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DNP amine

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Physical properties
Molecular weight226.19
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Alternative formats
DNP maleimide

OverviewpdfSDSpdfProtocol


Molecular weight
226.19
A carbonyl-reactive building block for developing a probe that can be recognized by anti-DNP antibodies; Also an excellent carbonyl-reactive FRET quencher paired with Trp or Tyr.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DNP amine to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM442.106 µL2.211 mL4.421 mL22.105 mL44.211 mL
5 mM88.421 µL442.106 µL884.212 µL4.421 mL8.842 mL
10 mM44.211 µL221.053 µL442.106 µL2.211 mL4.421 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Product Family


NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)
ICG amine789813230000-0.076
XFD488 amine *Same Structure to Alexa Fluor™ 488 amine*499520710000.9210.11
XFD594 amine *Same Structure to Alexa Fluor™ 594 amine*590618900000.6610.56
XFD532 amine534553810000.6110.09
XFD555 amine5535681550000.110.08
XFD568 amine579603880000.6910.46
XFD647 amine6506712700000.3310.03
XFD750 amine7527762900000.1210.04

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References


View all 124 references: Citation Explorer
Continuous assay of protein tyrosine phosphatases based on fluorescence resonance energy transfer
Authors: Nishikata, M.; Yoshimura, Y.; Deyama, Y.; Suzuki, K.
Journal: Biochimie (2006): 879-86
High-throughput screening of low molecular weight NS3-NS4A protease inhibitors using a fluorescence resonance energy transfer substrate
Authors: Sudo K, Yamaji K, Kawamura K, Nishijima T, Kojima N, Aibe K, Shimotohno K, Shimizu Y.
Journal: Antivir Chem Chemother (2005): 385
Recombinant human cathepsin X is a carboxymonopeptidase only: a comparison with cathepsins B and L
Authors: Puzer L, Cotrin SS, Cezari MH, Hirata IY, Juliano MA, Stefe I, Turk D, Turk B, Juliano L, Carmona AK.
Journal: Biol Chem (2005): 1191
Evaluation of an imaging platform during the development of a FRET protease assay
Authors: George J, Teear ML, Norey CG, Burns DD.
Journal: J Biomol Screen (2003): 72
Generation of heterogeneous rabbit anti-DNP antibodies by gene conversion and hypermutation of rearranged VL and VH genes during clonal expansion of B cells in splenic germinal centers
Authors: Sehgal D, Schiaffella E, Anderson AO, Mage RG.
Journal: Eur J Immunol (2000): 3634
Effects and mechanisms of environmental temperature on carp (Cyprinus carpio) anti-DNP antibody response and non-specific cytotoxic cell activity: a kinetic study
Authors: Le Morvan C, Deschaux P, Troutaud D.
Journal: Dev Comp Immunol (1996): 331
Determination of the binding of ligands containing the N-2,4-dinitrophenyl group to bivalent monoclonal rat anti-DNP antibody using affinity capillary electrophoresis
Authors: Mammen M, Gomez FA, Whitesides GM.
Journal: Anal Chem (1995): 3526
Effects of Celosia argentea and Cucurbita moschata extracts on anti-DNP IgE antibody production in mice
Authors: Imaoka K, Ushijima H, Inouye S, Takahashi T, Kojima Y.
Journal: Arerugi (1994): 652
Effects of Perilla frutescens extract on anti-DNP IgE antibody production in mice
Authors: Imaoka K, Inouye S, Takahashi T, Kojima Y.
Journal: Arerugi (1993): 74
Effect of anti-DNP IgG1- and IgG2a-secreting hybridomas in vivo on the development of an anti-DNP IgE antibody response in mice
Authors: Hagen M, Morrison B, Robbinson D, Strejan GH.
Journal: Int Arch Allergy Immunol (1992): 146