Name: Diazepam Biotin Conjugate
Brand: AAT Bioquest
SKU: 50664
Price: 989 USD
Availability: InStock

Diazepam Biotin Conjugate

Diazepam biotin conjugate is a biotinylated benzodiazepine derivative that combines GABAA receptor binding with high-affinity streptavidin interaction, enabling affinity purification and detection of benzodiazepine-binding proteins.

GABAA Receptor Binding Preserved: Maintains the pharmacophore of diazepam for accurate target-specific studies and functional assays
Streptavidin-Based Detection: Enables sensitive receptor-ligand interaction assays including pull-downs, ELISAs, and biosensor platforms
High-Throughput Compatible: Adaptable to automated screening workflows for drug discovery and GABAergic signaling studies
Dual-Functionality Advantage: Combines benzodiazepine activity with biotin affinity for superior versatility over standard diazepam probes

The chemical structure of Diazepam Biotin Conjugate.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
50664	1 mg	Price

Overview

Diazepam Biotin Conjugate is a structurally defined, biotinylated analog of diazepam engineered for affinity-based applications in neuropharmacological and biochemical research. The molecule features a stable covalent linkage between diazepam and biotin via a suitable spacer, designed to preserve the pharmacologically active benzodiazepine core while enabling high-affinity interaction with streptavidin, avidin, or neutravidin matrices. This dual functionality allows for the selective capture, enrichment, or immobilization of GABAA receptor subunits or diazepam-binding proteins in complex biological samples. The conjugate is particularly useful in receptor-ligand binding assays, affinity purification, and target deconvolution workflows, where it facilitates sensitive and specific interrogation of GABAergic signaling pathways. Its compatibility with biotin-binding platforms also supports its use in biosensor development and high-throughput screening for drug discovery.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Diazepam Biotin Conjugate to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	100 mL	500 mL	1 L	5 L	10 L
5 mM	20 mL	100 mL	200 mL	1 L	2 L
10 mM	10 mL	50 mL	100 mL	500 mL	1 L

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1 g/mol	=	mL	x	mM	=	mmol

References

View all 8 references: Citation Explorer

Acyl-CoA binding protein for the experimental treatment of anorexia.

Authors:

Chen, Hui and Moriceau, Stéphanie and Joseph, Adrien and Mailliet, Francois and Li, Sijing and Tolle, Virginie and Duriez, Philibert and Dardennes, Roland and Durand, Sylvère and Carbonnier, Vincent and Stoll, Gautier and Sauvat, Allan and Lachkar, Sylvie and Aprahamian, Fanny and Alves Costa Silva, Carolina and Pan, Hui and Montégut, Léa and Anagnostopoulos, Gerasimos and Lambertucci, Flavia and Motiño, Omar and Nogueira-Recalde, Uxía and Bourgin, Mélanie and Mao, Misha and Pan, Yuhong and Cerone, Alexandra and Boedec, Erwan and Gouveia, Zelia L and Marmorino, Federica and Cremolini, Chiara and Derosa, Lisa and Zitvogel, Laurence and Kepp, Oliver and López-Otín, Carlos and Maiuri, Maria Chiara and Perez, Franck and Gorwood, Philip and Ramoz, Nicolas and Oury, Franck and Martins, Isabelle and Kroemer, Guido

Journal:

Science translational medicine (2024): eadl0715

Regulation of anxiety-like behaviors by S-palmitoylation and S-nitrosylation in basolateral amygdala.

Authors:

Shen, Zu-Cheng and Liu, Jian-Min and Zheng, Jie-Yan and Li, Meng-Die and Tian, Dan and Pan, Yue and Tao, Wu-Cheng and Gao, Shuang-Qi and Xia, Zhi-Xuan

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie (2023): 115859

Gephyrin Palmitoylation in Basolateral Amygdala Mediates the Anxiolytic Action of Benzodiazepine.

Authors:

Shen, Zu-Cheng and Wu, Peng-Fei and Wang, Fang and Xia, Zhi-Xuan and Deng, Qiao and Nie, Tai-Lei and Zhang, Shao-Qi and Zheng, Hui-Ling and Liu, Wen-Hui and Lu, Jia-Jing and Gao, Shuang-Qi and Yao, Xia-Ping and Long, Li-Hong and Hu, Zhuang-Li and Chen, Jian-Guo

Journal:

Biological psychiatry (2019): 202-213

Excitatory tonus is required for the survival of granule cell precursors during postnatal development within the cerebellum.

Authors:

Kanungo, A K and Liadis, N and Robertson, J and Woo, M and Henderson, J T

Journal:

Neuroscience (2009): 1364-77

Spatio-temporal profile of DNA fragmentation and its relationship to patterns of epileptiform activity following focally evoked limbic seizures.

Authors:

Henshall, D C and Sinclair, J and Simon, R P

Journal:

Brain research (2000): 290-302

Physical properties

Molecular weight	1 mg
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on March 4, 2026