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EDANS C5 maleimide

Chemical structure for EDANS C5 maleimide.
Chemical structure for EDANS C5 maleimide.
Ordering information
Price ()
Catalog Number619
Unit Size
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Additional ordering information
Telephone1-408-733-1055
Fax1-408-733-1304
Emailsales@aatbio.com
InternationalSee distributors
ShippingStandard overnight for United States, inquire for international
Physical properties
Molecular weight417.44
SolventDMSO
Spectral properties
Correction Factor (280 nm)0.107
Extinction coefficient (cm -1 M -1)5900
Excitation (nm)336
Emission (nm)455
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure
UNSPSC12171501

OverviewpdfSDSpdfProtocol


Molecular weight
417.44
Correction Factor (280 nm)
0.107
Extinction coefficient (cm -1 M -1)
5900
Excitation (nm)
336
Emission (nm)
455
EDANS is one of the most popular donors for developing FRET-based nucleic acid probes (such as Molecular Beacons) and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive. EDANS C5 maleimide is a thiol-reactive EDANS derivative. It is water-soluble, and has the same spectral properties with EDANS.

Calculators


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EDANS C5 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM239.555 µL1.198 mL2.396 mL11.978 mL23.956 mL
5 mM47.911 µL239.555 µL479.111 µL2.396 mL4.791 mL
10 mM23.956 µL119.778 µL239.555 µL1.198 mL2.396 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)Molecular weightVolume (Calculate)Concentration (Calculate)Moles
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Spectrum


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spectrum

Spectral properties

Correction Factor (280 nm)0.107
Extinction coefficient (cm -1 M -1)5900
Excitation (nm)336
Emission (nm)455

References


View all 3 references: Citation Explorer
FRET-Protease-Coupled Peptidyl-Prolyl cis-trans Isomerase Assay: New Internally Quenched Fluorogenic Substrates for High-Throughput Screening.
Authors: Caporale, Andrea and Mascanzoni, Fabiola and Farina, Biancamaria and Sturlese, Mattia and Di Sorbo, Gianluigi and Fattorusso, Roberto and Ruvo, Menotti and Doti, Nunzianna
Journal: Journal of biomolecular screening (2016): 701-12
Synthesis and evaluation of fluorogenic triglycerides as lipase assay substrates.
Authors: Andersen, Rokhsana J and Brask, Jesper
Journal: Chemistry and physics of lipids (2016): 72-9
Characterization and inhibition of SARS-coronavirus main protease.
Authors: Liang, Po-Huang
Journal: Current topics in medicinal chemistry (2006): 361-76