DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution*

DiYO™-1

equivalent to YOYO®-1; 5 mM DMSO Solution

DiYO™-1 is chemically equivalent to YOYO®-1 (YOYO® is the trademark of Invitrogen). DiYO™-1 is a carbocyanine dimer with green fluorescence similar to FITC. It is cell-impermeant and easily distinguished from Cy5 and rhodamines as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiYO™-1 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Red™ LCS1 dye and cell-impermeant DiYO®-1 can be used to assess cell viability. DiYO™-1 and Nuclear Red™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.

Protocol

SDS

Catalog	Size	Price	Quantity
17580	0.2 ml	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	78.7 µL	393.499 µL	786.999 µL	3.935 mL	7.87 mL
5 mM	15.74 µL	78.7 µL	157.4 µL	786.999 µL	1.574 mL
10 mM	7.87 µL	39.35 µL	78.7 µL	393.499 µL	786.999 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	1270.65 g/mol	=	mL	x	mM	=	mmol

Citations

View all 11 citations: Citation Explorer

A complete model of mouse embryogenesis through organogenesis enabled by chemically induced embryo founder cells

Authors:

Li, Huanhuan and Guan, Wei and Huang, Jiahui and Shen, Penglei and Wu, Jinyi and Luo, Haiping and Yang, Yun and Ning, Shaoqiang and Chang, Litao and Zhao, Haiyong and others,

Journal:

Cell (2025)

Single-Virus Microscopy of Biochemical Events in Viral Entry

Authors:

Cervantes, Marcos and Mannsverk, Steinar and Hess, Tobin and Filipe, Diogo and Villamil Giraldo, Ana and Kasson, Peter M

Journal:

JACS Au (2024)

Self-modifying NanoEnhancers facilitating lysosomal escape for cGAS-STING cascading activation in tumor immunotherapy

Authors:

Ge, Yanni and Bao, Yuheng and Shao, An and Jin, Kai and Mao, Zhengwei and Tong, Weijun and Ye, Juan

Journal:

Nano Today (2024): 102391

Protective Effect of Ergothioneine against Oxidative Stress-Induced Chondrocyte Death

Authors:

Sakata, Shuzo and Kunimatsu, Ryo and Tanimoto, Kotaro

Journal:

Antioxidants (2024): 800

The RIPK1 death domain restrains ZBP1-and TRIF-mediated cell death and inflammation

Authors:

Imai, Takashi and Lin, Juan and Kaya, G{\"o}ksu G{\"o}kberk and Ju, Eunjin and Kondylis, Vangelis and Kelepouras, Konstantinos and Liccardi, Gianmaria and Kim, Chun and Pasparakis, Manolis

Journal:

Immunity (2024)

References

View all 22 references: Citation Explorer

Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice

Authors:

Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.

Journal:

Stroke (2012): 524

Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence

Authors:

Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.

Journal:

Lasers Surg Med (2010): 630

Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques

Authors:

van 't Hoff M, Reuter M, Dryden DT, Oheim M.

Journal:

Phys Chem Chem Phys (2009): 7713

Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians

Authors:

Lee BC, Kim S, Soh KS.

Journal:

J Acupunct Meridian Stud (2008): 29

Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy

Authors:

Shimizu M, Sasaki S, Kinjo M.

Journal:

Anal Biochem (2007): 87

Physical properties

Molecular weight	1270.65
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	98900 ¹
Excitation (nm)	491
Emission (nm)	508
Quantum yield	0.52 ¹

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134
CAS	143413-85-8

Instrument settings

Fluorescence microscope
Excitation	FITC filter set
Emission	FITC filter set
Recommended plate	Black wall/clear bottom
Instrument specification(s)	FITC filter set

Documents

Protocol
Safety Data Sheet
Certificate of Analysis
Brochure: Cell Viability & Proliferation
Brochure: Nucleic Acid Detection Probes & Assay Kits

Telephone
Fax
Email	sales@aatbio.com
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Purchase order	Send to sales@aatbio.com
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Page updated on October 28, 2025