Digoxigenin NHS ester

Digoxigenin (DIG) is a hapten, a small molecule with high antigenicity, which is used in many biological detections similarly to other popular haptens such as 2,4-Dinitrophenol (DNP) and biotin. Typically, digoxigenin is conjugated to a macrobiomolecule (e.g., proteins, nucleic acids, etc) to be detected. Anti-digoxigenin antibodies are labeled with fluorescent dyes or enzymes, directly or secondarily, for visualization and detection. Digoxigenin NHS ester is an activated ester which readily reacts with amino groups under mild conditions, attaching the digoxigenin moiety to proteins or amino-substituted oligonucleotides. Digoxigenin-labled biomolecules are used for biological detections in combination with anti-digoxigenin antibodies of high affinity and specificity.

Protocol

COO

COA

Catalog	Size	Price	Quantity
3500	1 mg	Price
3501	5 mg	Price

Citations

View all 21 citations: Citation Explorer

LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis

Authors:

Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua

Journal:

Biochemical and Biophysical Research Communications (2021): 95--102

A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin

Authors:

Ruppert, C., Phogat, N., Laufer, S., Kohl, M., Deigner, H. P.

Journal:

Mikrochim Acta (2019): 119

Application of Antibody-Powered Triplex-DNA Nanomachine to Electrochemiluminescence Biosensor for the Detection of Anti-Digoxigenin with Improved Sensitivity Versus Cycling Strand Displacement Reaction

Authors:

Yang, S. S., Jiang, M. H., Chai, Y. Q., Yuan, R., Zhuo, Y.

Journal:

ACS Appl Mater Interfaces (2018): 38648-38655

Synthesis of C3-Neoglycosides of digoxigenin and their anticancer activities

Authors:

Li, X. S., Ren, Y. C., Bao, Y. Z., Liu, J., Zhang, X. K., Zhang, Y. W., Sun, X. L., Yao, X. S., Tang, J. S.

Journal:

Eur J Med Chem (2018): 252-262

The cardenolides strophanthidin, digoxigenin and dihydroouabain act as activators of the human RORgamma/RORgammaT receptors

Authors:

Karas, K., Salkowska, A., Walczak-Drzewiecka, A., Ryba, K., Dastych, J., Bachorz, R. A., Ratajewski, M.

Journal:

Toxicol Lett (2018): 314-324

Physical properties

Molecular weight	658.79
Solvent	DMSO

Storage, safety and handling

Certificate of Origin	Download PDF
H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	129273-26-3

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Page updated on October 13, 2025