DiTO™-1 [equivalent to TOTO®-1] 5 mM DMSO Solution CAS 143413-84-7

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Physical properties

Molecular weight	1302.78
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	117000¹
Excitation (nm)	514
Emission (nm)	531
Quantum yield	0.34¹

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Overview SDS Protocol

CAS

143413-84-7

Molecular weight

1302.78

Extinction coefficient (cm ^-1 M ^-1)

117000¹

Excitation (nm)

514

Emission (nm)

531

Quantum yield

0.34¹

DiTO™-1 is chemically equivalent to TOTO®-1 (TOTO® is the trademark of Invitrogen). DiTO™-1 is a carbocyanine dimer with green fluorescence similar to FITC. It is cell-impermeant and easily distinguished from Cy5 and rhodamines as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiTO™-1 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Red™ LCS1 dye and cell-impermeant TOTO®-1 can be used to assess cell viability. DiTO™-1 and Nuclear Red™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.

Platform

Fluorescence microscope

Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	FITC filter set

Example protocol

PREPARATION OF WORKING SOLUTION

DiTO™-1 working solution

Make DiTO™-1 working solution in Hanks with 20 mM Hepes buffer (HH buffer) or buffer of your choice at your desired concentration.
Note In initial experiments, it may be best to try several dye concentrations to determine the optimal concentration that yields the desired result. High dye concentration tends to cause nonspecific staining of other cellular structures.

SAMPLE EXPERIMENTAL PROTOCOL

Caution: The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types and factors may influence staining. Residual detergent on glassware may also affect staining of many organisms, and cause brightly stained material to appear in solutions with or without cells present.

Grow and treat cells as desired.
Remove the cell culture medium and fix cells.
Add DiTO™-1 working solution (1 to 10 µM) into the cells (either suspension or adherent cells), and stain the cells for 15 to 60 minutes.
Remove the dye working solution and add HH buffer or buffer of your choice.
Analyze the cellular staining with a fluorescence microscope using FITC filter.

Calculators

Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	76.759 µL	383.795 µL	767.589 µL	3.838 mL	7.676 mL
5 mM	15.352 µL	76.759 µL	153.518 µL	767.589 µL	1.535 mL
10 mM	7.676 µL	38.379 µL	76.759 µL	383.795 µL	767.589 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	117000¹
Excitation (nm)	514
Emission (nm)	531
Quantum yield	0.34¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
DiTO™-3 [equivalent to TOTO®-3] 5 mM DMSO Solution	642	661	154000¹
Oxazine 1 CAS 24796-94-9	648	668	123000
DiYO™-1 [equivalent to YOYO®-1] 5 mM DMSO Solution	491	508	98900¹
JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] CAS#: 3520-43-2	515	530	195000¹
BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole]	349	421	-
DiYO™-1 [equivalent to YOYO®-1]	491	508	98900¹
DiPO™-1 [equivalent to POPO®-1] 1 mM DMSO Solution	433	457	92000¹

Images

Figure 1. Chemical structure for DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7*

Citations

View all 4 citations: Citation Explorer

Biofilm formation on the surface of monazite and xenotime during bioleaching
Authors: van Alin, Arya and Corbett, Melissa K and Fathollahzadeh, Homayoun and Tjiam, M Christian and Rickard, William DA and Sun, Xiao and Putnis, Andrew and Eksteen, Jacques and Kaksonen, Anna H and Watkin, Elizabeth
Journal: Microbial Biotechnology (2023)

Highly efficient incorporation of dATP in terminal transferase polymerization forming the ploy (A) n-DITO-1 fluorescent probe sensing terminal transferase and T4 polynucleotide kinase activity
Authors: Zhu, Jing and Chen, Liutao
Journal: Analytica Chimica Acta (2022): 340080

Francisella novicida and F. philomiragia biofilm features conditionning fitness in spring water and in presence of antibiotics
Authors: Siebert, Claire and Villers, Corinne and Pavlou, Georgios and Touquet, Bastien and Yakandawala, Nandadeva and Tardieux, Isabelle and Renesto, Patricia
Journal: Plos one (2020): e0228591

References

View all 27 references: Citation Explorer

Chromatin staining of Drosophila testes
Authors: Bonaccorsi S, Giansanti MG, Cenci G, Gatti M.
Journal: Cold Spring Harb Protoc (2012)

In vivo cancer imaging by poly(ethylene glycol)-b-poly(varepsilon-caprolactone) micelles containing a near-infrared probe
Authors: Cho H, Indig GL, Weichert J, Shin HC, Kwon GS.
Journal: Nanomedicine (2012): 228

The fluorescent dyes TO-PRO-3 and TOTO-3 iodide allow detection of microbial cells in soil samples without interference from background fluorescence
Authors: Henneberger R, Birch D, Bergquist P, Walter M, Anitori RP.
Journal: Biotechniques (2011): 190

Effect of Mg ions on efficiency of gene electrotransfer and on cell electropermeabilization
Authors: Haberl S, Miklavcic D, Pavlin M.
Journal: Bioelectrochemistry (2010): 265

Visualizing nuclei in skin cryosections: viable options to 4'6-diamidino-2-phenylindol for confocal laser microscopy
Authors: Glaser K, Wilke K, Wepf R, Biel SS.
Journal: Skin Res Technol (2008): 324

Highly chlorinated Escherichia coli cannot be stained by propidium iodide
Authors: Phe MH, Dossot M, Guilloteau H, Block JC.
Journal: Can J Microbiol (2007): 664

DNA labeling in living cells
Authors: Martin RM, Leonhardt H, Cardoso MC.
Journal: Cytometry A (2005): 45

Quinolinium, 1,1'-[1,3-propanediylbis[(dimethyliminio)-3,1-propanediyl]]bis[4-[3-(3-methyl-2(3 H)-benzothiazolylidene)-1-propen-1-yl]-,iodide (1:4)
Authors: Shan L., undefined
Journal: In: Molecular Imaging and Contrast Agent Database (MICAD), Bethesda (MD). (2004)

Chlorination effect on the fluorescence of nucleic acid staining dyes
Authors: Phe MH, Dossot M, Block JC.
Journal: Water Res (2004): 3729

Sensitive and reliable JC-1 and TOTO-3 double staining to assess mitochondrial transmembrane potential and plasma membrane integrity: interest for cell death investigations
Authors: Zuliani T, Duval R, Jayat C, Schnebert S, Andre P, Dumas M, Ratinaud MH.
Journal: Cytometry A (2003): 100