DiD Dye | AAT Bioquest

DiD labeling solution

1,1-Dioctadecyl-3,3,3,3-tetramethylindodicarbocyanine; 5 mM DMSO solution

DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
22033	10 mL	Price

Citations

View all 62 citations: Citation Explorer

Observation of antitumor mechanism of GE11-modified paclitaxel and curcumin liposomes based on cellular morphology changes

Authors:

Tang, Hailing and Li, Lijuan and Wang, Baoshan and Guangxi Scientific Research Center of Traditional Chinese Medicine,

Journal:

AAPS Open (2024): 1

An oncolytic virus--T cell chimera for cancer immunotherapy

Authors:

Chen, Yuxuan and Chen, Xiaohong and Bao, Weier and Liu, Gang and Wei, Wei and Ping, Yuan

Journal:

Nature Biotechnology (2024): 1--12

Hyperglycemia-induced endothelial exosomes trigger trophoblast dysregulation and abnormal placentation through PUM2-mediated repression of SOX2

Authors:

Zhu, Aibing and Qi, Suwan and Li, Wenjuan and Chen, Dashu and Zheng, Xiaomin and Xu, Jianjuan and Feng, Yaling

Journal:

Human \& Experimental Toxicology (2023): 09603271221149656

Enhanced osteoarthritis therapy by nanoengineered mesenchymal stem cells using biomimetic CuS nanoparticles loaded with plasmid DNA encoding TGF-$\beta$1

Authors:

Cai, Yu and Wu, Cuixi and Ou, Qianhua and Zeng, Muhui and Xue, Song and Chen, Jieli and Lu, Yao and Ding, Changhai

Journal:

Bioactive materials (2023): 444--457

Ibuprofen in Therapeutic Concentrations Affects the Secretion of Human Bone Marrow Mesenchymal Stromal Cells, but Not Their Proliferative and Migratory Capacity

Authors:

Kulesza, Agnieszka and Zielniok, Katarzyna and Hawryluk, Jakub and Paczek, Leszek and Burdzinska, Anna

Journal:

Biomolecules (2022): 287

References

View all 92 references: Citation Explorer

In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope

Authors:

Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.

Journal:

Invest Ophthalmol Vis Sci (2006): 2943

Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials

Authors:

Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.

Journal:

J Biomed Mater Res B Appl Biomater. (2006)

Functional neuroanatomy of the rhinophore of Aplysia punctata

Authors:

Wertz A, Rossler W, Obermayer M, Bickmeyer U.

Journal:

Front Zool (2006): 6

Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius

Authors:

Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.

Journal:

Hypertension (2006): 482

Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing

Authors:

Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.

Journal:

J Biomed Opt (2006): 50507

Physical properties

Molecular weight	959.91
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	260000
Excitation (nm)	646
Emission (nm)	663

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	127274-91-3

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Page updated on August 13, 2024