DiTO™-3 [equivalent to TOTO®-3] *5 mM DMSO Solution*

DiTO™-3

equivalent to TOTO®-3; 5 mM DMSO Solution

DiTO™-3 is chemically equivalent to TOTO®-3 (TOTO® is the trademark of Invitrogen). DiTO™-3 is a carbocyanine dimer with far-red fluorescence similar to Cy® 5 dyes. It is cell-impermeant and easily distinguished from fluorescein and rhodamine as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiTO™-3 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Green™ LCS1 dye and cell-impermeant DiTO®-3 can be used to assess cell viability. DiTO™-3 and Nuclear Green™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17576	0.2 ml	Price

References

View all 27 references: Citation Explorer

Chromatin staining of Drosophila testes

Authors:

Bonaccorsi S, Giansanti MG, Cenci G, Gatti M.

Journal:

Cold Spring Harb Protoc (2012)

In vivo cancer imaging by poly(ethylene glycol)-b-poly(varepsilon-caprolactone) micelles containing a near-infrared probe

Authors:

Cho H, Indig GL, Weichert J, Shin HC, Kwon GS.

Journal:

Nanomedicine (2012): 228

The fluorescent dyes TO-PRO-3 and TOTO-3 iodide allow detection of microbial cells in soil samples without interference from background fluorescence

Authors:

Henneberger R, Birch D, Bergquist P, Walter M, Anitori RP.

Journal:

Biotechniques (2011): 190

Effect of Mg ions on efficiency of gene electrotransfer and on cell electropermeabilization

Authors:

Haberl S, Miklavcic D, Pavlin M.

Journal:

Bioelectrochemistry (2010): 265

Visualizing nuclei in skin cryosections: viable options to 4'6-diamidino-2-phenylindol for confocal laser microscopy

Authors:

Glaser K, Wilke K, Wepf R, Biel SS.

Journal:

Skin Res Technol (2008): 324

Physical properties

Molecular weight	1354.85
Solvent	Water

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	154000 ¹
Excitation (nm)	642
Emission (nm)	661
Quantum yield	0.06 ¹

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Instrument settings

Fluorescence microscope
Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Cy5 filter set

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Page updated on October 13, 2025