DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution*
Ordering information
| Price | |
| Catalog Number | |
| Unit Size | |
| Quantity |
Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| Quotation | Request |
| International | See distributors |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 1170.54 |
| Solvent | Water |
Spectral properties
| Extinction coefficient (cm -1 M -1) | 920001 |
| Excitation (nm) | 433 |
| Emission (nm) | 457 |
| Quantum yield | 0.61 |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
See also: Cell Structures and Organelles, Nucleus
CAS 169454-15-3 | Molecular weight 1170.54 | Extinction coefficient (cm -1 M -1) 920001 | Excitation (nm) 433 | Emission (nm) 457 | Quantum yield 0.61 |
DiPO™-1 is the same compound to POPO®-1 (POPO® is the trademark of ThermoFisher). DiPO™-1 is a high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer that is essentially nonfluorescent in the absence of nucleic acids that exhibits excitation/emission maxima ~434/456 nm when bound to nucleic acids. POPO-1 is a Blue emitting dye that can be excited by the 405 laser and emission captured by the 450/50 filter. It is commonly used as a nucleic acid stain in flow cytometry and fluorescence microscopy.
Calculators
Common stock solution preparation
Table 1. Volume of Water needed to reconstitute specific mass of DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 85.431 µL | 427.153 µL | 854.307 µL | 4.272 mL | 8.543 mL |
| 5 mM | 17.086 µL | 85.431 µL | 170.861 µL | 854.307 µL | 1.709 mL |
| 10 mM | 8.543 µL | 42.715 µL | 85.431 µL | 427.153 µL | 854.307 µL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Spectrum
Open in Advanced Spectrum Viewer
Spectral properties
| Extinction coefficient (cm -1 M -1) | 920001 |
| Excitation (nm) | 433 |
| Emission (nm) | 457 |
| Quantum yield | 0.61 |
Product Family
| Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) |
| Oxazine 1 *CAS 24796-94-9* | 648 | 668 | 123000 |
| DiTO™-1 [equivalent to TOTO®-1] *5 mM DMSO Solution* *CAS 143413-84-7* | 514 | 531 | 1170001 |
| DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution* | 491 | 508 | 989001 |
| JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] *CAS#: 3520-43-2* | 515 | 530 | 1950001 |
| BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole] | 349 | 421 | - |
| DiYO™-1 [equivalent to YOYO®-1] | 491 | 508 | 989001 |
Images
Figure 1. Chemical structure for DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution*.
References
View all 6 references: Citation Explorer
Determination of restriction enzyme activity when cutting DNA labeled with the TOTO dye family.
Authors: Maschmann, April and Kounovsky-Shafer, Kristy L
Journal: Nucleosides, nucleotides & nucleic acids (2017): 406-417
Authors: Maschmann, April and Kounovsky-Shafer, Kristy L
Journal: Nucleosides, nucleotides & nucleic acids (2017): 406-417
Myosin Va and kinesin II motor proteins are concentrated in ribosomal domains (periaxoplasmic ribosomal plaques) of myelinated axons.
Authors: Sotelo-Silveira, José R and Calliari, Aldo and Cárdenas, Magdalena and Koenig, Edward and Sotelo, José R
Journal: Journal of neurobiology (2004): 187-96
Authors: Sotelo-Silveira, José R and Calliari, Aldo and Cárdenas, Magdalena and Koenig, Edward and Sotelo, José R
Journal: Journal of neurobiology (2004): 187-96
Analysis of dimeric cyanine-nucleic acid dyes by capillary zone electrophoresis in N,N-dimethylacetamide as non-aqueous organic solvent.
Authors: Muzikar, Jan and Rozing, Gerard and van de Goor, Tom and Eberwein, Christine and Kenndler, Ernst
Journal: Journal of chromatography. A (2002): 249-55
Authors: Muzikar, Jan and Rozing, Gerard and van de Goor, Tom and Eberwein, Christine and Kenndler, Ernst
Journal: Journal of chromatography. A (2002): 249-55
Efficient detection of single DNA fragments in flowing sample streams by two-photon fluorescence excitation.
Authors: Van Orden, A and Cai, H and Goodwin, P M and Keller, R A
Journal: Analytical chemistry (1999): 2108-16
Authors: Van Orden, A and Cai, H and Goodwin, P M and Keller, R A
Journal: Analytical chemistry (1999): 2108-16
DNA-binding and fluorescence properties of the DNA bis-intercalating purple oxazole dimer POPO-1.
Authors: Winter, S and Loeber, G
Journal: Journal of biomedical optics (1997): 125-30
Authors: Winter, S and Loeber, G
Journal: Journal of biomedical optics (1997): 125-30
Fluorescently Labeled Virus Probes Show that Natural Virus Populations Can Control the Structure of Marine Microbial Communities.
Authors: Hennes, K P and Suttle, C A and Chan, A M
Journal: Applied and environmental microbiology (1995): 3623-7
Authors: Hennes, K P and Suttle, C A and Chan, A M
Journal: Applied and environmental microbiology (1995): 3623-7