DiYO™-3 [equivalent to YOYO®-3] 5 mM DMSO Solution CAS 156312-20-8

DiYO™-3 is chemically equivalent to YOYO®-3 (YOYO® is the trademark of Invitrogen). DiYO™-3 is a carbocyanine dimer with far-red fluorescence similar to Cy® 5 dyes. It is cell-impermeant and easily distinguished from fluorescein and rhodamine as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiYO™-3 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Green™ LCS1 dye and cell-impermeant DiYO®-3 can be used to assess cell viability. DiYO™-3 and Nuclear Green™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.

Example protocol

PREPARATION OF WORKING SOLUTION

DiYO™-3 working solution

Make DiYO™-3 working solution in Hanks with 20 mM Hepes buffer (HH buffer) or buffer of your choice at your desired concentration.
Note In initial experiments, it may be best to try several dye concentrations to determine the optimal concentration that yields the desired result. High dye concentration tends to cause nonspecific staining of other cellular structures.

SAMPLE EXPERIMENTAL PROTOCOL

Caution: The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types and factors may influence staining. Residual detergent on glassware may also affect staining of many organisms, and cause brightly stained material to appear in solutions with or without cells present.

Grow and treat cells as desired.
Remove the cell culture medium and fix cells.
Add DiYO™-3 working solution (1 to 10 µM) into the cells (either suspension or adherent cells), and stain the cells for 15 to 60 minutes.
Remove the dye working solution and add HH buffer or buffer of your choice.
Analyze the cellular staining with a fluorescence microscope using Cy5 filter.

Spectrum

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Product family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield
DiYO™-1 [equivalent to YOYO®-1]	491	508	98900¹	0.52¹
DiYO™-1 [equivalent to YOYO®-1] 5 mM DMSO Solution	491	508	98900¹	0.52¹
DiTO™-3 [equivalent to TOTO®-3] 5 mM DMSO Solution	642	661	154000¹	0.06¹

Citations

View all 2 citations: Citation Explorer

Gongying-Jiedu-Xiji recipe promotes the healing of venous ulcers by inhibiting ferroptosis via the CoQ-FSP1 axis
Authors: Lu, Yongpan and Zhao, Dejie and Liu, Ming and Cao, Guoqi and Liu, Chunyan and Yin, Siyuan and Song, Ru and Ma, Jiaxu and Sun, Rui and Wu, Zhenjie and others,
Journal: Frontiers in Pharmacology (2023)

GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca2+ nanoparticles underlies distinct gene therapy effect in ovarian cancer
Authors: Su, Linjia and Sun, Zhe and Qi, Fangzheng and Su, Huishan and Qian, Luomeng and Li, Jing and Zuo, Liang and Huang, Jinhai and Yu, Zhilin and Li, Jinping and others,
Journal: Journal of Nanobiotechnology (2022): 1--20

References

View all 22 references: Citation Explorer

Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524

Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630

Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713

Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29

Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87

Page updated on July 21, 2025

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Catalog Number	17581
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Physical properties

Molecular weight	1322.73
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	167000¹
Excitation (nm)	612
Emission (nm)	631
Quantum yield	0.15¹

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134