Digoxigenin C9 acid

Digoxigenin (DIG) is a hapten, a small molecule with high antigenicity, which is used in many biological detections similarly to other popular haptens such as 2,4-Dinitrophenol (DNP) and biotin. Typically, digoxigenin is conjugated to a macrobiomolecule (e.g., proteins, nucleic acids, etc) to be detected. Anti-digoxigenin antibodies are labeled with fluorescent dyes or enzymes, directly or secondarily, for visualization and detection. Digoxigenin C9 acid is an affordable building block which can be used for attaching the digoxigenin moiety to peptides, proteins, amino-substituted oligonucleotides or other biological molecules. Digoxigenin-labeled biomolecules are used for biological detections in combination with anti-digoxigenin antibodies of high affinity and specificity.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
3510	5 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Digoxigenin C9 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	178.025 µL	890.123 µL	1.78 mL	8.901 mL	17.802 mL
5 mM	35.605 µL	178.025 µL	356.049 µL	1.78 mL	3.56 mL
10 mM	17.802 µL	89.012 µL	178.025 µL	890.123 µL	1.78 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	561.72 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Digoxigenin.

Authors:

Green, Michael R and Sambrook, Joseph

Journal:

Cold Spring Harbor protocols (2022): pdb.top100784

Production of Digoxigenin-Labeled Riboprobes for In Situ Hybridization Experiments.

Authors:

Barratt, Kristen S and Arkell, Ruth M

Journal:

Current protocols in mouse biology (2020): e74

A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin.

Authors:

Ruppert, Christoph and Phogat, Navneet and Laufer, Stefan and Kohl, Matthias and Deigner, Hans-Peter

Journal:

Mikrochimica acta (2019): 119

The cardenolides strophanthidin, digoxigenin and dihydroouabain act as activators of the human RORγ/RORγT receptors.

Authors:

Karaś, Kaja and Sałkowska, Anna and Walczak-Drzewiecka, Aurelia and Ryba, Katarzyna and Dastych, Jarosław and Bachorz, Rafał A and Ratajewski, Marcin

Journal:

Toxicology letters (2018): 314-324

Synthesis of C3-Neoglycosides of digoxigenin and their anticancer activities.

Authors:

Li, Xiao-San and Ren, Yi-Chang and Bao, Yu-Zhou and Liu, Jie and Zhang, Xiao-Kun and Zhang, You-Wei and Sun, Xue-Long and Yao, Xin-Sheng and Tang, Jin-Shan

Journal:

European journal of medicinal chemistry (2018): 252-262

Physical properties

Molecular weight	561.72
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on November 20, 2025