Dihydrofluorescein diacetate [Fluorescin diacetate] CAS 35340-49-9

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Physical properties

Molecular weight	418.4
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200

Overview SDS Protocol

CAS

35340-49-9

Molecular weight

418.4

Absorbance (nm)

487

Correction Factor (260 nm)

0.32

Correction Factor (280 nm)

0.35

Extinction coefficient (cm ^-1 M ^-1)

80000¹

Excitation (nm)

498

Emission (nm)

517

Quantum yield

0.7900¹, 0.95²

Dihydrofluorescein diacetate (also called fluorescin diacetate) is hydrolyzed by cellular esterases to dihydrofluorescein (also called fluorescin) and is then oxidized to fluorescein primarily by H2O2. Dihydrofluorescein diacetate might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Cell-loading studies indicate that dihydrofluorescein achieves higher intracellular concentrations than the other redox sensors such as 2',7'-dichlorodihydrofluorescein and Dihydrorhodamine 123.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Dihydrofluorescein diacetate [Fluorescin diacetate] *CAS 35340-49-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	239.006 µL	1.195 mL	2.39 mL	11.95 mL	23.901 mL
5 mM	47.801 µL	239.006 µL	478.011 µL	2.39 mL	4.78 mL
10 mM	23.901 µL	119.503 µL	239.006 µL	1.195 mL	2.39 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Absorbance (nm)	487
Correction Factor (260 nm)	0.32
Correction Factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900¹, 0.95²

Images

Figure 1. Chemical structure for Dihydrofluorescein diacetate [Fluorescin diacetate] *CAS 35340-49-9*

References

View all 11 references: Citation Explorer

Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes
Authors: Diaz G, Liu S, Isola R, Diana A, Falchi AM.
Journal: Histochem Cell Biol (2003): 319

Kinetic analysis of fluorescein and dihydrofluorescein effluxes in tumour cells expressing the multidrug resistance protein, MRP1
Authors: Saengkhae C, Loetchutinat C, Garnier-Suillerot A.
Journal: Biochem Pharmacol (2003): 969

Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123
Authors: Hempel SL, Buettner GR, O'Malley YQ, Wessels DA, Flaherty DM.
Journal: Free Radic Biol Med (1999): 146

A rapid diagnostic test for the viability of early cattle and rabbit embryos using diacetyl-fluorescin
Authors: Schilling E, Dopke HH.
Journal: Naturwissenschaften (1978): 658

Determination of fluorescin sodium in ophthalmic solutions
Authors: Robertson EJ, Patel JA.
Journal: Am J Hosp Pharm (1968): 598

Experimental research on insulin antibodies. III. Immunohistology of the endocrine pancreas with guinea pig anti-bovine insulin serum, conjugated with fluorescin isothiocyanate.
Authors: Costanzi G, Mancini UM, Zampa GA.
Journal: Boll Soc Ital Biol Sper (1961): 1233

Experimental research on insulin antibodies. II. Immunochemical characterization of guinea pig anti-ox insulin serum conjugated with fluorescin isothiocyanate.
Authors: Mancini AM, Zampa GA, Costanzi G.
Journal: Boll Soc Ital Biol Sper (1961): 1230

Two simple media for the demonstration of pyocyanin and fluorescin
Authors: King EO, Ward MK, Raney DE.
Journal: J Lab Clin Med (1954): 301

Influence of the concentration of iron on the production of fluorescin by Pseudomonas aeruginosa
Authors: Totter JR, Moseley FT.
Journal: J Bacteriol (1953): 45

Determination of circulation time with fluorescin in normal children and in congenital or acquired cardiopathy.
Authors: Bulgarelli R, Garioni F.
Journal: Minerva Pediatr (1952): 576