jiang and Song, Long-Sheng
Journal: Journal of molecular and cellular cardiology (2016): 204--212

References

View all 109 references: Citation Explorer

Optical imaging of medullary ventral respiratory network during eupnea and gasping in situ
Authors: Potts JT, Paton JF.
Journal: Eur J Neurosci (2006): 3025

Membrane dipole potential as measured by ratiometric 3-hydroxyflavone fluorescence probes: accounting for hydration effects
Authors: M'Baye G, Shynkar VV, Klymchenko AS, Mely Y, Duportail G.
Journal: J Fluoresc (2006): 35

Cholesterol effect on the dipole potential of lipid membranes
Authors: Starke-Peterkovic T, Turner N, Vitha MF, Waller MP, Hibbs DE, Clarke RJ.
Journal: Biophys J (2006): 4060

Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells
Authors: Baker BJ, Lee H, Pieribone VA, Cohen LB, Isacoff EY, Knopfel T, Kosmidis EK.
Journal: J Neurosci Methods. (2006)

Imaging of cardiac movement using ratiometric and nonratiometric optical mapping: effects of ischemia and 2, 3-butaneodione monoxime
Authors: Himel HDt, Knisley SB.
Journal: IEEE Trans Med Imaging (2006): 122

Effect of Cholesterol on the Interaction of the HIV GP41 Fusion Peptide with Model Membranes. Importance of the Membrane Dipole Potential
Authors: Buzon V, Cladera J.
Journal: Biochemistry (2006): 15768

Membrane potential of rat ventricular myocytes responds to axial stretch in phase, amplitude and speed-dependent manners
Authors: Nishimura S, Kawai Y, Nakajima T, Hosoya Y, Fujita H, Katoh M, Yamashita H, Nagai R, Sugiura S.
Journal: Cardiovasc Res (2006): 403

The Gurvich waveform has lower defibrillation threshold than the rectilinear waveform and the truncated exponential waveform in the rabbit heart
Authors: Qu F, Zarubin F, Wollenzier B, Nikolski VP, Efimov IR.
Journal: Can J Physiol Pharmacol (2005): 152

Near infrared two-photon excitation cross-sections of voltage-sensitive dyes
Authors: Fisher JA, Salzberg BM, Yodh AG.
Journal: J Neurosci Methods (2005): 94

Characteristics of a charged-coupled-device-based optical mapping system for the study of cardiac arrhythmias
Authors: Tang D, Li Y, Wong J, Po S, Patterson E, Chen WR, Jackman W, Liu H.
Journal: J Biomed Opt (2005): 24009