DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution*
Ordering information
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Additional ordering information
Telephone | 1-800-990-8053 |
Fax | 1-800-609-2943 |
sales@aatbio.com | |
International | See distributors |
Bulk request | Inquire |
Custom size | Inquire |
Shipping | Standard overnight for United States, inquire for international |
Physical properties
Molecular weight | 1170.54 |
Solvent | Water |
Spectral properties
Extinction coefficient (cm -1 M -1) | 920001 |
Excitation (nm) | 433 |
Emission (nm) | 457 |
Quantum yield | 0.61 |
Storage, safety and handling
H-phrase | H303, H313, H333 |
Hazard symbol | XN |
Intended use | Research Use Only (RUO) |
R-phrase | R20, R21, R22 |
Storage | Freeze (< -15 °C); Minimize light exposure |
UNSPSC | 12171501 |
Overview | SDSProtocol |
See also: Nucleus
CAS 169454-15-3 | Molecular weight 1170.54 | Extinction coefficient (cm -1 M -1) 920001 | Excitation (nm) 433 | Emission (nm) 457 | Quantum yield 0.61 |
DiPO™-1 is the same compound to POPO®-1 (POPO® is the trademark of ThermoFisher). DiPO™-1 is a high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer that is essentially nonfluorescent in the absence of nucleic acids that exhibits excitation/emission maxima ~434/456 nm when bound to nucleic acids. POPO-1 is a Blue emitting dye that can be excited by the 405 laser and emission captured by the 450/50 filter. It is commonly used as a nucleic acid stain in flow cytometry and fluorescence microscopy.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 85.431 µL | 427.153 µL | 854.307 µL | 4.272 mL | 8.543 mL |
5 mM | 17.086 µL | 85.431 µL | 170.861 µL | 854.307 µL | 1.709 mL |
10 mM | 8.543 µL | 42.715 µL | 85.431 µL | 427.153 µL | 854.307 µL |
Molarity calculator
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Spectrum
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Spectral properties
Extinction coefficient (cm -1 M -1) | 920001 |
Excitation (nm) | 433 |
Emission (nm) | 457 |
Quantum yield | 0.61 |
Product Family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
Oxazine 1 *CAS 24796-94-9* | 648 | 668 | 123000 |
DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7* | 514 | 531 | 1170001 |
DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution* | 491 | 508 | 989001 |
JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2* | 515 | 530 | 1950001 |
BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole] | 349 | 421 | - |
DiYO™-1 [equivalent to YOYO®-1] | 491 | 508 | 989001 |
EthD-1 [Ethidium Homodimer-1] | 528 | 617 | - |
Images
References
View all 6 references: Citation Explorer
Determination of restriction enzyme activity when cutting DNA labeled with the TOTO dye family.
Authors: Maschmann, April and Kounovsky-Shafer, Kristy L
Journal: Nucleosides, nucleotides & nucleic acids (2017): 406-417
Authors: Maschmann, April and Kounovsky-Shafer, Kristy L
Journal: Nucleosides, nucleotides & nucleic acids (2017): 406-417
Myosin Va and kinesin II motor proteins are concentrated in ribosomal domains (periaxoplasmic ribosomal plaques) of myelinated axons.
Authors: Sotelo-Silveira, José R and Calliari, Aldo and Cárdenas, Magdalena and Koenig, Edward and Sotelo, José R
Journal: Journal of neurobiology (2004): 187-96
Authors: Sotelo-Silveira, José R and Calliari, Aldo and Cárdenas, Magdalena and Koenig, Edward and Sotelo, José R
Journal: Journal of neurobiology (2004): 187-96
Analysis of dimeric cyanine-nucleic acid dyes by capillary zone electrophoresis in N,N-dimethylacetamide as non-aqueous organic solvent.
Authors: Muzikar, Jan and Rozing, Gerard and van de Goor, Tom and Eberwein, Christine and Kenndler, Ernst
Journal: Journal of chromatography. A (2002): 249-55
Authors: Muzikar, Jan and Rozing, Gerard and van de Goor, Tom and Eberwein, Christine and Kenndler, Ernst
Journal: Journal of chromatography. A (2002): 249-55
Efficient detection of single DNA fragments in flowing sample streams by two-photon fluorescence excitation.
Authors: Van Orden, A and Cai, H and Goodwin, P M and Keller, R A
Journal: Analytical chemistry (1999): 2108-16
Authors: Van Orden, A and Cai, H and Goodwin, P M and Keller, R A
Journal: Analytical chemistry (1999): 2108-16
DNA-binding and fluorescence properties of the DNA bis-intercalating purple oxazole dimer POPO-1.
Authors: Winter, S and Loeber, G
Journal: Journal of biomedical optics (1997): 125-30
Authors: Winter, S and Loeber, G
Journal: Journal of biomedical optics (1997): 125-30
Fluorescently Labeled Virus Probes Show that Natural Virus Populations Can Control the Structure of Marine Microbial Communities.
Authors: Hennes, K P and Suttle, C A and Chan, A M
Journal: Applied and environmental microbiology (1995): 3623-7
Authors: Hennes, K P and Suttle, C A and Chan, A M
Journal: Applied and environmental microbiology (1995): 3623-7
Application notes
A Novel Fluorescent Probe for Imaging and Detecting Hydroxyl Radical in Living Cells
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
Fluorescent Oligonucleotide Labeling Reagents
Monitoring of Mitochondrial Membrane Potential Changes in Live Cells Using JC-10
Selective Analysis of RNA in Live and Fixed Cells with StrandBrite RNA Green
Cell Loading Protocol For Fluorescent pH Indicator, BCECF-AM
FAQ
I ordered your phalloidin-amine (Cat #5302) so I can conjugate it to my oligo. Do you have a recommended protocol I can use?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?
What dye works best for staining and tracking lysosomes in live cells for several hours?
How can I lyse my cells without lysing the nuclear membrane?
Do you have any dual-fluorescence nucleic acid stains that interact with both DNA and RNA?
Do you have any fixable mitochondria staining assay kits?