Name: Dihydrofluorescein diacetate [Fluorescin diacetate] *CAS 35340-49-9*
Brand: AAT Bioquest
SKU: 15203
Price: 90 USD
Availability: InStock

Dihydrofluorescein diacetate

Fluorescin diacetate; CAS 35340-49-9

Dihydrofluorescein diacetate (also called fluorescin diacetate) is hydrolyzed by cellular esterases to dihydrofluorescein (also called fluorescin) and is then oxidized to fluorescein primarily by H2O2. Dihydrofluorescein diacetate might be reactive toward a broad range of oxidizing reactions that may be increased during intracellular oxidant stress. Cell-loading studies indicate that dihydrofluorescein achieves higher intracellular concentrations than the other redox sensors such as 2',7'-dichlorodihydrofluorescein and Dihydrorhodamine 123.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
15203	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of Dihydrofluorescein diacetate [Fluorescin diacetate] *CAS 35340-49-9* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	239.006 µL	1.195 mL	2.39 mL	11.95 mL	23.901 mL
5 mM	47.801 µL	239.006 µL	478.011 µL	2.39 mL	4.78 mL
10 mM	23.901 µL	119.503 µL	239.006 µL	1.195 mL	2.39 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	418.4 g/mol	=	mL	x	mM	=	mmol

References

View all 11 references: Citation Explorer

Kinetic analysis of fluorescein and dihydrofluorescein effluxes in tumour cells expressing the multidrug resistance protein, MRP1

Authors:

Saengkhae C, Loetchutinat C, Garnier-Suillerot A.

Journal:

Biochem Pharmacol (2003): 969

Mitochondrial localization of reactive oxygen species by dihydrofluorescein probes

Authors:

Diaz G, Liu S, Isola R, Diana A, Falchi AM.

Journal:

Histochem Cell Biol (2003): 319

Dihydrofluorescein diacetate is superior for detecting intracellular oxidants: comparison with 2',7'-dichlorodihydrofluorescein diacetate, 5(and 6)-carboxy-2',7'-dichlorodihydrofluorescein diacetate, and dihydrorhodamine 123

Authors:

Hempel SL, Buettner GR, O'Malley YQ, Wessels DA, Flaherty DM.

Journal:

Free Radic Biol Med (1999): 146

A rapid diagnostic test for the viability of early cattle and rabbit embryos using diacetyl-fluorescin

Authors:

Schilling E, Dopke HH.

Journal:

Naturwissenschaften (1978): 658

Determination of fluorescin sodium in ophthalmic solutions

Authors:

Robertson EJ, Patel JA.

Journal:

Am J Hosp Pharm (1968): 598

Physical properties

Molecular weight	418.4
Solvent	DMSO

Spectral properties

Absorbance (nm)	487
Correction factor (260 nm)	0.32
Correction factor (280 nm)	0.35
Extinction coefficient (cm ^-1 M ^-1)	80000 ¹
Excitation (nm)	498
Emission (nm)	517
Quantum yield	0.7900 ¹ , 0.95 ²

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	35340-49-9

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Page updated on October 13, 2025