logo
AAT Bioquest

DiYO™-1 [equivalent to YOYO®-1]

DiYO™-1 is chemically equivalent to YOYO®-1 (YOYO® is the trademark of Invitrogen). DiYO™-1 is a carbocyanine dimer with green fluorescence similar to FITC. It is cell-impermeant and easily distinguished from Cy5 and rhodamines as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiYO™-1 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Red™ LCS1 dye and cell-impermeant DiYO®-1 can be used to assess cell viability. DiYO™-1 and Nuclear Red™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiYO™-1 [equivalent to YOYO®-1] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM78.7 µL393.499 µL786.999 µL3.935 mL7.87 mL
5 mM15.74 µL78.7 µL157.4 µL786.999 µL1.574 mL
10 mM7.87 µL39.35 µL78.7 µL393.499 µL786.999 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
/=x=

Spectrum

Citations

View all 27 citations: Citation Explorer
Trellis tree-based analysis reveals stromal regulation of patient-derived organoid drug responses
Authors: Zapatero, Mar{\'\i}a Ramos and Tong, Alexander and Opzoomer, James W and O’Sullivan, Rhianna and Rodriguez, Ferran Cardoso and Sufi, Jahangir and Vlckova, Petra and Nattress, Callum and Qin, Xiao and Claus, Jeroen and others,
Journal: Cell (2023): 5606--5619
Extracellular DNA: A Critical Aspect of Marine Biofilms
Authors: Tuck, Benjamin and Salgar-Chaparro, Silvia J and Watkin, Elizabeth and Somers, Anthony and Forsyth, Maria and Machuca, Laura L
Journal: Microorganisms (2022): 1285
A viral ubiquitination switch attenuates innate immunity and triggers nuclear import of virion DNA and infection
Authors: Bauer, Michael and Gomez-Gonzalez, Alfonso and Suomalainen, Maarit and Schilling, Nicolas and Hemmi, Silvio and Greber, Urs F
Journal: Science advances (2021): eabl7150
Non-viral gene delivery of the oncotoxic protein NS1 for treatment of hepatocellular carcinoma
Authors: Witzigmann, Dominik and Grossen, Philip and Quintavalle, Cristina and Lanzafame, Manuela and Schenk, Susanne H and Tran, Xue-Ting and Englinger, Bernhard and Hauswirth, Patrick and Gr{\"u}nig, David and van Schoonhoven, Sushilla and others,
Journal: Journal of Controlled Release (2021): 138--152
DNase-mediated eDNA removal enhances D-LL-31 activity against biofilms of bacteria isolated from chronic rhinosinusitis patients
Authors: Wongkaewkhiaw, Saharut and Kanthawong, Sakawrat and Bolscher, Jan GM and Nazmi, Kamran and Taweechaisupapong, Suwimol and Krom, Bastiaan P
Journal: Biofouling (2020): 1--12

References

View all 22 references: Citation Explorer
Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524
Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
Page updated on October 12, 2024

Ordering information

Price
Unit size
Catalog Number17579
Quantity
Add to cart

Additional ordering information

Telephone1-800-990-8053
Fax1-800-609-2943
Emailsales@aatbio.com
InternationalSee distributors
Bulk requestInquire
Custom sizeInquire
Technical SupportContact us
Purchase orderSend to sales@aatbio.com
ShippingStandard overnight for United States, inquire for international
Request quotation

Physical properties

Molecular weight

1270.65

Solvent

DMSO

Spectral properties

Extinction coefficient (cm -1 M -1)

989001

Excitation (nm)

491

Emission (nm)

508

Quantum yield

0.521

Storage, safety and handling

H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC41116134

CAS

143413-85-8
Product Image
Product Image
Gallery Image 1