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DNP-PEG4 acid
Ordering information
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Additional ordering information
| Telephone | 1-800-990-8053 |
| Fax | 1-800-609-2943 |
| sales@aatbio.com | |
| International | See distributors |
| Bulk request | Inquire |
| Custom size | Inquire |
| Shipping | Standard overnight for United States, inquire for international |
Physical properties
| Molecular weight | 431.40 |
| Solvent | DMSO |
Storage, safety and handling
| H-phrase | H303, H313, H333 |
| Hazard symbol | XN |
| Intended use | Research Use Only (RUO) |
| R-phrase | R20, R21, R22 |
| Storage | Freeze (< -15 °C); Minimize light exposure |
| UNSPSC | 12171501 |
| Overview |  SDSProtocol |
CAS 858126-76-8 | Molecular weight 431.40 |
Compared to the commonly used DNP-X acid (#2020), DNP-PEG4 acid has much better water solubility. It is an excellent amine-reactive building block for developing a probe that can be recognized by anti-DNP antibodies. It might be also used as an excellent amine-reactive FRET quencher paired with Trp or Tyr. Its terminal carboxy group readily reacts with primary amines in the presence of EDC and DCC to form stable amide bonds. The hydrophilic PEG linker increases the water solubility of the compound in aqueous media.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of DNP-PEG4 acid to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
| 0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
| 1 mM | 231.803 µL | 1.159 mL | 2.318 mL | 11.59 mL | 23.18 mL |
| 5 mM | 46.361 µL | 231.803 µL | 463.607 µL | 2.318 mL | 4.636 mL |
| 10 mM | 23.18 µL | 115.902 µL | 231.803 µL | 1.159 mL | 2.318 mL |
Molarity calculator
Enter any two values (mass, volume, concentration) to calculate the third.
| Mass (Calculate) | Molecular weight | Volume (Calculate) | Concentration (Calculate) | Moles | ||||
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Images
Figure 1. Chemical structure for DNP-PEG4 acid.
References
View all 50 references: Citation Explorer
Nano-Snowflower of Gold Nanoparticles-Ruthenium Metallopolymer-Carbon Nanotubes Binding Anti-DNP IgE Antibody.
Authors: Li, Huayang and Wu, Jie and Melnyczuk, John M and Olubi, Omotunde and Lewis, Lauchon I and Cao, Yang and Nagappan, Peri and Khan, Shafiq A and Ingram, Conrad W and Harruna, Issifu I
Journal: Journal of nanoscience and nanotechnology (2015): 5733-40
Authors: Li, Huayang and Wu, Jie and Melnyczuk, John M and Olubi, Omotunde and Lewis, Lauchon I and Cao, Yang and Nagappan, Peri and Khan, Shafiq A and Ingram, Conrad W and Harruna, Issifu I
Journal: Journal of nanoscience and nanotechnology (2015): 5733-40
Peptide mimics of hapten DNP: the effect of affinity of anti-DNP monoclonal antibodies for the selection of phage-displayed mimotopes.
Authors: Kalaycioglu, Atila T and Russell, Peter H and Howard, Colin R
Journal: Journal of immunological methods (2011): 36-42
Authors: Kalaycioglu, Atila T and Russell, Peter H and Howard, Colin R
Journal: Journal of immunological methods (2011): 36-42
Generation of heterogeneous rabbit anti-DNP antibodies by gene conversion and hypermutation of rearranged VL and VH genes during clonal expansion of B cells in splenic germinal centers.
Authors: Sehgal, D and Schiaffella, E and Anderson, A O and Mage, R G
Journal: European journal of immunology (2000): 3634-44
Authors: Sehgal, D and Schiaffella, E and Anderson, A O and Mage, R G
Journal: European journal of immunology (2000): 3634-44
Determination of the binding of ligands containing the N-2,4-dinitrophenyl group to bivalent monoclonal rat anti-DNP antibody using affinity capillary electrophoresis.
Authors: Mammen, M and Gomez, F A and Whitesides, G M
Journal: Analytical chemistry (1995): 3526-35
Authors: Mammen, M and Gomez, F A and Whitesides, G M
Journal: Analytical chemistry (1995): 3526-35
Fast solid support detection of human papillomavirus in in vitro amplified DNA using a DNP-anti DNP monoclonal antibody couple.
Authors: Baccard-Longère, M and Alpha-Bazin, B and Chypre, C and Sauvaigo, S and Téoule, R and Bernard, P and Seigneurin, J M
Journal: Journal of virological methods (1994): 29-38
Authors: Baccard-Longère, M and Alpha-Bazin, B and Chypre, C and Sauvaigo, S and Téoule, R and Bernard, P and Seigneurin, J M
Journal: Journal of virological methods (1994): 29-38
[Effects of Celosia argentea and Cucurbita moschata extracts on anti-DNP IgE antibody production in mice].
Authors: Imaoka, K and Ushijima, H and Inouye, S and Takahashi, T and Kojima, Y
Journal: Arerugi = [Allergy] (1994): 652-9
Authors: Imaoka, K and Ushijima, H and Inouye, S and Takahashi, T and Kojima, Y
Journal: Arerugi = [Allergy] (1994): 652-9
[Effects of Perilla frutescens extract on anti-DNP IgE antibody production in mice].
Authors: Imaoka, K and Inouye, S and Takahashi, T and Kojima, Y
Journal: Arerugi = [Allergy] (1993): 74-80
Authors: Imaoka, K and Inouye, S and Takahashi, T and Kojima, Y
Journal: Arerugi = [Allergy] (1993): 74-80
Effect of anti-DNP IgG1- and IgG2a-secreting hybridomas in vivo on the development of an anti-DNP IgE antibody response in mice.
Authors: Hagen, M and Morrison, B and Robbinson, D and Strejan, G H
Journal: International archives of allergy and immunology (1992): 146-53
Authors: Hagen, M and Morrison, B and Robbinson, D and Strejan, G H
Journal: International archives of allergy and immunology (1992): 146-53
Mechanism of allergic cross-reactions--I. Multispecific binding of ligands to a mouse monoclonal anti-DNP IgE antibody.
Authors: Varga, J M and Kalchschmid, G and Klein, G F and Fritsch, P
Journal: Molecular immunology (1991): 641-54
Authors: Varga, J M and Kalchschmid, G and Klein, G F and Fritsch, P
Journal: Molecular immunology (1991): 641-54
Mechanism of allergic cross-reactions--II. Cross-stimulation, by chemically unrelated ligands, of rat basophilic leukemia cells sensitized with an anti-DNP IgE antibody.
Authors: Varga, J M and Kalchschmid, G and Klein, G F and Fritsch, P
Journal: Molecular immunology (1991): 655-9
Authors: Varga, J M and Kalchschmid, G and Klein, G F and Fritsch, P
Journal: Molecular immunology (1991): 655-9