DNP maleimide

A thiol-reactive building block for developing a probe that can be recognized by anti-DNP antibodies; Also an excellent thiol-reactive FRET quencher paired with Trp or Tyr.

Protocol

SDS

COA

Catalog	Size	Price	Quantity
2026	25 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DNP maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	326.552 µL	1.633 mL	3.266 mL	16.328 mL	32.655 mL
5 mM	65.31 µL	326.552 µL	653.104 µL	3.266 mL	6.531 mL
10 mM	32.655 µL	163.276 µL	326.552 µL	1.633 mL	3.266 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	306.23 g/mol	=	mL	x	mM	=	mmol

Citations

View all 2 citations: Citation Explorer

Characterization of Hydrogel Drug Delivery System Stability Using Column-Switching Multimodal Liquid Chromatography--Mass Spectrometry

Authors:

Drennan, Brady W and Dinh, Phat and Schug, Kevin A and Zhang, Kelly and Yang, Samuel H

Journal:

Analytical Chemistry (2025)

Imaging of extracellular cathepsin S activity by a selective near infrared fluorescence substrate-based probe

Authors:

Wartenberg, Mylène and Saidi, Ahlame and Galibert, Mathieu and Joulin-Giet, Alix and Burlaud-Gaillard, Julien and Lecaille, Fabien and Scott, Christopher J and Aucagne, Vincent and Delmas, Agnès F and Lalmanach, Gilles

Journal:

Biochimie (2019)

References

View all 124 references: Citation Explorer

Continuous assay of protein tyrosine phosphatases based on fluorescence resonance energy transfer

Authors:

Nishikata, M.; Yoshimura, Y.; Deyama, Y.; Suzuki, K.

Journal:

Biochimie (2006): 879-86

High-throughput screening of low molecular weight NS3-NS4A protease inhibitors using a fluorescence resonance energy transfer substrate

Authors:

Sudo K, Yamaji K, Kawamura K, Nishijima T, Kojima N, Aibe K, Shimotohno K, Shimizu Y.

Journal:

Antivir Chem Chemother (2005): 385

Recombinant human cathepsin X is a carboxymonopeptidase only: a comparison with cathepsins B and L

Authors:

Puzer L, Cotrin SS, Cezari MH, Hirata IY, Juliano MA, Stefe I, Turk D, Turk B, Juliano L, Carmona AK.

Journal:

Biol Chem (2005): 1191

Evaluation of an imaging platform during the development of a FRET protease assay

Authors:

George J, Teear ML, Norey CG, Burns DD.

Journal:

J Biomol Screen (2003): 72

Generation of heterogeneous rabbit anti-DNP antibodies by gene conversion and hypermutation of rearranged VL and VH genes during clonal expansion of B cells in splenic germinal centers

Authors:

Sehgal D, Schiaffella E, Anderson AO, Mage RG.

Journal:

Eur J Immunol (2000): 3634

Physical properties

Molecular weight	306.23
Solvent	DMSO

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12352200
CAS	62024-74-2

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Page updated on October 12, 2025