DiYO™-1 [equivalent to YOYO®-1]

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Additional ordering information

Telephone	1-800-990-8053
Fax	1-800-609-2943
Email	sales@aatbio.com
International	See distributors
Bulk request	Inquire
Custom size	Inquire
Shipping	Standard overnight for United States, inquire for international

Physical properties

Molecular weight	1270.65
Solvent	DMSO

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	98900¹
Excitation (nm)	491
Emission (nm)	508
Quantum yield	0.52¹

Storage, safety and handling

H-phrase	H303, H313, H340
Hazard symbol	T
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R68
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	41116134

Alternative formats

DiYO™-1 [equivalent to YOYO®-1] *5 mM DMSO Solution*

Overview SDS Protocol

CAS

143413-85-8

Molecular weight

1270.65

Extinction coefficient (cm ^-1 M ^-1)

98900¹

Excitation (nm)

491

Emission (nm)

508

Quantum yield

0.52¹

DiYO™-1 is chemically equivalent to YOYO®-1 (YOYO® is the trademark of Invitrogen). DiYO™-1 is a carbocyanine dimer with green fluorescence similar to FITC. It is cell-impermeant and easily distinguished from Cy5 and rhodamines as a nuclear counterstain and dead cell indicator. It is non-fluorescent in the absence of nucleic acids, and generates a very bright fluorescence signal upon binding to DNA. DiYO™-1 gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with cell-permeable Nuclear Red™ LCS1 dye and cell-impermeant DiYO®-1 can be used to assess cell viability. DiYO™-1 and Nuclear Red™ both have much greater extinction coefficients than that of DNA-bound propidium iodide.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DiYO™-1 [equivalent to YOYO®-1] to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	78.7 µL	393.499 µL	786.999 µL	3.935 mL	7.87 mL
5 mM	15.74 µL	78.7 µL	157.4 µL	786.999 µL	1.574 mL
10 mM	7.87 µL	39.35 µL	78.7 µL	393.499 µL	786.999 µL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
	/		=		x		=

Spectrum

Open in Advanced Spectrum Viewer

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	98900¹
Excitation (nm)	491
Emission (nm)	508
Quantum yield	0.52¹

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)
DiYO™-3 [equivalent to YOYO®-3] 5 mM DMSO Solution CAS 156312-20-8	612	631	167000¹
Oxazine 1 CAS 24796-94-9	648	668	123000
DiTO™-1 [equivalent to TOTO®-1] 5 mM DMSO Solution CAS 143413-84-7	514	531	117000¹
JC-1 [5,5,6,6-Tetrachloro-1,1,3,3-tetraethylbenzimidazolylcarbocyanine iodide] CAS#: 3520-43-2	515	530	195000¹
BTA-1 [2-(4'-(methylamino)phenyl)benzothiazole]	349	421	-
DiPO™-1 [equivalent to POPO®-1] 1 mM DMSO Solution	433	457	92000¹
EthD-1 [Ethidium Homodimer-1]	528	617	-

Images

Figure 1. Chemical structure for DiYO™-1 [equivalent to YOYO®-1].

Citations

View all 27 citations: Citation Explorer

Trellis tree-based analysis reveals stromal regulation of patient-derived organoid drug responses
Authors: Zapatero, Mar{\'\i}a Ramos and Tong, Alexander and Opzoomer, James W and O’Sullivan, Rhianna and Rodriguez, Ferran Cardoso and Sufi, Jahangir and Vlckova, Petra and Nattress, Callum and Qin, Xiao and Claus, Jeroen and others,
Journal: Cell (2023): 5606--5619

Extracellular DNA: A Critical Aspect of Marine Biofilms
Authors: Tuck, Benjamin and Salgar-Chaparro, Silvia J and Watkin, Elizabeth and Somers, Anthony and Forsyth, Maria and Machuca, Laura L
Journal: Microorganisms (2022): 1285

Non-viral gene delivery of the oncotoxic protein NS1 for treatment of hepatocellular carcinoma
Authors: Witzigmann, Dominik and Grossen, Philip and Quintavalle, Cristina and Lanzafame, Manuela and Schenk, Susanne H and Tran, Xue-Ting and Englinger, Bernhard and Hauswirth, Patrick and Gr{\"u}nig, David and van Schoonhoven, Sushilla and others,
Journal: Journal of Controlled Release (2021): 138--152

