Name: Digoxigenin-11-dUTP *1 mM solution in TE Buffer*
Brand: AAT Bioquest
SKU: 17012
Price: 174 USD
Availability: InStock

Digoxigenin-11-dUTP

1 mM solution in TE Buffer

Digoxigenin-11-dUTP can be enzymatically incorporated into DNA/cDNA as substitute for its natural counterpart dTTP. The resulting digoxigenin-labeled DNA/cDNA probes are subsequently detected using digoxigenin-antibodies conjugated with horseradish peroxidase (HRP), alkaline phosphatase (AP) or a fluorescent dye. The 11-atom linker is attached to the C5 position of uridine to minimize the interference of DIG on the substrate incorporation by PCR polymerase. Typically1:2 Digoxigenin-11-dUTP/dTTP ratio for PCR and Nick Translation.

Catalog	Size	Price	Quantity
17012	25 nmoles	Price

Citations

View all 2 citations: Citation Explorer

LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis

Authors:

Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua

Journal:

Biochemical and Biophysical Research Communications (2021): 95--102

ATF4-mediated transcriptional regulation protects against $\beta$-cell loss during endoplasmic reticulum stress in a mouse model

Authors:

Kitakaze, Keisuke and Oyadomari, Miho and Zhang, Jun and Hamada, Yoshimasa and Takenouchi, Yasuhiro and Tsuboi, Kazuhito and Inagaki, Mai and Tachikawa, Masanori and Fujitani, Yoshio and Okamoto, Yasuo and others,

Journal:

Molecular Metabolism (2021): 101338

References

View all 9 references: Citation Explorer

A visual DNA chip for simultaneous detection of hepatitis B virus, hepatitis C virus and human immunodeficiency virus type-1

Authors:

Wen JK, Zhang XE, Cheng Z, Liu H, Zhou YF, Zhang ZP, Yang JH, Deng JY.

Journal:

Biosens Bioelectron (2004): 685

Fluorescent DNA hybridization probe preparation using amine modification and reactive dye coupling

Authors:

Cox WG, Singer VL.

Journal:

Biotechniques (2004): 114

Aminomodified nucleobases: functionalized nucleoside triphosphates applicable for SELEX

Authors:

Schoetzau T, Langner J, Moyroud E, Roehl I, Vonhoff S, Klussmann S.

Journal:

Bioconjug Chem (2003): 919

Topology of yeast RNA polymerase II subunits in transcription elongation complexes studied by photoaffinity cross-linking

Authors:

Wooddell CI, Burgess RR.

Journal:

Biochemistry (2000): 13405

Simple method for preparation of fluor/hapten-labeled dUTP

Authors:

Nimmakayalu M, Henegariu O, Ward DC, Bray-Ward P.

Journal:

Biotechniques (2000): 518

Physical properties

Molecular weight	1132.87
Solvent	Water

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	129030-36-0

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