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DiIC18(3)-DS [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine-5,5-disulfonic acid]

Chemical structure for DiIC18(3)-DS [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine-5,5-disulfonic acid]
Chemical structure for DiIC18(3)-DS [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine-5,5-disulfonic acid]
Ordering information
Price ()
Catalog Number22052
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight993.53
SolventDMSO
Spectral properties
Extinction coefficient (cm -1 M -1)148000
Excitation (nm)550
Emission (nm)564
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


Molecular weight
993.53
Extinction coefficient (cm -1 M -1)
148000
Excitation (nm)
550
Emission (nm)
564
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range. This particular DiI derivative is an amphiphilic molecule that contains both hydrophilic and lipophilic moiety. It has stronger fluorescence in water than the highly lipophilic DiI analogs.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiIC18(3)-DS [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine-5,5-disulfonic acid] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM100.651 µL503.256 µL1.007 mL5.033 mL10.065 mL
5 mM20.13 µL100.651 µL201.302 µL1.007 mL2.013 mL
10 mM10.065 µL50.326 µL100.651 µL503.256 µL1.007 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)148000
Excitation (nm)550
Emission (nm)564

Citations


View all 44 citations: Citation Explorer
Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure
Authors: Wang, Kun and Li, Yuwen and Zhu, Tiantian and Zhang, Yongting and Li, Wenting and Lin, Wenyu and Li, Jun and Zhu, Chuanlong
Journal: Stem Cell Research & Therapy (2017): 162
The accelerated blood clearance phenomenon of PEGylated nanoemulsion upon cross administration with nanoemulsions modified with polyglycerin
Authors: Su, Yuqing and Wang, Lirong and Liang, Kaifan and Liu, Mengyang and Liu, Xinrong and Song, Yanzhi and Deng, Yihui
Journal: Asian Journal of Pharmaceutical Sciences (2017)
In vivo imaging system for explants analysis–A new approach for assessment of cell transplantation effects in large animal models
Authors: Zarychta-Wisniewska, Weronika and Burdzinska, Anna and Zagozdzon, Radoslaw and Dybowski, Bartosz and Butrym, Marta and Gajewski, Zdzislaw and Paczek, Leszek
Journal: PloS one (2017): e0184588
On-Demand Drug Releasing from Dual Targeting Small Nanoparticles Triggered by High Intensity Focused Ultrasound Enhanced Glioblastoma Targeting Therapy
Authors: Luo, Zimiao and Jin, Kai and Pang, Qiang and shen, shun and Yan, Zhiqiang and Jiang, Ting and Zhu, Xiaoyan and Yu, Lei and Pang, Zhiqing and Jiang, Xinguo
Journal: ACS Applied Materials & Interfaces (2017)
Cyclic RGD peptide-modified liposomal drug delivery system for targeted oral apatinib administration: enhanced cellular uptake and improved therapeutic effects
Authors: Song, Zhiwang and Lin, Yun and Xia Zhang, Chan Feng and Lu, Yonglin and Gao, Yong and Dong, Chunyan
Journal: International Journal of Nanomedicine (2017): 1941
Novel approach for the detection of intraperitoneal micrometastasis using an ovarian cancer mouse model
Authors: Alvero, Ayesha B and Kim, Dongin and Lima, Eydis and Sumi, Natalia J and Lee, Jung Seok and Cardenas, Carlos and Pitruzzello, Mary and Silasi, Dan-Arin and Buza, Natalia and Fahmy, Tarek and others, undefined
Journal: Scientific Reports (2017)
Mesenchymal stem cells increase skin graft survival time and up-regulate PD-L1 expression in splenocytes of mice
Authors: Moravej, Ali and Geramizadeh, Bita and Azarpira, Negar and Zarnani, Amir-Hasan and Yaghobi, Ramin and Kalani, Mehdi and Khosravi, Maryam and Kouhpayeh, Amin and Karimi, Mohammad-Hossein
Journal: Immunology Letters (2017)
Influence of Particle Geometry on Gastrointestinal Transit and Absorption following Oral Administration
Authors: Li, Dong and Zhuang, Jie and He, Haisheng and Jiang, Sifan and Banerjee, Amrita and Lu, Yi and Wu, Wei and Mitragotri, Samir and Gan, Li and Qi, Jianping
Journal: ACS Applied Materials & Interfaces (2017)
cRGD-functionalized reduction-sensitive shell-sheddable biodegradable micelles mediate enhanced doxorubicin delivery to human glioma xenografts in vivo
Authors: Zhu, Yaqin and Zhang, Jian and Meng, Fenghua and Deng, Chao and Cheng, Ru and Feijen, Jan and Zhong, Zhiyuan
Journal: Journal of Controlled Release (2016): 29--38
Annonaceous acetogenins nanosuspensions stabilized by PCL-PEG block polymer: significantly improved antitumor efficacy
Authors: Hong, Jingyi and Li, Yanhong and Li, Yijing and Xiao, Yao and Kuang, Haixue and Wang, Xiangtao
Journal: International Journal of Nanomedicine (2016): 3239

References


View all 92 references: Citation Explorer
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Functional neuroanatomy of the rhinophore of Aplysia punctata
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Regional cardiac ganglia projections in the guinea pig heart studied by postmortem DiI tracing
Authors: Harrison TA, Perry KM, Hoover DB.
Journal: Anat Rec A Discov Mol Cell Evol Biol (2005): 758
Afferent and efferent connections of the cerebellum of the chondrostean Acipenser baeri: a carbocyanine dye (DiI) tracing study
Authors: Huesa G, Anadon R, Yanez J.
Journal: J Comp Neurol (2003): 327
A novel morphological technique to investigate a single climbing fibre synaptogenesis with a Purkinje cell in the developing mouse cerebellum: DiI injection into the inferior cerebellar peduncle
Authors: Kiyohara Y, Endo K, Ide C, Mizoguchi A.
Journal: J Electron Microsc (Tokyo) (2003): 327
Retinal ganglion cells resistant to advanced glaucoma: a postmortem study of human retinas with the carbocyanine dye DiI
Authors: Pavlidis M, Stupp T, Naskar R, Cengiz C, Thanos S.
Journal: Invest Ophthalmol Vis Sci (2003): 5196
Projection pattern of nerve fibers from the septal organ: DiI-tracing studies with transgenic OMP mice
Authors: Levai O, Strotmann J.
Journal: Histochem Cell Biol (2003): 483