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EDANS C5 maleimide

EDANS is one of the most popular donors for developing FRET-based nucleic acid probes (such as Molecular Beacons) and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive. EDANS C5 maleimide is a thiol-reactive EDANS derivative. It is water-soluble, and has the same spectral properties with EDANS.

Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)Correction Factor (260 nm)
EDANS C2 maleimide3364555900-0.107-
XFD488 C5 Maleimide *Same Structure to Alexa Fluor™ 488 C5 Maleimide*499520710000.9210.110.30
XFD350 C5 Maleimide *Same Structure to Alexa Fluor™ 350 C5 Maleimide*34344119000-0.190.25
XFD532 C5 Maleimide *Same Structure to Alexa Fluor™ 532 C5 Maleimide*534553810000.6110.090.24
XFD594 C5 Maleimide *Same Structure to Alexa Fluor™ 594 C5 Maleimide*590618900000.6610.560.43
XFD405 C5 maleimide40142135,000-0.700.23
XFD430 C5 maleimide43254015,000-0.28-
XFD546 C5 maleimide5615721120000.7910.120.21
XFD568 C5 maleimide579603913000.6910.460.45

References

View all 3 references: Citation Explorer
Synthesis and evaluation of fluorogenic triglycerides as lipase assay substrates.
Authors: Andersen, Rokhsana J and Brask, Jesper
Journal: Chemistry and physics of lipids (2016): 72-9
FRET-Protease-Coupled Peptidyl-Prolyl cis-trans Isomerase Assay: New Internally Quenched Fluorogenic Substrates for High-Throughput Screening.
Authors: Caporale, Andrea and Mascanzoni, Fabiola and Farina, Biancamaria and Sturlese, Mattia and Di Sorbo, Gianluigi and Fattorusso, Roberto and Ruvo, Menotti and Doti, Nunzianna
Journal: Journal of biomolecular screening (2016): 701-12
Characterization and inhibition of SARS-coronavirus main protease.
Authors: Liang, Po-Huang
Journal: Current topics in medicinal chemistry (2006): 361-76
Page updated on October 8, 2024

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Catalog Number619
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Physical properties

Molecular weight

417.44

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.107

Extinction coefficient (cm -1 M -1)

5900

Excitation (nm)

336

Emission (nm)

455

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501