A viral ubiquitination switch attenuates innate immunity and triggers nuclear import of virion DNA and infection
Authors: Bauer, Michael and Gomez-Gonzalez, Alfonso and Suomalainen, Maarit and Schilling, Nicolas and Hemmi, Silvio and Greber, Urs F
Journal: Science advances (2021): eabl7150

DNase-mediated eDNA removal enhances D-LL-31 activity against biofilms of bacteria isolated from chronic rhinosinusitis patients
Authors: Wongkaewkhiaw, Saharut and Kanthawong, Sakawrat and Bolscher, Jan GM and Nazmi, Kamran and Taweechaisupapong, Suwimol and Krom, Bastiaan P
Journal: Biofouling (2020): 1--12

Correction: Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging
Authors: Pyle, J. R., Chen, J.
Journal: Beilstein J Nanotechnol (2018): 809-811

Ultrafast Transient Absorption Spectra of Photoexcited YOYO-1 molecules call for additional investigations of their fluorescence quenching mechanism
Authors: Wang, L., Pyle, J. R., Cimatu, K. A., Chen, J.
Journal: J Photochem Photobiol A Chem (2018): 411-419

Photobleaching of YOYO-1 in super-resolution single DNA fluorescence imaging
Authors: Pyle, J. R., Chen, J.
Journal: Beilstein J Nanotechnol (2017): 2296-2306

Detection of amyloid-beta fibrils using the DNA-intercalating dye YOYO-1: Binding mode and fibril formation kinetics
Authors: Lindberg, D. J., Esbjorner, E. K.
Journal: Biochem Biophys Res Commun (2016): 313-8

Charging YOYO-1 on capillary wall for online DNA intercalation and integrating this approach with multiplex PCR and bare narrow capillary-hydrodynamic chromatography for online DNA analysis
Authors: Chen, H., Zhu, Z., Lu, J. J., Liu, S.
Journal: Anal Chem (2015): 1518-22

References

View all 22 references: Citation Explorer

Plasmalemma permeability and necrotic cell death phenotypes after intracerebral hemorrhage in mice
Authors: Zhu X, Tao L, Tejima-M and eville E, Qiu J, Park J, Garber K, Ericsson M, Lo EH, Whalen MJ.
Journal: Stroke (2012): 524

Intra-organ Biodistribution of Gold Nanoparticles Using Intrinsic Two-photon Induced Photoluminescence
Authors: Park J, Estrada A, Schwartz JA, Diagaradjane P, Krishnan S, Dunn AK, Tunnell JW.
Journal: Lasers Surg Med (2010): 630

Screening by imaging: scaling up single-DNA-molecule analysis with a novel parabolic VA-TIRF reflector and noise-reduction techniques
Authors: van 't Hoff M, Reuter M, Dryden DT, Oheim M.
Journal: Phys Chem Chem Phys (2009): 7713

Novel anatomic structures in the brain and spinal cord of rabbit that may belong to the Bonghan system of potential acupuncture meridians
Authors: Lee BC, Kim S, Soh KS.
Journal: J Acupunct Meridian Stud (2008): 29

Triplet fraction buildup effect of the DNA-YOYO complex studied with fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Kinjo M.
Journal: Anal Biochem (2007): 87

DNA length evaluation using cyanine dye and fluorescence correlation spectroscopy
Authors: Shimizu M, Sasaki S, Tsuruoka M.
Journal: Biomacromolecules (2005): 2703

Implementation of accurate and fast DNA cytometry by confocal microscopy in 3D
Authors: Ploeger LS, Huisman A, van der Gugten J, van der Giezen DM, Belien JA, Abbaker AY, Dullens HF, Grizzle W, Poulin NM, Meijer GA, van Diest PJ.
Journal: Cell Oncol (2005): 225

TO-PRO-3 is an optimal fluorescent dye for nuclear counterstaining in dual-colour FISH on paraffin sections
Authors: Bink K, Walch A, Feuchtinger A, Eisenmann H, Hutzler P, Hofler H, Werner M.
Journal: Histochem Cell Biol (2001): 293

Oxazole yellow homodimer YOYO-1-labeled DNA: a fluorescent complex that can be used to assess structural changes in DNA following formation and cellular delivery of cationic lipid DNA complexes
Authors: Wong M, Kong S, Dragowska WH, Bally MB.
Journal: Biochim Biophys Acta (2001): 61

Photophysical properties of fluorescent DNA-dyes bound to single- and double-stranded DNA in aqueous buffered solution
Authors: Cosa G, Focsaneanu KS, McLean JR, McNamee JP, Scaiano JC.
Journal: Photochem Photobiol (2001): 585