Di-8-ANEPPS *CAS#: 157134-53-7*

Di-8-ANEPPS

CAS#: 157134-53-7

Di-8-ANEPPS is widely used for monitoring fast membrane potential changes. ANEP dyes belong to the class of the fast-response membrane potential dyes. Their optical response is fast enough to detect transient membrane potential changes in excitable cells where they demonstrate a membrane potential-dependent shift in excitation spectra. This feature allows the measurement of membrane potential changes by excitation ratio. These dyes are weakly fluorescent in aqueous media, and become strongly fluorescent upon binding to lipophilic environments (such as membranes). Di-8-ANEPPS is less susceptible for cellular internalization than other ANEP dyes probably due to its sulfonate group. In general, fast-response probes operate by means of a change in their electronic structure, and consequently their fluorescence properties, in response to a change in the surrounding electric field. Their optical response is sufficiently fast to detect transient (millisecond) potential changes in excitable cells, including single neurons, cardiac cells and intact brains. However, the magnitude of their potential-dependent fluorescence change is often small; fast-response probes typically show a 2-10% fluorescence change per 100 mV.

Catalog	Size	Price	Quantity
21497	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Di-8-ANEPPS *CAS#: 157134-53-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	168.668 µL	843.341 µL	1.687 mL	8.433 mL	16.867 mL
5 mM	33.734 µL	168.668 µL	337.336 µL	1.687 mL	3.373 mL
10 mM	16.867 µL	84.334 µL	168.668 µL	843.341 µL	1.687 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	592.88 g/mol	=	mL	x	mM	=	mmol

Citations

View all 4 citations: Citation Explorer

Destructive role of TMAO in T-tubule and excitation-contraction coupling in the adult cardiomyocytes

Authors:

Jin, Bu and Ji, Fangfang and Zuo, Anjun and Liu, Huiting and Qi, Lin and He, Yun and Wang, Qingyao and Zhao, Peng

Journal:

International Heart Journal (2020): 355--363

Cholesterol is required for maintaining T-tubule integrity and intercellular connections at intercalated discs in cardiomyocytes

Authors:

Zhu, Yanqi and Zhang, Caimei and Chen, Biyi and Chen, Rong and Guo, Ang and Hong, Jiang and Song, Long-Sheng

Journal:

Journal of molecular and cellular cardiology (2016): 204--212

Preserving transverse tubule function in adult cardiac myocytes in short-term culture.

Authors:

Young, Kester EJ and Young, Kester EJ

Journal:

Cardiol : 804--812

Multiple Functions for Targeted Self-delivery and Cancer Treatments

Authors:

Luo, Shi-Zhong

References

View all 109 references: Citation Explorer

Optical imaging of medullary ventral respiratory network during eupnea and gasping in situ

Authors:

Potts JT, Paton JF.

Journal:

Eur J Neurosci (2006): 3025

Membrane dipole potential as measured by ratiometric 3-hydroxyflavone fluorescence probes: accounting for hydration effects

Authors:

M'Baye G, Shynkar VV, Klymchenko AS, Mely Y, Duportail G.

Journal:

J Fluoresc (2006): 35

Cholesterol effect on the dipole potential of lipid membranes

Authors:

Starke-Peterkovic T, Turner N, Vitha MF, Waller MP, Hibbs DE, Clarke RJ.

Journal:

Biophys J (2006): 4060

Three fluorescent protein voltage sensors exhibit low plasma membrane expression in mammalian cells

Authors:

Baker BJ, Lee H, Pieribone VA, Cohen LB, Isacoff EY, Knopfel T, Kosmidis EK.

Journal:

J Neurosci Methods. (2006)

Imaging of cardiac movement using ratiometric and nonratiometric optical mapping: effects of ischemia and 2, 3-butaneodione monoxime

Authors:

Himel HDt, Knisley SB.

Journal:

IEEE Trans Med Imaging (2006): 122

Physical properties

Molecular weight	592.88
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	31622
Excitation (nm)	467
Emission (nm)	631

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	157134-53-7

Documents

Protocol
Safety Data Sheet
Certificate of Origin
Certificate of Analysis

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Page updated on October 24, 2025