AAT Bioquest

DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution*

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Physical properties
Molecular weight1170.54
Spectral properties
Extinction coefficient (cm -1 M -1)920001
Excitation (nm)433
Emission (nm)457
Quantum yield0.61
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


See also: Nucleus
Molecular weight
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
Quantum yield
DiPO™-1 is the same compound to POPO®-1 (POPO® is the trademark of ThermoFisher). DiPO™-1 is a high-affinity nucleic acid stain based upon a symmetric cyanine dye dimer that is essentially nonfluorescent in the absence of nucleic acids that exhibits excitation/emission maxima ~434/456 nm when bound to nucleic acids. POPO-1 is a Blue emitting dye that can be excited by the 405 laser and emission captured by the 450/50 filter. It is commonly used as a nucleic acid stain in flow cytometry and fluorescence microscopy.


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of DiPO™-1 [equivalent to POPO®-1] *1 mM DMSO Solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM85.431 µL427.153 µL854.307 µL4.272 mL8.543 mL
5 mM17.086 µL85.431 µL170.861 µL854.307 µL1.709 mL
10 mM8.543 µL42.715 µL85.431 µL427.153 µL854.307 µL

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Spectral properties

Extinction coefficient (cm -1 M -1)920001
Excitation (nm)433
Emission (nm)457
Quantum yield0.61



View all 6 references: Citation Explorer
Determination of restriction enzyme activity when cutting DNA labeled with the TOTO dye family.
Authors: Maschmann, April and Kounovsky-Shafer, Kristy L
Journal: Nucleosides, nucleotides & nucleic acids (2017): 406-417
Myosin Va and kinesin II motor proteins are concentrated in ribosomal domains (periaxoplasmic ribosomal plaques) of myelinated axons.
Authors: Sotelo-Silveira, José R and Calliari, Aldo and Cárdenas, Magdalena and Koenig, Edward and Sotelo, José R
Journal: Journal of neurobiology (2004): 187-96
Analysis of dimeric cyanine-nucleic acid dyes by capillary zone electrophoresis in N,N-dimethylacetamide as non-aqueous organic solvent.
Authors: Muzikar, Jan and Rozing, Gerard and van de Goor, Tom and Eberwein, Christine and Kenndler, Ernst
Journal: Journal of chromatography. A (2002): 249-55
Efficient detection of single DNA fragments in flowing sample streams by two-photon fluorescence excitation.
Authors: Van Orden, A and Cai, H and Goodwin, P M and Keller, R A
Journal: Analytical chemistry (1999): 2108-16
DNA-binding and fluorescence properties of the DNA bis-intercalating purple oxazole dimer POPO-1.
Authors: Winter, S and Loeber, G
Journal: Journal of biomedical optics (1997): 125-30
Fluorescently Labeled Virus Probes Show that Natural Virus Populations Can Control the Structure of Marine Microbial Communities.
Authors: Hennes, K P and Suttle, C A and Chan, A M
Journal: Applied and environmental microbiology (1995): 3623-7