EDANS C5 maleimide

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Physical properties

Molecular weight	417.44
Solvent	DMSO

Spectral properties

Correction Factor (280 nm)	0.107
Extinction coefficient (cm ^-1 M ^-1)	5900
Excitation (nm)	336
Emission (nm)	455

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure
UNSPSC	12171501

Overview SDS Protocol

Molecular weight

417.44

Correction Factor (280 nm)

0.107

Extinction coefficient (cm ^-1 M ^-1)

5900

Excitation (nm)

336

Emission (nm)

455

EDANS is one of the most popular donors for developing FRET-based nucleic acid probes (such as Molecular Beacons) and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive. EDANS C5 maleimide is a thiol-reactive EDANS derivative. It is water-soluble, and has the same spectral properties with EDANS.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EDANS C5 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	239.555 µL	1.198 mL	2.396 mL	11.978 mL	23.956 mL
5 mM	47.911 µL	239.555 µL	479.111 µL	2.396 mL	4.791 mL
10 mM	23.956 µL	119.778 µL	239.555 µL	1.198 mL	2.396 mL

Molarity calculator

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Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
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Spectrum

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Spectral properties

Correction Factor (280 nm)	0.107
Extinction coefficient (cm ^-1 M ^-1)	5900
Excitation (nm)	336
Emission (nm)	455

Product Family

Name	Excitation (nm)	Emission (nm)	Extinction coefficient (cm ^-1 M ^-1)	Quantum yield	Correction Factor (280 nm)	Correction Factor (260 nm)
EDANS C2 maleimide	336	455	5900	-	0.107	-
XFD488 C5 Maleimide Same Structure to Alexa Fluor™ 488 C5 Maleimide	499	520	71000	0.92¹	0.11	0.30
XFD350 C5 Maleimide Same Structure to Alexa Fluor™ 350 C5 Maleimide	343	441	19000	-	0.19	0.25
XFD532 C5 Maleimide Same Structure to Alexa Fluor™ 532 C5 Maleimide	534	553	81000	0.61¹	0.09	0.24
XFD594 C5 Maleimide Same Structure to Alexa Fluor™ 594 C5 Maleimide	590	618	90000	0.66¹	0.56	0.43

Images

Figure 1. Chemical structure for EDANS C5 maleimide.

References

View all 3 references: Citation Explorer

FRET-Protease-Coupled Peptidyl-Prolyl cis-trans Isomerase Assay: New Internally Quenched Fluorogenic Substrates for High-Throughput Screening.
Authors: Caporale, Andrea and Mascanzoni, Fabiola and Farina, Biancamaria and Sturlese, Mattia and Di Sorbo, Gianluigi and Fattorusso, Roberto and Ruvo, Menotti and Doti, Nunzianna
Journal: Journal of biomolecular screening (2016): 701-12

Synthesis and evaluation of fluorogenic triglycerides as lipase assay substrates.
Authors: Andersen, Rokhsana J and Brask, Jesper
Journal: Chemistry and physics of lipids (2016): 72-9

Characterization and inhibition of SARS-coronavirus main protease.
Authors: Liang, Po-Huang
Journal: Current topics in medicinal chemistry (2006): 361-76