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DiO perchlorate [3,3-Dioctadecyloxacarbocyanine perchlorate] *CAS#: 34215-57-1*

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Physical properties
Molecular weight881.7
SolventDMSO
Spectral properties
Excitation (nm)483
Emission (nm)501
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200

OverviewpdfSDSpdfProtocol


CAS
34215-57-1
Molecular weight
881.7
Excitation (nm)
483
Emission (nm)
501
DiI, DiO, DiD and DiR dyes are a family of lipophilic fluorescent stains for labeling membranes and other hydrophobic structures. The fluorescence of these environment-sensitive dyes is greatly enhanced when incorporated into membranes or bound to lipophilic biomolecules such as proteins although they are weakly fluorescent in water. They have high extinction coefficients, polarity-dependent fluorescence and short excited-state lifetimes. Once applied to cells, these dyes diffuse laterally within the cellular plasma membranes, resulting in even staining of the entire cell at their optimal concentrations. The distinct fluorescence colors of DiI (orange fluorescence), DiO (green fluorescence), DiD (red fluorescence) and DiR (deep red fluorescent) provide a convenient tool for multicolor imaging and flow cytometric analysis of live cells. DiO and DiI can be used with standard FITC and TRITC filters respectively. Among them DiD is well excited by the 633 nm He-Ne laser, and has much longer excitation and emission wavelengths than those of DiI, providing a valuable alternative for labeling cells and tissues that have significant intrinsic fluorescence. DiR might be useful for in vivo imaging or tracing due to the effective transmission of infrared light through cells and tissues and low level of autofluorescence in the infrared range.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiO perchlorate [3,3-Dioctadecyloxacarbocyanine perchlorate] *CAS#: 34215-57-1* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM113.417 µL567.086 µL1.134 mL5.671 mL11.342 mL
5 mM22.683 µL113.417 µL226.835 µL1.134 mL2.268 mL
10 mM11.342 µL56.709 µL113.417 µL567.086 µL1.134 mL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Excitation (nm)483
Emission (nm)501

Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)
DiD Perchlorate *UltraPure Grade*646663260000
DiI perchlorate [1,1-Dioctadecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate] *CAS 41085-99-8*550564148000

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Citations


View all 49 citations: Citation Explorer
Exosomes Secreted by CSFV-Infected Cells Evade Neutralizing Antibody to Activate Innate Immune Responses and Establish Productive Infection in Recipient Cells
Authors: Bao, Xi and Zhuang, Tenhan and Xu, Yue and Chen, Li and Feng, Lei and Yao, Huochun
Journal: Veterinary Microbiology (2024): 110062
Bioactive cytomembrane@ poly (citrate-peptide)-miRNA365 nanoplatform with immune escape and homologous targeting for colon cancer therapy
Authors: Zhang, Long and Zhang, Wan and Peng, Hang and Shen, Tianli and Wang, Min and Luo, Meng and Qu, Xiaoyan and Qu, Fengyi and Liu, Wenguang and Lei, Bo and others,
Journal: Materials Today Bio (2022): 100294
Glomerular Signals Underlying Olfactory Navigation
Authors: Nouboussi Nkenfack, Nelly Merveille
Journal: (2020)
Design of Hydrolytically Degradable Polyethylene Glycol Crosslinkers for Facile Control of Hydrogel Degradation
Authors: Kroger, Stephanie M and Hill, Lindsay and Jain, Era and Stock, Aaron and Bracher, Paul J and He, Fahu and Zustiak, Silviya P
Journal: Macromolecular Bioscience (2020): 2000085
Myosin VII, USH1C, and ANKS4B or USH1G Together Form Condensed Molecular Assembly via Liquid-Liquid Phase Separation
Authors: He, Yunyun and Li, Jianchao and Zhang, Mingjie
Journal: Cell reports (2019): 974--986
Overexpression of c-Met in bone marrow mesenchymal stem cells improves their effectiveness in homing and repair of acute liver failure
Authors: Wang, Kun and Li, Yuwen and Zhu, Tiantian and Zhang, Yongting and Li, Wenting and Lin, Wenyu and Li, Jun and Zhu, Chuanlong
Journal: Stem Cell Research &amp; Therapy (2017): 162
The accelerated blood clearance phenomenon of PEGylated nanoemulsion upon cross administration with nanoemulsions modified with polyglycerin
Authors: Su, Yuqing and Wang, Lirong and Liang, Kaifan and Liu, Mengyang and Liu, Xinrong and Song, Yanzhi and Deng, Yihui
Journal: Asian Journal of Pharmaceutical Sciences (2017)
In vivo imaging system for explants analysis&ndash;A new approach for assessment of cell transplantation effects in large animal models
Authors: Zarychta-Wisniewska, Weronika and Burdzinska, Anna and Zagozdzon, Radoslaw and Dybowski, Bartosz and Butrym, Marta and Gajewski, Zdzislaw and Paczek, Leszek
Journal: PloS one (2017): e0184588
On-Demand Drug Releasing from Dual Targeting Small Nanoparticles Triggered by High Intensity Focused Ultrasound Enhanced Glioblastoma Targeting Therapy
Authors: Luo, Zimiao and Jin, Kai and Pang, Qiang and shen, shun and Yan, Zhiqiang and Jiang, Ting and Zhu, Xiaoyan and Yu, Lei and Pang, Zhiqing and Jiang, Xinguo
Journal: ACS Applied Materials &amp; Interfaces (2017)
Cyclic RGD peptide-modified liposomal drug delivery system for targeted oral apatinib administration: enhanced cellular uptake and improved therapeutic effects
Authors: Song, Zhiwang and Lin, Yun and Xia Zhang, Chan Feng and Lu, Yonglin and Gao, Yong and Dong, Chunyan
Journal: International Journal of Nanomedicine (2017): 1941

