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Droplite™ Green
Lipid Droplets (LDs) are essential organelles responsible for storing neutral lipids, primarily consisting of triglycerides and cholesterol esters. Found in a variety of cell types, LDs play crucial roles in numerous biological processes, including metabolism, membrane biosynthesis, cell signaling, inflammation, and cancer-related mechanisms. Droplite™ Green, developed by AAT Bioquest, is a green fluorescent dye with an extremely high affinity for LDs and minimal cell toxicity. This probe has an excitation and emission maxima of 421 nm and 521 nm, respectively, and can be conveniently detected using fluorescence microscopy or an HCS reader. In cell culture experiments, Droplite™ Green demonstrates exceptional safety, even at high concentrations, ensuring reliable and accurate LD characterization without compromising cell health and viability.
Fluorescence images of intracellular lipid droplets in control (left) and Oleic Acid treated HeLa cells (right) using Droplite™ Green. HeLa cells were incubated with 100 uM of Oleic Acid for 19 hours to induce intracellular lipid droplet formation. After washing with DPBS, HHBS was added to the cells, and images were acquired with a fluorescence microscope using a FITC filter set.
Fluorescence images of intracellular lipid droplets in control (left) and Oleic Acid treated HeLa cells (right) using Droplite™ Green. HeLa cells were incubated with 100 uM of Oleic Acid for 19 hours to induce intracellular lipid droplet formation. After washing with DPBS, HHBS was added to the cells, and images were acquired with a fluorescence microscope using a FITC filter set.
protocol
Protocol
sds
SDS
COA
CatalogSize
Price
Quantity
22729100 Tests
Price
 
Physical properties
Molecular weight432.37
SolventDMSO
Spectral properties
Excitation (nm)421
Emission (nm)521
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12352200
Instrument settings
Fluorescence microscope
ExcitationFITC filter set
EmissionFITC filter set
Recommended plateBlack wall/clear bottom
Instrument specification(s)FITC filter set
Documents
Protocol
Safety Data Sheet
Certificate of Analysis
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Page updated on October 27, 2025