DiTO™-3 [equivalent to TOTO®-3] *5 mM DMSO Solution*

DiTO™-3 is chemically equivalent to TOTO®-3 (TOTO® is the trademark of Invitrogen). DiTO™-3 is a carbocyanine dimer with far-red fluorescence similar to Cy® 5 dyes. It is cell-impermeant and easily distinguished from fluorescein and rhodamine as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiTO™-3 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Green™ LCS1 dye and cell-impermeant DiTO®-3 can be used to assess cell viability. DiTO™-3 and Nuclear Green™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.