Name: Digoxigenin maleimide
Brand: AAT Bioquest
SKU: 3503
Price: 232 USD
Availability: InStock

Digoxigenin maleimide

Digoxigenin (DIG) is a hapten, a small molecule with high antigenicity, which is used in many biological detections similarly to other popular haptens such as 2,4-Dinitrophenol (DNP) and biotin. Typically, digoxigenin is conjugated to a macrobiomolecule (e.g., proteins, nucleic acids, etc) to be detected. Anti-digoxigenin antibodies are labeled with fluorescent dyes or enzymes, directly or secondarily, for visualization and detection. Digoxigenin maleimide readily reacts with thiol group under mild conditions, attaching the digoxigenin moiety to proteins or thiol-substituted oligonucleotides. Digoxigenin-labled biomolecules are used for biological detections in combination with anti-digoxigenin antibodies of high affinity and specificity.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
3503	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Digoxigenin maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	175.23 µL	876.148 µL	1.752 mL	8.761 mL	17.523 mL
5 mM	35.046 µL	175.23 µL	350.459 µL	1.752 mL	3.505 mL
10 mM	17.523 µL	87.615 µL	175.23 µL	876.148 µL	1.752 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	570.68 g/mol	=	mL	x	mM	=	mmol

Citations

View all 20 citations: Citation Explorer

A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin

Authors:

Ruppert, C., Phogat, N., Laufer, S., Kohl, M., Deigner, H. P.

Journal:

Mikrochim Acta (2019): 119

Application of Antibody-Powered Triplex-DNA Nanomachine to Electrochemiluminescence Biosensor for the Detection of Anti-Digoxigenin with Improved Sensitivity Versus Cycling Strand Displacement Reaction

Authors:

Yang, S. S., Jiang, M. H., Chai, Y. Q., Yuan, R., Zhuo, Y.

Journal:

ACS Appl Mater Interfaces (2018): 38648-38655

Synthesis of C3-Neoglycosides of digoxigenin and their anticancer activities

Authors:

Li, X. S., Ren, Y. C., Bao, Y. Z., Liu, J., Zhang, X. K., Zhang, Y. W., Sun, X. L., Yao, X. S., Tang, J. S.

Journal:

Eur J Med Chem (2018): 252-262

The cardenolides strophanthidin, digoxigenin and dihydroouabain act as activators of the human RORgamma/RORgammaT receptors

Authors:

Karas, K., Salkowska, A., Walczak-Drzewiecka, A., Ryba, K., Dastych, J., Bachorz, R. A., Ratajewski, M.

Journal:

Toxicol Lett (2018): 314-324

Nonradioactive Detection of Small RNAs Using Digoxigenin-Labeled Probes

Authors:

Tomassi, A. H., Gagliardi, D., Cambiagno, D. A., Manavella, P. A.

Journal:

Methods Mol Biol (2017): 199-210

Physical properties

Molecular weight	570.68
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C)
UNSPSC	12352200

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Page updated on October 13, 2025