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Digoxigenin NHS ester

Digoxigenin (DIG) is a hapten, a small molecule with high antigenicity, which is used in many biological detections similarly to other popular haptens such as 2,4-Dinitrophenol (DNP) and biotin. Typically, digoxigenin is conjugated to a macrobiomolecule (e.g., proteins, nucleic acids, etc) to be detected. Anti-digoxigenin antibodies are labeled with fluorescent dyes or enzymes, directly or secondarily, for visualization and detection. Digoxigenin NHS ester is an activated ester which readily reacts with amino groups under mild conditions, attaching the digoxigenin moiety to proteins or amino-substituted oligonucleotides. Digoxigenin-labled biomolecules are used for biological detections in combination with anti-digoxigenin antibodies of high affinity and specificity.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Digoxigenin NHS ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM151.793 µL758.967 µL1.518 mL7.59 mL15.179 mL
5 mM30.359 µL151.793 µL303.587 µL1.518 mL3.036 mL
10 mM15.179 µL75.897 µL151.793 µL758.967 µL1.518 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (260 nm)Correction Factor (280 nm)
XFD488 NHS Ester *Same Structure to Alexa Fluor™ 488 NHS Ester*499520710000.9210.300.11
XFD350 NHS Ester *Same Structure to Alexa Fluor™ 350 NHS Ester*34344119000-0.250.19
XFD532 NHS Ester *Same Structure to Alexa Fluor™ 532 NHS Ester*534553810000.6110.240.09
XFD594 NHS Ester *Same Structure to Alexa Fluor™ 594 NHS Ester*590618900000.6610.430.56
QXY21 NHS ester [equivalent to QSY-21 NHS ester]--890001--0.32
XFD555 NHS Ester *Same Structure to Alexa Fluor™ 555 NHS Ester*5535681500000.110.080.08
XFD647 NHS Ester *Same Structure to Alexa Fluor™ 647 NHS Ester*6506712390000.3310.000.03
XFD680 NHS Ester *Same Structure to Alexa Fluor™ 680 NHS Ester*6817041840000.3610.000.05
XFD700 NHS Ester *Same Structure to Alexa Fluor™ 700 NHS Ester*6967191920000.2510.000.07
XFD750 NHS Ester *Same Structure to Alexa Fluor™ 750 NHS Ester*7527762400000.1210.000.04
Cy5DIGE NHS ester65167025000010.271, 0.420.020.03
Cy2DIGE NHS ester4925081500000.1200.080.15
Cy3DIGE NHS ester55556915000010.1510.070.073
XFD546 NHS Ester *Same Structure to Alexa Fluor™ 546 NHS Ester*5615721120000.7910.210.12
XFD568 NHS Ester *Same Structure to Alexa Fluor™ 568 NHS Ester*579603913000.6910.450.46
XFD514 NHS Ester *Same Structure to Alexa Fluor™ 514 NHS Ester*51854380000-0.310.18
QXY7 NHS ester [equivalent to QSY-7 NHS ester]--900001--0.22
Cy3B NHS ester56057112000010.5810.0480.069
XFD405 NHS Ester [equivalent to Alexa Fluor™ 405 NHS Ester]40142135,000-0.230.70
CypHer5E NHS Ester643660----
CypHer7E NHS Ester748769----
XFD660 NHS Ester [equivalent to Alexa Fluor® 660 NHS Ester]663691132000-0.000.10
XFD430 NHS ester43254015,000--0.28
Show More (14)

Citations

View all 21 citations: Citation Explorer
LncRNA AL592284. 1 facilitates proliferation and metastasis of cervical cancer cells via miR-30a-5p/Vimentin/EMT axis
Authors: Zhang, Jing and Liu, Hong-li and Liu, Jing-bo and Zhang, Yuan and Liu, Jing and Li, Yan-hua
Journal: Biochemical and Biophysical Research Communications (2021): 95--102
A smartphone readout system for gold nanoparticle-based lateral flow assays: application to monitoring of digoxigenin
Authors: Ruppert, C., Phogat, N., Laufer, S., Kohl, M., Deigner, H. P.
Journal: Mikrochim Acta (2019): 119
Application of Antibody-Powered Triplex-DNA Nanomachine to Electrochemiluminescence Biosensor for the Detection of Anti-Digoxigenin with Improved Sensitivity Versus Cycling Strand Displacement Reaction
Authors: Yang, S. S., Jiang, M. H., Chai, Y. Q., Yuan, R., Zhuo, Y.
Journal: ACS Appl Mater Interfaces (2018): 38648-38655
Synthesis of C3-Neoglycosides of digoxigenin and their anticancer activities
Authors: Li, X. S., Ren, Y. C., Bao, Y. Z., Liu, J., Zhang, X. K., Zhang, Y. W., Sun, X. L., Yao, X. S., Tang, J. S.
Journal: Eur J Med Chem (2018): 252-262
The cardenolides strophanthidin, digoxigenin and dihydroouabain act as activators of the human RORgamma/RORgammaT receptors
Authors: Karas, K., Salkowska, A., Walczak-Drzewiecka, A., Ryba, K., Dastych, J., Bachorz, R. A., Ratajewski, M.
Journal: Toxicol Lett (2018): 314-324
Page updated on November 7, 2024

Ordering information

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Unit size
1 mg
5 mg
Catalog Number
35003501
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Physical properties

Molecular weight

658.79

Solvent

DMSO

Storage, safety and handling

Certificate of OriginDownload PDF
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12352200

CAS

129273-26-3
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