DiYO™-3 [equivalent to YOYO®-3] *5 mM DMSO Solution* *CAS 156312-20-8*

DiYO™-3

equivalent to YOYO®-3; 5 mM DMSO Solution; CAS 156312-20-8

DiYO™-3 is chemically equivalent to YOYO®-3 (YOYO® is the trademark of Invitrogen). DiYO™-3 is a carbocyanine dimer with far-red fluorescence similar to Cy® 5 dyes. It is cell-impermeant and easily distinguished from fluorescein and rhodamine as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiYO™-3 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Green™ LCS1 dye and cell-impermeant DiYO®-3 can be used to assess cell viability. DiYO™-3 and Nuclear Green™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.

Protocol

SDS

Catalog	Size	Price	Quantity
17581	0.2 ml	Price

Citations

View all 2 citations: Citation Explorer

Gongying-Jiedu-Xiji recipe promotes the healing of venous ulcers by inhibiting ferroptosis via the CoQ-FSP1 axis

Authors:

Lu, Yongpan and Zhao, Dejie and Liu, Ming and Cao, Guoqi and Liu, Chunyan and Yin, Siyuan and Song, Ru and Ma, Jiaxu and Sun, Rui and Wu, Zhenjie and others,

Journal:

Frontiers in Pharmacology (2023)

GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca2+ nanoparticles underlies distinct gene therapy effect in ovarian cancer

Authors:

Su, Linjia and Sun, Zhe and Qi, Fangzheng and Su, Huishan and Qian, Luomeng and Li, Jing and Zuo, Liang and Huang, Jinhai and Yu, Zhilin and Li, Jinping and others,

Journal:

Journal of Nanobiotechnology (2022): 1--20

References

View all 22 references: Citation Explorer

Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice

Authors:

Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.

Journal:

Stroke (2012): 524

Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence

Authors:

Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.

Journal:

Lasers Surg Med (2010): 630

Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques

Authors:

van 't Hoff M, Reuter M, Dryden DT, Oheim M.

Journal:

Phys Chem Chem Phys (2009): 7713

Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians

Authors:

Lee BC, Kim S, Soh KS.

Journal:

J Acupunct Meridian Stud (2008): 29

Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy

Authors:

Shimizu M, Sasaki S, Kinjo M.

Journal:

Anal Biochem (2007): 87

Physical properties

Molecular weight	1322.73
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	167000 ¹
Excitation (nm)	612
Emission (nm)	631
Quantum yield	0.15 ¹

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Instrument settings

Fluorescence microscope
Excitation	Cy5 filter set
Emission	Cy5 filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	Cy5 filter set

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Cell Viability & Proliferation
Brochure: Nucleic Acid Detection Probes & Assay Kits

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Page updated on October 29, 2025