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DiYO™-1 [equivalent to YOYO®-1]

Chemical structure for DiYO™-1 [equivalent to YOYO®-1].
Chemical structure for DiYO™-1 [equivalent to YOYO®-1].
Ordering information
Price ()
Catalog Number17579
Unit Size
Find Distributor
Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight1270.65
SolventWater
Spectral properties
Extinction coefficient (cm -1 M -1)989001
Excitation (nm)491
Emission (nm)508
Quantum yield0.521
Storage, safety and handling
H-phraseH303, H313, H340
Hazard symbolT
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R68
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC41116134

OverviewpdfSDSpdfProtocol


CAS
143413-85-8
Molecular weight
1270.65
Extinction coefficient (cm -1 M -1)
989001
Excitation (nm)
491
Emission (nm)
508
Quantum yield
0.521
DiYO™-1 is chemically equivalent to YOYO®-1 (YOYO® is the trademark of Invitrogen). DiYO™-1 is a carbocyanine dimer with green fluorescence similar to FITC. It is cell-impermeant and easily distinguished from Cy5 and rhodamines as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiYO™-1 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Red™ LCS1 dye and cell-impermeant DiYO®-1 can be used to assess cell viability. DiYO™-1 and Nuclear Red™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.

Calculators


Common stock solution preparation

Table 1. Volume of Water needed to reconstitute specific mass of DiYO™-1 [equivalent to YOYO®-1] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM78.7 µL393.499 µL786.999 µL3.935 mL7.87 mL
5 mM15.74 µL78.7 µL157.4 µL786.999 µL1.574 mL
10 mM7.87 µL39.35 µL78.7 µL393.499 µL786.999 µL

Molarity calculator

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Spectrum


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spectrum

Spectral properties

Extinction coefficient (cm -1 M -1)989001
Excitation (nm)491
Emission (nm)508
Quantum yield0.521

Citations


View all 25 citations: Citation Explorer
Non-viral gene delivery of the oncotoxic protein NS1 for treatment of hepatocellular carcinoma
Authors: Witzigmann, Dominik and Grossen, Philip and Quintavalle, Cristina and Lanzafame, Manuela and Schenk, Susanne H and Tran, Xue-Ting and Englinger, Bernhard and Hauswirth, Patrick and Gr{\"u}nig, David and van Schoonhoven, Sushilla and others,
Journal: Journal of Controlled Release (2021): 138--152
A viral ubiquitination switch attenuates innate immunity and triggers nuclear import of virion DNA and infection
Authors: Bauer, Michael and Gomez-Gonzalez, Alfonso and Suomalainen, Maarit and Schilling, Nicolas and Hemmi, Silvio and Greber, Urs F
Journal: Science advances (2021): eabl7150
DNase-mediated eDNA removal enhances D-LL-31 activity against biofilms of bacteria isolated from chronic rhinosinusitis patients
Authors: Wongkaewkhiaw, Saharut and Kanthawong, Sakawrat and Bolscher, Jan GM and Nazmi, Kamran and Taweechaisupapong, Suwimol and Krom, Bastiaan P
Journal: Biofouling (2020): 1--12
Correction: Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging
Authors: Pyle, J. R., Chen, J.
Journal: Beilstein J Nanotechnol (2018): 809-811
Ultrafast Transient Absorption Spectra of Photoexcited YOYO-1 molecules call for additional investigations of their fluorescence quenching mechanism
Authors: Wang, L., Pyle, J. R., Cimatu, K. A., Chen, J.
Journal: J Photochem Photobiol A Chem (2018): 411-419
Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging
Authors: Pyle, J. R., Chen, J.
Journal: Beilstein J Nanotechnol (2017): 2296-2306
Detection of amyloid-beta fibrils using the DNA-intercalating dye YOYO-1: Binding mode and fibril formation kinetics
Authors: Lindberg, D. J., Esbjorner, E. K.
Journal: Biochem Biophys Res Commun (2016): 313-8
Charging YOYO-1 on capillary wall for online DNA intercalation and integrating this approach with multiplex PCR and bare narrow capillary-hydrodynamic chromatography for online DNA analysis
Authors: Chen, H., Zhu, Z., Lu, J. J., Liu, S.
Journal: Anal Chem (2015): 1518-22
Interference of ATP with the fluorescent probes YOYO-1 andYOYO-3 modifies the mechanical properties of intercalator-stained DNA confined in nanochannels
Authors: Roushan, M., Azad, Z., Lim, S. F., Wang, H., Riehn, R.
Journal: Mikrochim Acta (2015): 1561-1565
Interaction of YOYO-1 with guanine-rich DNA
Authors: Datta, S. G., Reynolds, C., Goud, Y. K., Datta, B.
Journal: J Biomol Struct Dyn (2014): 1155-63

References


View all 22 references: Citation Explorer
Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524
Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630
Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713
Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29
Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87
DNA length evaluation using cyanine dye and fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Tsuruoka M.
Journal: Biomacromolecules (2005): 2703
Implementation of accurate and fast DNA cytometry by confocal microscopy in 3D
Authors: Ploeger LS, Huisman A, van der Gugten J, van der Giezen DM, Belien JA, Abbaker AY, Dullens HF, Grizzle W, Poulin NM, Meijer GA, van Diest PJ.
Journal: Cell Oncol (2005): 225
TO-PRO-3 is an optimal fluorescent dye for nuclear counterstaining in dual-colour FISH on paraffin sections
Authors: Bink K, Walch A, Feuchtinger A, Eisenmann H, Hutzler P, Hofler H, Werner M.
Journal: Histochem Cell Biol (2001): 293
Oxazole yellow homodimer YOYO-1-labeled DNA: a fluorescent complex that can be used to assess structural changes in DNA following formation and cellular delivery of cationic lipid DNA complexes
Authors: Wong M, Kong S, Dragowska WH, Bally MB.
Journal: Biochim Biophys Acta (2001): 61
Photophysical properties of fluorescent DNA-dyes bound to single- and double-stranded DNA in aqueous buffered solution
Authors: Cosa G, Focsaneanu KS, McLean JR, McNamee JP, Scaiano JC.
Journal: Photochem Photobiol (2001): 585