DiYO™-3 [equivalent to YOYO®-3] *5 mM DMSO Solution* *CAS 156312-20-8*
![Chemical structure for DiYO™-3 [equivalent to YO-YO®-3] *5 mM DMSO Solution* *CAS 156312-20-8*](https://images.aatbio.com/products/figures-and-data/diyo-3-yo-yo-3-5-mm-dmso-solution-cas-156312-20-8/chemical-structure-of-diyo-3-yo-yo-3-5-mm-dmso-solution-cas-156312-20-8.svg)
| Overview |  SDSProtocol |
See also: Nucleus, Bioconjugation, Cell Cytotoxicity, Cellular Processes, Physiological Probes, DNA and RNA Quantitation, Cell Structures and Organelles
Molecular weight 1322.73 | Extinction coefficient (cm -1 M -1) 1670001 | Excitation (nm) 612 | Emission (nm) 631 | Quantum yield 0.151 |
DiYO™-3 is chemically equivalent to YOYO®-3 (YOYO® is the trademark of Invitrogen). DiYO™-3 is a carbocyanine dimer with far-red fluorescence similar to Cy® 5 dyes. It is cell-impermeant and easily distinguished from fluorescein and rhodamine as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiYO™-3 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Green™ LCS1 dye and cell-impermeant DiYO®-3 can be used to assess cell viability. DiYO™-3 and Nuclear Green™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.
Platform
Fluorescence microscope
| Excitation | Cy5 filter set |
| Emission | Cy5 filter set |
| Recommended plate | Black wall/clear bottom |
| Instrument specification(s) | Cy5 filter set |
Example protocol
PREPARATION OF WORKING SOLUTION
DiYO™-3 working solution
Make DiYO™-3 working solution in Hanks with 20 mM Hepes buffer (HH buffer) or buffer of your choice at your desired concentration.Note In initial experiments, it may be best to try several dye concentrations to determine the optimal concentration that yields the desired result. High dye concentration tends to cause nonspecific staining of other cellular structures.
SAMPLE EXPERIMENTAL PROTOCOL
Caution: The following protocol can be adapted for most cell types. Growth medium, cell density, the presence of other cell types and factors may influence staining. Residual detergent on glassware may also affect staining of many organisms, and cause brightly stained material to appear in solutions with or without cells present.
- Grow and treat cells as desired.
- Remove the cell culture medium and fix cells.
- Add DiYO™-3 working solution (1 to 10 µM) into the cells (either suspension or adherent cells), and stain the cells for 15 to 60 minutes.
- Remove the dye working solution and add HH buffer or buffer of your choice.
- Analyze the cellular staining with a fluorescence microscope using Cy5 filter.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiYO™-3 [equivalent to YOYO®-3] *5 mM DMSO Solution* *CAS 156312-20-8* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 75.601 µL | 378.006 µL | 756.012 µL | 3.78 mL | 7.56 mL |
| 5 mM | 15.12 µL | 75.601 µL | 151.202 µL | 756.012 µL | 1.512 mL |
| 10 mM | 7.56 µL | 37.801 µL | 75.601 µL | 378.006 µL | 756.012 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
| / | = | x | = |
Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 1670001 |
| Excitation (nm) | 612 |
| Emission (nm) | 631 |
| Quantum yield | 0.151 |
Images
Figure 1. Chemical structure for DiYO™-3 [equivalent to YO-YO®-3] *5 mM DMSO Solution* *CAS 156312-20-8*
References
View all 22 references: Citation Explorer
Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524
Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
DNA length evaluation using cyanine dye and fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Tsuruoka M.
Journal: Biomacromolecules (2005): 2703
Authors: Shimizu M, Sasaki S, Tsuruoka M.
Journal: Biomacromolecules (2005): 2703
Implementation of accurate and fast DNA cytometry by confocal microscopy in 3D
Authors: Ploeger LS, Huisman A, van der Gugten J, van der Giezen DM, Belien JA, Abbaker AY, Dullens HF, Grizzle W, Poulin NM, Meijer GA, van Diest PJ.
Journal: Cell Oncol (2005): 225
Authors: Ploeger LS, Huisman A, van der Gugten J, van der Giezen DM, Belien JA, Abbaker AY, Dullens HF, Grizzle W, Poulin NM, Meijer GA, van Diest PJ.
Journal: Cell Oncol (2005): 225
TO-PRO-3 is an optimal fluorescent dye for nuclear counterstaining in dual-colour FISH on paraffin sections
Authors: Bink K, Walch A, Feuchtinger A, Eisenmann H, Hutzler P, Hofler H, Werner M.
Journal: Histochem Cell Biol (2001): 293
Authors: Bink K, Walch A, Feuchtinger A, Eisenmann H, Hutzler P, Hofler H, Werner M.
Journal: Histochem Cell Biol (2001): 293
Oxazole yellow homodimer YOYO-1-labeled DNA: a fluorescent complex that can be used to assess structural changes in DNA following formation and cellular delivery of cationic lipid DNA complexes
Authors: Wong M, Kong S, Dragowska WH, Bally MB.
Journal: Biochim Biophys Acta (2001): 61
Authors: Wong M, Kong S, Dragowska WH, Bally MB.
Journal: Biochim Biophys Acta (2001): 61
Photophysical properties of fluorescent DNA-dyes bound to single- and double-stranded DNA in aqueous buffered solution
Authors: Cosa G, Focsaneanu KS, McLean JR, McNamee JP, Scaiano JC.
Journal: Photochem Photobiol (2001): 585
Authors: Cosa G, Focsaneanu KS, McLean JR, McNamee JP, Scaiano JC.
Journal: Photochem Photobiol (2001): 585
Application notes
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