Name: Diazepam NHS ester
Brand: AAT Bioquest
SKU: 50660
Price: 989 USD
Availability: InStock

Diazepam NHS ester

Diazepam NHS ester is an amine-reactive benzodiazepine derivative that forms stable amide bonds with primary amines under mild conditions, facilitating conjugation to proteins while preserving GABAA receptor binding activity.

Amine-Reactive NHS ester: Enables efficient and selective conjugation to primary amines, forming stable amide bonds under mild aqueous conditions
Biologically Active Diazepam Core: Preserves the structural and pharmacological properties of diazepam, including affinity for GABAA receptors
Neuropharmacological Applications: Supports the development of molecular probes, affinity ligands, and receptor-binding assays
Versatile Bioconjugation Platform: Compatible with protein labeling, immunoassay development, and targeted drug delivery systems

The chemical structure of Diazepam NHS ester.

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
50660	1 mg	Price

Overview

Diazepam NHS ester is a chemically activated derivative of diazepam incorporating an N-hydroxysuccinimide (NHS) ester functional group for targeted bioconjugation. The NHS ester reacts selectively with primary amines under physiological to slightly basic conditions (pH 7.0–8.5), enabling the formation of stable amide bonds with proteins, peptides, and other amine-containing biomolecules. The diazepam scaffold remains intact, preserving its affinity for GABA_A receptors and related pharmacological targets. This reagent is well-suited for the development of diazepam-functionalized affinity ligands, targeted delivery systems, and molecular probes for receptor-binding assays and imaging applications. Its compatibility with aqueous systems and high coupling efficiency make Diazepam NHS ester a useful tool in neuropharmacological research, investigating benzodiazepine mechanisms, receptor-ligand interaction studies, and the development of functionalized delivery platforms or diagnostic probes.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Diazepam NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	207.499 µL	1.037 mL	2.075 mL	10.375 mL	20.75 mL
5 mM	41.5 µL	207.499 µL	414.998 µL	2.075 mL	4.15 mL
10 mM	20.75 µL	103.75 µL	207.499 µL	1.037 mL	2.075 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	481.93 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Multiplex immunochromatographic assay based on triple-color aggregation-induced emission fluorescent microspheres with good biocompatibility for the simultaneous detection of veterinary drugs in aquatic products.

Authors:

Su, Liu and Lai, Xiaocui and Zhang, Ganggang and Liu, Cong and He, Weihua and Zhang, Gan and Lai, Weihua and Deng, Shengliang

Journal:

Analytica chimica acta (2025): 343700

The open field assay is influenced by room temperature and by drugs that affect core body temperature.

Authors:

Jimenez, Jessica A and McCoy, Eric S and Lee, David F and Zylka, Mark J

Journal:

F1000Research (2023): 234

Novel 2-substituted-5-(4-chloro-2-phenoxy)phenyl-1,3,4-oxadiazole derivatives, ligands of GABAA/benzodiazepine receptor complex: Design, synthesis, radioligand binding assay, and pharmacological evaluation.

Authors:

Rezaee, Elham and Ahmadi, Fatemeh and Shabaninia, Mahsa and Khoramjouy, Mona and Azizi Farsani, Zahra and Shahhosseini, Soraya and Tabatabai, Sayyed Abbas and Faizi, Mehrdad

Journal:

EXCLI journal (2023): 250-262

A high-throughput bioanalytical assay to support pharmacokinetic interaction study of oxycodone and diazepam in Sprague Dawley rats.

Authors:

Pilli, Nageswara R and Narayanasamy, Suresh and Xu, Lin and Chockalingam, Ashok and Shea, Katherine I and Stewart, Sharron and Rouse, Rodney and Patel, Vikram and Matta, Murali K

Journal:

RSC advances (2020): 886-896

Synthesis and radioligand-binding assay of 2,5-disubstituted thiadiazoles and evaluation of their anticonvulsant activities.

Authors:

Toolabi, Mahsa and Khoramjouy, Mona and Aghcheli, Ayoub and Ayati, Adileh and Moghimi, Setareh and Firoozpour, Loghman and Shahhosseini, Soraya and Shojaei, Rouhallah and Asadipour, Ali and Divsalar, Kouros and Faizi, Mehrdad and Foroumadi, Alireza

Journal:

Archiv der Pharmazie (2020): e2000066

Physical properties

Molecular weight	481.93
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on May 9, 2026