Name: DY547 CEP
Brand: AAT Bioquest
SKU: 6074
Price: 538 USD
Availability: InStock

DY547 CEP

DY547 CEP (DyLight 547 phosphoramidite) is an excellent replacement to the commonly used Cy3 phosphoramidite. Two cyanine derivatives, Cy3 and Cy5 phosphoramidites, are the most popular fluorescent labeling reagents for preparing fluorescence-labeled oligonucleotides. However, both Cy3 and Cy5 phosphoramidites are not very stable. DY547 CEP appears to be more stable than Cy3 phosphoramidite. As Cy3 and Cy5 phosphoramidites, the deprotection of oligos containing DY547 CEP may be carried out with ammonium hydroxide at room temperature, regardless of the base protecting groups on the monomers used. However, it is always prudent to use monomers with base labile protecting groups to limit the exposure time to 2 hours or less at 65°C during deprotection.

Datasheet

SDS

Catalog	Size	Price	Quantity
6074	100 umoles	Price

Calculators

Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of DY547 CEP to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	132.144 µL	660.72 µL	1.321 mL	6.607 mL	13.214 mL
5 mM	26.429 µL	132.144 µL	264.288 µL	1.321 mL	2.643 mL
10 mM	13.214 µL	66.072 µL	132.144 µL	660.72 µL	1.321 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	756.75 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Probing functional conformation-state fluctuation dynamics in recognition binding between calmodulin and target peptide.

Authors:

Jaiswal, Sunidhi and He, Yufan and Lu, H Peter

Journal:

The Journal of chemical physics (2022): 055102

Insulin-Like Growth Factor-1 Receptor Targeted Fluorescent Imaging for Gallbladder Cancer in Orthotopic Mouse Models.

Authors:

Choi, Jung Ha and Park, Jeong Youp

Journal:

Gut and liver (2021)

Stability Enhancement of a Dimeric HER2-Specific Affibody Molecule through Sortase A-Catalyzed Head-to-Tail Cyclization.

Authors:

Westerlund, Kristina and Myrhammar, Anders and Tano, Hanna and Gestin, Maxime and Karlström, Amelie Eriksson

Journal:

Molecules (Basel, Switzerland) (2021)

Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems.

Authors:

Stech, Marlitt and Rakotoarinoro, Nathanaël and Teichmann, Tamara and Zemella, Anne and Thoring, Lena and Kubick, Stefan

Journal:

Methods in molecular biology (Clifton, N.J.) (2021): 175-190

Novel EBV LMP1 C-terminal domain binding affibody molecules as potential agents for in vivo molecular imaging diagnosis of nasopharyngeal carcinoma.

Authors:

Kamara, Saidu and Guo, Yanru and Mao, Shanshan and Ye, Xiaoxian and Li, Qingfeng and Zheng, Maolin and Zhu, Jinshun and Zhang, Jing and Du, Wangqi and Chen, Jun and Zhu, Shanli and Zhang, Lifang

Journal:

Applied microbiology and biotechnology (2021): 7283-7293

Physical properties

Molecular weight	756.75
Solvent	MeCN

Spectral properties

Extinction coefficient (cm ^-1 M ^-1)	150000 ¹
Excitation (nm)	558
Emission (nm)	574

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

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Page updated on May 1, 2026