Calculator
Digoxigenin alkyne
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 485.62 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C) |
| UNSPSC | 12352200 |
Alternative formats
| Digoxigenin maleimide |
| Digoxigenin azide |
| Overview |  SDSProtocol |
See also: Click Chemistry
Molecular weight 485.62 |
Digoxigenin (DIG) is a hapten, a small molecule with high antigenicity, which is used in many biological detections similarly to other popular haptens such as 2,4-Dinitrophenol (DNP) and biotin. Typically, digoxigenin is conjugated to a macrobiomolecule (e.g., proteins, nucleic acids, etc) to be detected. Anti-digoxigenin antibodies are labeled with fluorescent dyes or enzymes, directly or secondarily, for visualization and detection. Digoxigenin alkyne readily reacts with azido group under mild conditions, attaching the digoxigenin moiety to azido-containing molecules. Digoxigenin-labled biomolecules are used for biological detections in combination with anti-digoxigenin antibodies of high affinity and specificity.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Digoxigenin alkyne to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 205.922 µL | 1.03 mL | 2.059 mL | 10.296 mL | 20.592 mL |
| 5 mM | 41.184 µL | 205.922 µL | 411.845 µL | 2.059 mL | 4.118 mL |
| 10 mM | 20.592 µL | 102.961 µL | 205.922 µL | 1.03 mL | 2.059 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| AMCA Alkyne | 346 | 434 | 19000 | 0.153 |
| XFD488 alkyne *Same Structure to Alexa Fluor™ 488 alkyne* | 499 | 520 | 71000 | 0.11 |
| ICG alkyne | 789 | 813 | 230000 | 0.076 |
| Cy3B alkyne | 560 | 571 | 1200001 | 0.069 |
| XFD647 Alkyne | 650 | 671 | 270000 | 0.03 |
Images
Figure 1. Chemical structure for Digoxigenin alkyne.
Citations
View all 21 citations: Citation Explorer
LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin
Authors: Ruppert, C., Phogat, N., Laufer, S., Kohl, M., Deigner, H. P.
Journal: Mikrochim Acta (2019): 119
Authors: Ruppert, C., Phogat, N., Laufer, S., Kohl, M., Deigner, H. P.
Journal: Mikrochim Acta (2019): 119
Application of Antibody-Powered Triplex-DNA Nanomachine to Electrochemiluminescence Biosensor for the Detection of Anti-Digoxigenin with Improved Sensitivity Versus Cycling Strand Displacement Reaction
Authors: Yang, S. S., Jiang, M. H., Chai, Y. Q., Yuan, R., Zhuo, Y.
Journal: ACS Appl Mater Interfaces (2018): 38648-38655
Authors: Yang, S. S., Jiang, M. H., Chai, Y. Q., Yuan, R., Zhuo, Y.
Journal: ACS Appl Mater Interfaces (2018): 38648-38655
Synthesis of C3-Neoglycosides of digoxigenin and their anticancer activities
Authors: Li, X. S., Ren, Y. C., Bao, Y. Z., Liu, J., Zhang, X. K., Zhang, Y. W., Sun, X. L., Yao, X. S., Tang, J. S.
Journal: Eur J Med Chem (2018): 252-262
Authors: Li, X. S., Ren, Y. C., Bao, Y. Z., Liu, J., Zhang, X. K., Zhang, Y. W., Sun, X. L., Yao, X. S., Tang, J. S.
Journal: Eur J Med Chem (2018): 252-262
The cardenolides strophanthidin, digoxigenin and dihydroouabain act as activators of the human RORgamma/RORgammaT receptors
Authors: Karas, K., Salkowska, A., Walczak-Drzewiecka, A., Ryba, K., Dastych, J., Bachorz, R. A., Ratajewski, M.
Journal: Toxicol Lett (2018): 314-324
Authors: Karas, K., Salkowska, A., Walczak-Drzewiecka, A., Ryba, K., Dastych, J., Bachorz, R. A., Ratajewski, M.
Journal: Toxicol Lett (2018): 314-324
Nonradioactive Detection of Small RNAs Using Digoxigenin-Labeled Probes
Authors: Tomassi, A. H., Gagliardi, D., Cambiagno, D. A., Manavella, P. A.
Journal: Methods Mol Biol (2017): 199-210
Authors: Tomassi, A. H., Gagliardi, D., Cambiagno, D. A., Manavella, P. A.
Journal: Methods Mol Biol (2017): 199-210
Synthesis of MeON-neoglycosides of digoxigenin with 6-deoxy- and 2,6-dideoxy-d-glucose derivatives and their anticancer activity
Authors: Wang, D. D., Li, X. S., Bao, Y. Z., Liu, J., Zhang, X. K., Yao, X. S., Sun, X. L., Tang, J. S.
Journal: Bioorg Med Chem Lett (2017): 3359-3364
Authors: Wang, D. D., Li, X. S., Bao, Y. Z., Liu, J., Zhang, X. K., Yao, X. S., Sun, X. L., Tang, J. S.
Journal: Bioorg Med Chem Lett (2017): 3359-3364
Generation of digoxigenin-incorporated probes to enhance DNA detection sensitivity
Authors: Lai, T. P., Wright, W. E., Shay, J. W.
Journal: Biotechniques (2016): 306-9
Authors: Lai, T. P., Wright, W. E., Shay, J. W.
Journal: Biotechniques (2016): 306-9
Simple and nonradioactive detection of microRNAs using digoxigenin (DIG)-labeled probes with high sensitivity
Authors: Wu, W., Gong, P., Li, J., Yang, J., Zhang, G., Li, H., Yang, Z., Zhang, X.
Journal: RNA (2014): 580-4
Authors: Wu, W., Gong, P., Li, J., Yang, J., Zhang, G., Li, H., Yang, Z., Zhang, X.
Journal: RNA (2014): 580-4
Elimination of nonspecific bands in non-radioactive electrophoretic mobility shift assays using the digoxigenin system
Authors: Tokunaga, S., Stegeman, J. J.
Journal: Anal Biochem (2014): 70-2
Authors: Tokunaga, S., Stegeman, J. J.
Journal: Anal Biochem (2014): 70-2