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DiYO™-1 [equivalent to YOYO®-1]
DiYO™-1 is chemically equivalent to YOYO®-1 (YOYO® is the trademark of Invitrogen). DiYO™-1 is a carbocyanine dimer with green fluorescence similar to FITC. It is cell-impermeant and easily distinguished from Cy5 and rhodamines as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiYO™-1 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Red™ LCS1 dye and cell-impermeant DiYO®-1 can be used to assess cell viability. DiYO™-1 and Nuclear Red™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DiYO™-1 [equivalent to YOYO®-1] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 78.7 µL | 393.499 µL | 786.999 µL | 3.935 mL | 7.87 mL |
| 5 mM | 15.74 µL | 78.7 µL | 157.4 µL | 786.999 µL | 1.574 mL |
| 10 mM | 7.87 µL | 39.35 µL | 78.7 µL | 393.499 µL | 786.999 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Product family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
| DiYO™-3 [equivalent to YOYO®-3] *5 mM DMSO Solution* *CAS 156312-20-8* | 612 | 631 | 1670001 | 0.151 |
| DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution* | 433 | 457 | 920001 | 0.61 |
| DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7* | 514 | 531 | 1170001 | 0.341 |
Citations
View all 28 citations: Citation Explorer
Single-Virus Microscopy of Biochemical Events in Viral Entry
Authors: Cervantes, Marcos and Mannsverk, Steinar and Hess, Tobin and Filipe, Diogo and Villamil Giraldo, Ana and Kasson, Peter M
Journal: JACS Au (2024)
Authors: Cervantes, Marcos and Mannsverk, Steinar and Hess, Tobin and Filipe, Diogo and Villamil Giraldo, Ana and Kasson, Peter M
Journal: JACS Au (2024)
Trellis tree-based analysis reveals stromal regulation of patient-derived organoid drug responses
Authors: Zapatero, Mar{\'\i}a Ramos and Tong, Alexander and Opzoomer, James W and O’Sullivan, Rhianna and Rodriguez, Ferran Cardoso and Sufi, Jahangir and Vlckova, Petra and Nattress, Callum and Qin, Xiao and Claus, Jeroen and others,
Journal: Cell (2023): 5606--5619
Authors: Zapatero, Mar{\'\i}a Ramos and Tong, Alexander and Opzoomer, James W and O’Sullivan, Rhianna and Rodriguez, Ferran Cardoso and Sufi, Jahangir and Vlckova, Petra and Nattress, Callum and Qin, Xiao and Claus, Jeroen and others,
Journal: Cell (2023): 5606--5619
Extracellular DNA: A Critical Aspect of Marine Biofilms
Authors: Tuck, Benjamin and Salgar-Chaparro, Silvia J and Watkin, Elizabeth and Somers, Anthony and Forsyth, Maria and Machuca, Laura L
Journal: Microorganisms (2022): 1285
Authors: Tuck, Benjamin and Salgar-Chaparro, Silvia J and Watkin, Elizabeth and Somers, Anthony and Forsyth, Maria and Machuca, Laura L
Journal: Microorganisms (2022): 1285
A viral ubiquitination switch attenuates innate immunity and triggers nuclear import of virion DNA and infection
Authors: Bauer, Michael and Gomez-Gonzalez, Alfonso and Suomalainen, Maarit and Schilling, Nicolas and Hemmi, Silvio and Greber, Urs F
Journal: Science advances (2021): eabl7150
Authors: Bauer, Michael and Gomez-Gonzalez, Alfonso and Suomalainen, Maarit and Schilling, Nicolas and Hemmi, Silvio and Greber, Urs F
Journal: Science advances (2021): eabl7150
Non-viral gene delivery of the oncotoxic protein NS1 for treatment of hepatocellular carcinoma
Authors: Witzigmann, Dominik and Grossen, Philip and Quintavalle, Cristina and Lanzafame, Manuela and Schenk, Susanne H and Tran, Xue-Ting and Englinger, Bernhard and Hauswirth, Patrick and Gr{\"u}nig, David and van Schoonhoven, Sushilla and others,
Journal: Journal of Controlled Release (2021): 138--152
Authors: Witzigmann, Dominik and Grossen, Philip and Quintavalle, Cristina and Lanzafame, Manuela and Schenk, Susanne H and Tran, Xue-Ting and Englinger, Bernhard and Hauswirth, Patrick and Gr{\"u}nig, David and van Schoonhoven, Sushilla and others,
Journal: Journal of Controlled Release (2021): 138--152
References
View all 22 references: Citation Explorer
Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524
Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
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