Name: Di-2-ANEPEQ [JPW 1114]
Brand: AAT Bioquest
SKU: 21496
Price: 232 USD
Availability: InStock

Di-2-ANEPEQ

JPW 1114

Di-2-ANEPEQ is used for monitoring fast membrane potential changes. ANEP dyes belong to the class of the fast-response membrane potential dyes. Their optical response is fast enough to detect transient membrane potential changes in excitable cells where they demonstrate a membrane potential-dependent shift in excitation spectra. This feature allows the measurement of membrane potential changes by excitation ratio. These dyes are weakly fluorescent in aqueous media, and become strongly fluorescent upon binding to lipophilic environments (such as membranes). In general, fast-response probes operate by means of a change in their electronic structure, and consequently their fluorescence properties, in response to a change in the surrounding electric field. Their optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells and intact brains. However, the magnitude of their potential-dependent fluorescence change is often small; fast-response probes typically show a 2-10% fluorescence change per 100 mV.

Catalog	Size	Price	Quantity
21496	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of Di-2-ANEPEQ [JPW 1114] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	182.023 µL	910.117 µL	1.82 mL	9.101 mL	18.202 mL
5 mM	36.405 µL	182.023 µL	364.047 µL	1.82 mL	3.64 mL
10 mM	18.202 µL	91.012 µL	182.023 µL	910.117 µL	1.82 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	549.38 g/mol	=	mL	x	mM	=	mmol

References

View all 109 references: Citation Explorer

Optical imaging of medullary ventral respiratory network during eupnea and gasping in situ

Authors:

Potts JT, Paton JF.

Journal:

Eur J Neurosci (2006): 3025

Membrane dipole potential as measured by ratiometric 3-hydroxyflavone fluorescence probes: accounting for hydration effects

Authors:

M'Baye G, Shynkar VV, Klymchenko AS, Mely Y, Duportail G.

Journal:

J Fluoresc (2006): 35

Cholesterol effect on the dipole potential of lipid membranes

Authors:

Starke-Peterkovic T, Turner N, Vitha MF, Waller MP, Hibbs DE, Clarke RJ.

Journal:

Biophys J (2006): 4060

Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells

Authors:

Baker BJ, Lee H, Pieribone VA, Cohen LB, Isacoff EY, Knopfel T, Kosmidis EK.

Journal:

J Neurosci Methods. (2006)

Imaging of cardiac movement using ratiometric and nonratiometric optical mapping: effects of ischemia and 2, 3-butaneodione monoxime

Authors:

Himel HDt, Knisley SB.

Journal:

IEEE Trans Med Imaging (2006): 122

Physical properties

Molecular weight	549.38
Solvent	Water

Spectral properties

Excitation (nm)	488
Emission (nm)	701

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

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Page updated on October 13, 2025