DY677 CEP | AAT Bioquest

DY677 CEP

DY677 CEP (DyLight 677 phosphoramidite) is an excellent replacement to the commonly used Cy5.5 phosphoramidite. Cyanine phosphoramidites (such as Cy3 and Cy5 phosphoramidites) are the most popular fluorescent labeling reagents for preparing fluorescence-labeled oligonucleotides. However, both Cy3 and Cy5 phosphoramidites are not very stable. DY677 CEP appears to be more stable than Cy5.5 phosphoramidite. As Cy5.5 phosphoramidite, the deprotection of oligos containing DY677 CEP may be carried out with ammonium hydroxide at room temperature, regardless of the base protecting groups on the monomers used. However, it is always prudent to use monomers with base labile protecting groups to limit the exposure time to 2 hours or less at 65°C during deprotection.

Datasheet

SDS

Catalog	Size	Price	Quantity
6078	100 umoles	Price

Calculators

Common stock solution preparation

Table 1. Volume of MeCN needed to reconstitute specific mass of DY677 CEP to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	107.852 µL	539.258 µL	1.079 mL	5.393 mL	10.785 mL
5 mM	21.57 µL	107.852 µL	215.703 µL	1.079 mL	2.157 mL
10 mM	10.785 µL	53.926 µL	107.852 µL	539.258 µL	1.079 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	927.2 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Insulin-Like Growth Factor-1 Receptor Targeted Fluorescent Imaging for Gallbladder Cancer in Orthotopic Mouse Models.

Authors:

Choi, Jung Ha and Park, Jeong Youp

Journal:

Gut and liver (2021)

Stability Enhancement of a Dimeric HER2-Specific Affibody Molecule through Sortase A-Catalyzed Head-to-Tail Cyclization.

Authors:

Westerlund, Kristina and Myrhammar, Anders and Tano, Hanna and Gestin, Maxime and Karlström, Amelie Eriksson

Journal:

Molecules (Basel, Switzerland) (2021)

Synthesis of Fluorescently Labeled Antibodies Using Non-Canonical Amino Acids in Eukaryotic Cell-Free Systems.

Authors:

Stech, Marlitt and Rakotoarinoro, Nathanaël and Teichmann, Tamara and Zemella, Anne and Thoring, Lena and Kubick, Stefan

Journal:

Methods in molecular biology (Clifton, N.J.) (2021): 175-190

Novel EBV LMP1 C-terminal domain binding affibody molecules as potential agents for in vivo molecular imaging diagnosis of nasopharyngeal carcinoma.

Authors:

Kamara, Saidu and Guo, Yanru and Mao, Shanshan and Ye, Xiaoxian and Li, Qingfeng and Zheng, Maolin and Zhu, Jinshun and Zhang, Jing and Du, Wangqi and Chen, Jun and Zhu, Shanli and Zhang, Lifang

Journal:

Applied microbiology and biotechnology (2021): 7283-7293

Simple methodology to visualize whole-brain microvasculature in three dimensions.

Authors:

Khouri, Katiana and Xie, Danny F and Crouzet, Christian and Bahani, Adrian W and Cribbs, David H and Fisher, Mark J and Choi, Bernard

Journal:

Neurophotonics (2021): 025004

Physical properties

Molecular weight	927.20
Solvent	MeCN

Spectral properties

Excitation (nm)	682
Emission (nm)	702

Usage and storage

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on August 29, 2026