Name: Di-4-ANEPPS *CAS#: 90134-00-2*
Brand: AAT Bioquest
SKU: 21498
Price: 232 USD
Availability: InStock

Di-4-ANEPPS

CAS#: 90134-00-2

Potential-sensitive ANEP dyes are zwitterionic molecule, exhibiting the most consistent potentiometric response in different cell and tissue types. They can be introduced into cells by direct addition of stock solution to cell culture medium, by using Pluronic® F-127, or by retrograde labeling. ANEP dyes are non-fluorescent until bound to membranes, and their fluoresce responses to electrical potential changes in their environment. ANEP dyes are fast-response probes that operate by means of a change in their electronic structure, and consequently their fluorescence properties, in response to a change in the surrounding electric field. Their optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells, and intact brains. However, the magnitude of their potential-dependent fluorescence change is often small; fast-response probes typically show a 2-10% fluorescence change per 100 mV. Furthermore, these dyes display a potential-dependent shift in their excitation spectra, thus permitting the quantitation of membrane potential using excitation ratio measurements.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
21498	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Di-4-ANEPPS *CAS#: 90134-00-2* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	208.047 µL	1.04 mL	2.08 mL	10.402 mL	20.805 mL
5 mM	41.609 µL	208.047 µL	416.095 µL	2.08 mL	4.161 mL
10 mM	20.805 µL	104.024 µL	208.047 µL	1.04 mL	2.08 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	480.66 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

SNAP25-dependent membrane trafficking of the Kv1. 5 channel regulates the onset of atrial fibrillation

Authors:

Su, Xuling and Shao, Beihua and Chen, Zhiwen and Gu, Hongcheng and Xiong, Ke and Wang, Guanghua and Zou, Qicheng and Cao, Yuting and Zhang, Caihong and Xu, Hongtao and others,

Journal:

Nature Communications (2025): 3730

High-speed optical mapping of heart and brain voltage activities in zebrafish larvae exposed to environmental contaminants

Authors:

Micou, Solene and Forner-Piquer, Isabel and Cresto, Noemie and Zassot, Tess and Drouard, Aur{\'e}lien and Larbi, Marianne and Mangoni, Matteo E and Audinat, Etienne and Jopling, Chris and Faucherre, Ad{\`e}le and others,

Journal:

Environmental Technology \& Innovation (2023): 103196

References

View all 11 references: Citation Explorer

The voltage-sensitive dye di-4-ANEPPS slows conduction velocity in isolated guinea pig hearts

Authors:

Larsen AP, Sciuto KJ, Moreno AP, Poelzing S.

Journal:

Heart Rhythm (2012): 1493

A novel approach to dual excitation ratiometric optical mapping of cardiac action potentials with di-4-ANEPPS using pulsed LED excitation

Authors:

Bachtel AD, Gray RA, Stohlman JM, Bourgeois EB, Pollard AE, Rogers JM.

Journal:

IEEE Trans Biomed Eng (2011): 2120

Potential-modulated fluorescence spectroscopy of the membrane potential-sensitive dye di-4-ANEPPS at the 1,2-dichloroethane/water interface

Authors:

Osakai T, Sawada J, Nagatani H.

Journal:

Anal Bioanal Chem (2009): 1055

Validation of a voltage-sensitive dye (di-4-ANEPPS)-based method for assessing drug-induced delayed repolarisation in beagle dog left ventricular midmyocardial myocytes

Authors:

Hardy ME, Pollard CE, Small BG, Bridgl and -Taylor M, Woods AJ, Valentin JP, Abi-Gerges N.

Journal:

J Pharmacol Toxicol Methods (2009): 94

Effects of voltage sensitive dye di-4-ANEPPS on guinea pig and rabbit myocardium

Authors:

Novakova M, Bardonova J, Provaznik I, Taborska E, Bochorakova H, Paulova H, Horky D.

Journal:

Gen Physiol Biophys (2008): 45

Physical properties

Molecular weight	480.66
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	31622
Excitation (nm)	482
Emission (nm)	686

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	90134-00-2

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Page updated on October 13, 2025