Diazepam NHS ester

• Amine-reactive NHS ester – Enables efficient & selective conjugation to primary amines, forming stable amide bonds under mild aqueous conditions.
• Biologically active diazepam core – Preserves the structural and pharmacological properties of diazepam, including affinity for GABA_A receptors.
• Ideal for neuropharmacological applications – Supports the development of molecular probes, affinity ligands, & receptor-binding assays.

Diazepam NHS ester is a chemically activated derivative of diazepam incorporating an N-hydroxysuccinimide (NHS) ester functional group for targeted bioconjugation. The NHS ester reacts selectively with primary amines under physiological to slightly basic conditions (pH 7.0–8.5), enabling the formation of stable amide bonds with proteins, peptides, and other amine-containing biomolecules. The diazepam scaffold remains intact, preserving its affinity for GABA_A receptors and related pharmacological targets. This reagent is well-suited for the development of diazepam-functionalized affinity ligands, targeted delivery systems, and molecular probes for receptor-binding assays and imaging applications. Its compatibility with aqueous systems and high coupling efficiency make Diazepam NHS ester a useful tool in neuropharmacological research, investigating benzodiazepine mechanisms, receptor-ligand interaction studies, and the development of functionalized delivery platforms or diagnostic probes.