References


View all 92 references: Citation Explorer
Use of lipophilic near-infrared dye in whole-body optical imaging of hematopoietic cell homing
Authors: Kalchenko V, Shivtiel S, Malina V, Lapid K, Haramati S, Lapidot T, Brill A, Harmelin A.
Journal: J Biomed Opt (2006): 50507
Nox2, Ca2+, and protein kinase C play a role in angiotensin II-induced free radical production in nucleus tractus solitarius
Authors: Wang G, Anrather J, Glass MJ, Tarsitano MJ, Zhou P, Frys KA, Pickel VM, Iadecola C.
Journal: Hypertension (2006): 482
Functional neuroanatomy of the rhinophore of Aplysia punctata
Authors: Wertz A, Rossler W, Obermayer M, Bickmeyer U.
Journal: Front Zool (2006): 6
In vivo imaging and counting of rat retinal ganglion cells using a scanning laser ophthalmoscope
Authors: Higashide T, Kawaguchi I, Ohkubo S, Takeda H, Sugiyama K.
Journal: Invest Ophthalmol Vis Sci (2006): 2943
Confocal laser scanning microscopy using dialkylcarbocyanine dyes for cell tracing in hard and soft biomaterials
Authors: Heinrich L, Freyria AM, Melin M, Tourneur Y, Maksoud R, Bernengo JC, Hartmann DJ.
Journal: J Biomed Mater Res B Appl Biomater. (2006)
Regional cardiac ganglia projections in the guinea pig heart studied by postmortem DiI tracing
Authors: Harrison TA, Perry KM, Hoover DB.
Journal: Anat Rec A Discov Mol Cell Evol Biol (2005): 758
Afferent and efferent connections of the cerebellum of the chondrostean Acipenser baeri: a carbocyanine dye (DiI) tracing study
Authors: Huesa G, Anadon R, Yanez J.
Journal: J Comp Neurol (2003): 327
A novel morphological technique to investigate a single climbing fibre synaptogenesis with a Purkinje cell in the developing mouse cerebellum: DiI injection into the inferior cerebellar peduncle
Authors: Kiyohara Y, Endo K, Ide C, Mizoguchi A.
Journal: J Electron Microsc (Tokyo) (2003): 327
Retinal ganglion cells resistant to advanced glaucoma: a postmortem study of human retinas with the carbocyanine dye DiI
Authors: Pavlidis M, Stupp T, Naskar R, Cengiz C, Thanos S.
Journal: Invest Ophthalmol Vis Sci (2003): 5196
Projection pattern of nerve fibers from the septal organ: DiI-tracing studies with transgenic OMP mice
Authors: Levai O, Strotmann J.
Journal: Histochem Cell Biol (2003): 483