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DiYO™-1 [equivalent to YOYO®-1]

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Physical properties
Molecular weight1270.65
Spectral properties
Extinction coefficient (cm -1 M -1)989001
Excitation (nm)491
Emission (nm)508
Quantum yield0.521
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
DiYO™-1 is chemically equivalent to YOYO®-1 (YOYO® is the trademark of Invitrogen). DiYO™-1 is a carbocyanine dimer with green fluorescence similar to FITC. It is cell-impermeant and easily distinguished from Cy5 and rhodamines as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiYO™-1 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Red™ LCS1 dye and cell-impermeant DiYO®-1 can be used to assess cell viability. DiYO™-1 and Nuclear Red™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiYO™-1 [equivalent to YOYO®-1] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM78.7 µL393.499 µL786.999 µL3.935 mL7.87 mL
5 mM15.74 µL78.7 µL157.4 µL786.999 µL1.574 mL
10 mM7.87 µL39.35 µL78.7 µL393.499 µL786.999 µL

Molarity calculator

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Spectral properties

Extinction coefficient (cm -1 M -1)989001
Excitation (nm)491
Emission (nm)508
Quantum yield0.521



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Correction: Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging
Authors: Pyle, J. R., Chen, J.
Journal: Beilstein J Nanotechnol (2018): 809-811
Ultrafast Transient Absorption Spectra of Photoexcited YOYO-1 molecules call for additional investigations of their fluorescence quenching mechanism
Authors: Wang, L., Pyle, J. R., Cimatu, K. A., Chen, J.
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Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging
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Detection of amyloid-beta fibrils using the DNA-intercalating dye YOYO-1: Binding mode and fibril formation kinetics
Authors: Lindberg, D. J., Esbjorner, E. K.
Journal: Biochem Biophys Res Commun (2016): 313-8
Interference of ATP with the fluorescent probes YOYO-1 andYOYO-3 modifies the mechanical properties of intercalator-stained DNA confined in nanochannels
Authors: Roushan, M., Azad, Z., Lim, S. F., Wang, H., Riehn, R.
Journal: Mikrochim Acta (2015): 1561-1565


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