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DiYO™-3
equivalent to YOYO®-3; 5 mM DMSO Solution; CAS 156312-20-8
DiYO™-3 is chemically equivalent to YOYO®-3 (YOYO® is the trademark of Invitrogen). DiYO™-3 is a carbocyanine dimer with far-red fluorescence similar to Cy® 5 dyes. It is cell-impermeant and easily distinguished from fluorescein and rhodamine as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiYO™-3 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Green™ LCS1 dye and cell-impermeant DiYO®-3 can be used to assess cell viability. DiYO™-3 and Nuclear Green™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.
Chemical structure for DiYO™-3  [equivalent to YO-YO®-3] *5 mM DMSO Solution* *CAS 156312-20-8*
Chemical structure for DiYO™-3  [equivalent to YO-YO®-3] *5 mM DMSO Solution* *CAS 156312-20-8*
CatalogSize
Price
Quantity
175810.2 ml
Price
 
Physical properties

Molecular weight1322.73
SolventDMSO
Spectral properties

Extinction coefficient (cm -1 M -1)
167000
1
Excitation (nm)612
Emission (nm)631
Quantum yield
0.15
1
Storage, safety and handling

H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134
Instrument settings

Fluorescence microscope
ExcitationCy5 filter set
EmissionCy5 filter set
Recommended plateBlack wall/clear bottom
Instrument specification(s)Cy5 filter set
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Page updated on October 3, 2025