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DNP maleimide

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Physical properties
Molecular weight306.23
SolventDMSO
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Alternative formats
DNP amine

OverviewpdfSDSpdfProtocol


CAS
62024-74-2
Molecular weight
306.23
A thiol-reactive building block for developing a probe that can be recognized by anti-DNP antibodies; Also an excellent thiol-reactive FRET quencher paired with Trp or Tyr.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of DNP maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM326.552 µL1.633 mL3.266 mL16.328 mL32.655 mL
5 mM65.31 µL326.552 µL653.104 µL3.266 mL6.531 mL
10 mM32.655 µL163.276 µL326.552 µL1.633 mL3.266 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Citations


View all 1 citations: Citation Explorer
Imaging of extracellular cathepsin S activity by a selective near infrared fluorescence substrate-based probe
Authors: Wartenberg, Myl&egrave;ne and Saidi, Ahlame and Galibert, Mathieu and Joulin-Giet, Alix and Burlaud-Gaillard, Julien and Lecaille, Fabien and Scott, Christopher J and Aucagne, Vincent and Delmas, Agn&egrave;s F and Lalmanach, Gilles
Journal: Biochimie (2019)

References


View all 124 references: Citation Explorer
Continuous assay of protein tyrosine phosphatases based on fluorescence resonance energy transfer
Authors: Nishikata, M.; Yoshimura, Y.; Deyama, Y.; Suzuki, K.
Journal: Biochimie (2006): 879-86
High-throughput screening of low molecular weight NS3-NS4A protease inhibitors using a fluorescence resonance energy transfer substrate
Authors: Sudo K, Yamaji K, Kawamura K, Nishijima T, Kojima N, Aibe K, Shimotohno K, Shimizu Y.
Journal: Antivir Chem Chemother (2005): 385
Recombinant human cathepsin X is a carboxymonopeptidase only: a comparison with cathepsins B and L
Authors: Puzer L, Cotrin SS, Cezari MH, Hirata IY, Juliano MA, Stefe I, Turk D, Turk B, Juliano L, Carmona AK.
Journal: Biol Chem (2005): 1191
Evaluation of an imaging platform during the development of a FRET protease assay
Authors: George J, Teear ML, Norey CG, Burns DD.
Journal: J Biomol Screen (2003): 72
Generation of heterogeneous rabbit anti-DNP antibodies by gene conversion and hypermutation of rearranged VL and VH genes during clonal expansion of B cells in splenic germinal centers
Authors: Sehgal D, Schiaffella E, Anderson AO, Mage RG.
Journal: Eur J Immunol (2000): 3634
Effects and mechanisms of environmental temperature on carp (Cyprinus carpio) anti-DNP antibody response and non-specific cytotoxic cell activity: a kinetic study
Authors: Le Morvan C, Deschaux P, Troutaud D.
Journal: Dev Comp Immunol (1996): 331
Determination of the binding of ligands containing the N-2,4-dinitrophenyl group to bivalent monoclonal rat anti-DNP antibody using affinity capillary electrophoresis
Authors: Mammen M, Gomez FA, Whitesides GM.
Journal: Anal Chem (1995): 3526
Effects of Celosia argentea and Cucurbita moschata extracts on anti-DNP IgE antibody production in mice
Authors: Imaoka K, Ushijima H, Inouye S, Takahashi T, Kojima Y.
Journal: Arerugi (1994): 652
Effects of Perilla frutescens extract on anti-DNP IgE antibody production in mice
Authors: Imaoka K, Inouye S, Takahashi T, Kojima Y.
Journal: Arerugi (1993): 74
Effect of anti-DNP IgG1- and IgG2a-secreting hybridomas in vivo on the development of an anti-DNP IgE antibody response in mice
Authors: Hagen M, Morrison B, Robbinson D, Strejan GH.
Journal: Int Arch Allergy Immunol (1992): 146