Digoxigenin alkyne

Digoxigenin (DIG) is a hapten, a small molecule with high antigenicity, which is used in many biological detections similarly to other popular haptens such as 2,4-Dinitrophenol (DNP) and biotin. Typically, digoxigenin is conjugated to a macrobiomolecule (e.g., proteins, nucleic acids, etc) to be detected. Anti-digoxigenin antibodies are labeled with fluorescent dyes or enzymes, directly or secondarily, for visualization and detection. Digoxigenin alkyne readily reacts with azido group under mild conditions, attaching the digoxigenin moiety to azido-containing molecules. Digoxigenin-labled biomolecules are used for biological detections in combination with anti-digoxigenin antibodies of high affinity and specificity.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
3505	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Digoxigenin alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	205.922 µL	1.03 mL	2.059 mL	10.296 mL	20.592 mL
5 mM	41.184 µL	205.922 µL	411.845 µL	2.059 mL	4.118 mL
10 mM	20.592 µL	102.961 µL	205.922 µL	1.03 mL	2.059 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	485.62 g/mol	=	mL	x	mM	=	mmol

Citations

View all 21 citations: Citation Explorer

LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis

Authors:

Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua

Journal:

Biochemical and Biophysical Research Communications (2021): 95--102

A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin

Authors:

Ruppert, C., Phogat, N., Laufer, S., Kohl, M., Deigner, H. P.

Journal:

Mikrochim Acta (2019): 119

Application of Antibody-Powered Triplex-DNA Nanomachine to Electrochemiluminescence Biosensor for the Detection of Anti-Digoxigenin with Improved Sensitivity Versus Cycling Strand Displacement Reaction

Authors:

Yang, S. S., Jiang, M. H., Chai, Y. Q., Yuan, R., Zhuo, Y.

Journal:

ACS Appl Mater Interfaces (2018): 38648-38655

Synthesis of C3-Neoglycosides of digoxigenin and their anticancer activities

Authors:

Li, X. S., Ren, Y. C., Bao, Y. Z., Liu, J., Zhang, X. K., Zhang, Y. W., Sun, X. L., Yao, X. S., Tang, J. S.

Journal:

Eur J Med Chem (2018): 252-262

The cardenolides strophanthidin, digoxigenin and dihydroouabain act as activators of the human RORgamma/RORgammaT receptors

Authors:

Karas, K., Salkowska, A., Walczak-Drzewiecka, A., Ryba, K., Dastych, J., Bachorz, R. A., Ratajewski, M.

Journal:

Toxicol Lett (2018): 314-324

Physical properties

Molecular weight	485.62
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C)
UNSPSC	12352200

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Page updated on October 12, 2025