Calculator
Digoxigenin azide
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 516.64 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12352200 |
Alternative formats
| Digoxigenin maleimide |
| Digoxigenin alkyne |
| Overview |  SDSProtocol |
See also: Click Chemistry
Molecular weight 516.64 |
Digoxigenin (DIG) is a hapten, a small molecule with high antigenicity, which is used in many biological detections similarly to other popular haptens such as 2,4-Dinitrophenol (DNP) and biotin. Typically, digoxigenin is conjugated to a macrobiomolecule (e.g., proteins, nucleic acids, etc) to be detected. Anti-digoxigenin antibodies are labeled with fluorescent dyes or enzymes, directly or secondarily, for visualization and detection. Digoxigenin azide readily reacts with alkyne group under mild conditions, attaching the digoxigenin moiety to alkyne-containing molecules. Digoxigenin-labled biomolecules are used for biological detections in combination with anti-digoxigenin antibodies of high affinity and specificity.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of Digoxigenin azide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 193.558 µL | 967.792 µL | 1.936 mL | 9.678 mL | 19.356 mL |
| 5 mM | 38.712 µL | 193.558 µL | 387.117 µL | 1.936 mL | 3.871 mL |
| 10 mM | 19.356 µL | 96.779 µL | 193.558 µL | 967.792 µL | 1.936 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Correction Factor (280 nm) |
| AMCA Azide | 346 | 434 | 19000 | 0.153 |
| XFD488 azide *Same Structure to Alexa Fluor™ 488 azide* | 499 | 520 | 71000 | 0.11 |
| ICG azide | 789 | 813 | 230000 | 0.076 |
| Cy3B azide | 560 | 571 | 1200001 | 0.069 |
| XFD647 Azide | 650 | 671 | 270000 | 0.03 |
Images
Figure 1. Chemical structure for Digoxigenin azide.
Citations
View all 21 citations: Citation Explorer
LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin
Authors: Ruppert, C., Phogat, N., Laufer, S., Kohl, M., Deigner, H. P.
Journal: Mikrochim Acta (2019): 119
Authors: Ruppert, C., Phogat, N., Laufer, S., Kohl, M., Deigner, H. P.
Journal: Mikrochim Acta (2019): 119
Application of Antibody-Powered Triplex-DNA Nanomachine to Electrochemiluminescence Biosensor for the Detection of Anti-Digoxigenin with Improved Sensitivity Versus Cycling Strand Displacement Reaction
Authors: Yang, S. S., Jiang, M. H., Chai, Y. Q., Yuan, R., Zhuo, Y.
Journal: ACS Appl Mater Interfaces (2018): 38648-38655
Authors: Yang, S. S., Jiang, M. H., Chai, Y. Q., Yuan, R., Zhuo, Y.
Journal: ACS Appl Mater Interfaces (2018): 38648-38655
Synthesis of C3-Neoglycosides of digoxigenin and their anticancer activities
Authors: Li, X. S., Ren, Y. C., Bao, Y. Z., Liu, J., Zhang, X. K., Zhang, Y. W., Sun, X. L., Yao, X. S., Tang, J. S.
Journal: Eur J Med Chem (2018): 252-262
Authors: Li, X. S., Ren, Y. C., Bao, Y. Z., Liu, J., Zhang, X. K., Zhang, Y. W., Sun, X. L., Yao, X. S., Tang, J. S.
Journal: Eur J Med Chem (2018): 252-262
The cardenolides strophanthidin, digoxigenin and dihydroouabain act as activators of the human RORgamma/RORgammaT receptors
Authors: Karas, K., Salkowska, A., Walczak-Drzewiecka, A., Ryba, K., Dastych, J., Bachorz, R. A., Ratajewski, M.
Journal: Toxicol Lett (2018): 314-324
Authors: Karas, K., Salkowska, A., Walczak-Drzewiecka, A., Ryba, K., Dastych, J., Bachorz, R. A., Ratajewski, M.
Journal: Toxicol Lett (2018): 314-324
Nonradioactive Detection of Small RNAs Using Digoxigenin-Labeled Probes
Authors: Tomassi, A. H., Gagliardi, D., Cambiagno, D. A., Manavella, P. A.
Journal: Methods Mol Biol (2017): 199-210
Authors: Tomassi, A. H., Gagliardi, D., Cambiagno, D. A., Manavella, P. A.
Journal: Methods Mol Biol (2017): 199-210
Synthesis of MeON-neoglycosides of digoxigenin with 6-deoxy- and 2,6-dideoxy-d-glucose derivatives and their anticancer activity
Authors: Wang, D. D., Li, X. S., Bao, Y. Z., Liu, J., Zhang, X. K., Yao, X. S., Sun, X. L., Tang, J. S.
Journal: Bioorg Med Chem Lett (2017): 3359-3364
Authors: Wang, D. D., Li, X. S., Bao, Y. Z., Liu, J., Zhang, X. K., Yao, X. S., Sun, X. L., Tang, J. S.
Journal: Bioorg Med Chem Lett (2017): 3359-3364
Generation of digoxigenin-incorporated probes to enhance DNA detection sensitivity
Authors: Lai, T. P., Wright, W. E., Shay, J. W.
Journal: Biotechniques (2016): 306-9
Authors: Lai, T. P., Wright, W. E., Shay, J. W.
Journal: Biotechniques (2016): 306-9
Simple and nonradioactive detection of microRNAs using digoxigenin (DIG)-labeled probes with high sensitivity
Authors: Wu, W., Gong, P., Li, J., Yang, J., Zhang, G., Li, H., Yang, Z., Zhang, X.
Journal: RNA (2014): 580-4
Authors: Wu, W., Gong, P., Li, J., Yang, J., Zhang, G., Li, H., Yang, Z., Zhang, X.
Journal: RNA (2014): 580-4
Elimination of nonspecific bands in non-radioactive electrophoretic mobility shift assays using the digoxigenin system
Authors: Tokunaga, S., Stegeman, J. J.
Journal: Anal Biochem (2014): 70-2
Authors: Tokunaga, S., Stegeman, J. J.
Journal: Anal Biochem (2014): 70-2