DiYO™-1 [equivalent to YOYO®-1]

DiYO™-1

equivalent to YOYO®-1

DiYO™-1 is chemically equivalent to YOYO®-1 (YOYO® is the trademark of Invitrogen). DiYO™-1 is a carbocyanine dimer with green fluorescence similar to FITC. It is cell-impermeant and easily distinguished from Cy5 and rhodamines as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiYO™-1 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Red™ LCS1 dye and cell-impermeant DiYO®-1 can be used to assess cell viability. DiYO™-1 and Nuclear Red™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
17579	1 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiYO™-1 [equivalent to YOYO®-1] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	78.7 µL	393.499 µL	786.999 µL	3.935 mL	7.87 mL
5 mM	15.74 µL	78.7 µL	157.4 µL	786.999 µL	1.574 mL
10 mM	7.87 µL	39.35 µL	78.7 µL	393.499 µL	786.999 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1270.65 g/mol	=	mL	x	mM	=	mmol

Citations

View all 28 citations: Citation Explorer

Single-Virus Microscopy of Biochemical Events in Viral Entry

Authors:

Cervantes, Marcos and Mannsverk, Steinar and Hess, Tobin and Filipe, Diogo and Villamil Giraldo, Ana and Kasson, Peter M

Journal:

JACS Au (2024)

Trellis tree-based analysis reveals stromal regulation of patient-derived organoid drug responses

Authors:

Zapatero, Mar{\'\i}a Ramos and Tong, Alexander and Opzoomer, James W and O’Sullivan, Rhianna and Rodriguez, Ferran Cardoso and Sufi, Jahangir and Vlckova, Petra and Nattress, Callum and Qin, Xiao and Claus, Jeroen and others,

Journal:

Cell (2023): 5606--5619

Extracellular DNA: A Critical Aspect of Marine Biofilms

Authors:

Tuck, Benjamin and Salgar-Chaparro, Silvia J and Watkin, Elizabeth and Somers, Anthony and Forsyth, Maria and Machuca, Laura L

Journal:

Microorganisms (2022): 1285

A viral ubiquitination switch attenuates innate immunity and triggers nuclear import of virion DNA and infection

Authors:

Bauer, Michael and Gomez-Gonzalez, Alfonso and Suomalainen, Maarit and Schilling, Nicolas and Hemmi, Silvio and Greber, Urs F

Journal:

Science advances (2021): eabl7150

Non-viral gene delivery of the oncotoxic protein NS1 for treatment of hepatocellular carcinoma

Authors:

Witzigmann, Dominik and Grossen, Philip and Quintavalle, Cristina and Lanzafame, Manuela and Schenk, Susanne H and Tran, Xue-Ting and Englinger, Bernhard and Hauswirth, Patrick and Gr{\"u}nig, David and van Schoonhoven, Sushilla and others,

Journal:

Journal of Controlled Release (2021): 138--152

References

View all 22 references: Citation Explorer

Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice

Authors:

Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.

Journal:

Stroke (2012): 524

Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence

Authors:

Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.

Journal:

Lasers Surg Med (2010): 630

Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques

Authors:

van 't Hoff M, Reuter M, Dryden DT, Oheim M.

Journal:

Phys Chem Chem Phys (2009): 7713

Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians

Authors:

Lee BC, Kim S, Soh KS.

Journal:

J Acupunct Meridian Stud (2008): 29

Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy

Authors:

Shimizu M, Sasaki S, Kinjo M.

Journal:

Anal Biochem (2007): 87

Physical properties

Molecular weight	1270.65
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	98900 ¹
Excitation (nm)	491
Emission (nm)	508
Quantum yield	0.52 ¹

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134
CAS	143413-85-8

Documents

Protocol
Safety Data Sheet
Certificate of Analysis

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Page updated on October 24, 2025