Di-4-ANBDQBS [JPW6033] | AAT Bioquest

Di-4-ANBDQBS

JPW6033

Di-4-ANEPPS is considered a "gold standard" of optical mapping membrane potentials. However, its short wavelength, higher internalization rate and small fluorescence change limit its use in some biological samples. Di-4-ANBDQPQ and Di-4-ANBDQBS demonstrated 50-100% deltaF/F signal enhancement than di-4-ANEPPS in Tyrode's perfused ventricular wall preparations. The longer excitation wavelength of Di-4-ANBDQPQ and Di-4-ANBDQBS is well separated from the absorption maximum of blood (~580 nm), conveying a major advantage in blood-perfused preparations. Di-4-ANBDQPQ and Di-4ANBDQBS increase the depth of imaging of electrical activity in tissues, and facilitate imaging during blood perfusion. These two dyes are also useful in conventional epicardial mapping applications. In summary, Di-4-ANBDQPQ and Di-4ANBDQBS have greater ?F/F, slower internalization rate and easy cell loading. Moreover, the red shift of excitation and emission is likely to be another useful feature. These dyes may as well be useful for optical mapping of monolayers and individual cardiac myocytes, given the ability of these dyes to respond to low levels of illumination (1 mW/mm2) and high deltaF/F. Monolayers and individual cardiac myocytes usually require high intensities of illumination, which often results in photobleaching.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21500	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Di-4-ANBDQBS [JPW6033] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	175.199 µL	875.994 µL	1.752 mL	8.76 mL	17.52 mL
5 mM	35.04 µL	175.199 µL	350.398 µL	1.752 mL	3.504 mL
10 mM	17.52 µL	87.599 µL	175.199 µL	875.994 µL	1.752 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	570.78 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

Light transmittance in human atrial tissue and transthoracic illumination in rats support translatability of optogenetic cardioversion of atrial fibrillation

Authors:

Nyns, Emile CA and Portero, Vincent and Deng, Shanliang and Jin, Tianyi and Harlaar, Niels and Bart, Cindy I and van Brakel, Thomas J and Palmen, Meindert and Hjortnaes, Jesper and Ramkisoensing, Arti A and others,

Journal:

Journal of Internal Medicine (2023)

Antiarrhythmic Properties of Elsholtzia ciliata Essential Oil on Electrical Activity of the Isolated Rabbit Heart and Preferential Inhibition of Sodium Conductance

Authors:

Ma{\v{c}}ianskien{\.e}, Regina and Pud{\v{z}}iuvelyt{\.e}, Lauryna and Bernatonien{\.e}, Jurga and Almanaityt{\.e}, Mant{\.e} and Navalinskas, Antanas and Treinys, Rimantas and Andriul{\.e}, Inga and Jurevi{\v{c}}ius, Jonas

Journal:

Biomolecules (2020): 948

Preliminary findings on ultrasound modulation of the electromechanical function of human stem-cell-derived cardiomyocytes

Authors:

Chen, Andrew William and Klimas, Aleksandra and Zderic, Vesna and Castellanos, Ivan Suares and Entcheva, Emilia

Preliminary findings on ultrasound modulation of the electromechanical function of human stem-cell-derived cardiomyocytes

Authors:

Chen, Andrew William and Klimas, Aleks and ra , undefined and Zderic, Vesna and Castellanos, Ivan Suares and Entcheva, Emilia

References

View all 3 references: Citation Explorer

Simultaneous measurement and modulation of multiple physiological parameters in the isolated heart using optical techniques

Authors:

Lee P, Yan P, Ewart P, Kohl P, Loew LM, Bollensdorff C.

Journal:

Pflugers Arch (2012): 403

Single-sensor system for spatially resolved, continuous, and multiparametric optical mapping of cardiac tissue

Authors:

Lee P, Bollensdorff C, Quinn TA, Wuskell JP, Loew LM, Kohl P.

Journal:

Heart Rhythm (2011): 1482

Near-infrared voltage-sensitive fluorescent dyes optimized for optical mapping in blood-perfused myocardium

Authors:

Matiukas A, Mitrea BG, Qin M, Pertsov AM, Shvedko AG, Warren MD, Zaitsev AV, Wuskell JP, Wei MD, Watras J, Loew LM.

Journal:

Heart Rhythm (2007): 1441

Physical properties

Molecular weight	570.78
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on July 26, 2023