AAT Bioquest

EDANS C5 maleimide

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Physical properties
Molecular weight417.44
Spectral properties
Correction Factor (280 nm)0.107
Extinction coefficient (cm -1 M -1)5900
Excitation (nm)336
Emission (nm)455
Storage, safety and handling
H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Correction Factor (280 nm)
Extinction coefficient (cm -1 M -1)
Excitation (nm)
Emission (nm)
EDANS is one of the most popular donors for developing FRET-based nucleic acid probes (such as Molecular Beacons) and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive. EDANS C5 maleimide is a thiol-reactive EDANS derivative. It is water-soluble, and has the same spectral properties with EDANS.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EDANS C5 maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM239.555 µL1.198 mL2.396 mL11.978 mL23.956 mL
5 mM47.911 µL239.555 µL479.111 µL2.396 mL4.791 mL
10 mM23.956 µL119.778 µL239.555 µL1.198 mL2.396 mL

Molarity calculator

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Spectral properties

Correction Factor (280 nm)0.107
Extinction coefficient (cm -1 M -1)5900
Excitation (nm)336
Emission (nm)455

Product Family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yieldCorrection Factor (280 nm)Correction Factor (260 nm)
EDANS C2 maleimide3364555900-0.107-
XFD488 C5 Maleimide *Same Structure to Alexa Fluor™ 488 C5 Maleimide*499520710000.9210.110.30
XFD350 C5 Maleimide *Same Structure to Alexa Fluor™ 350 C5 Maleimide*34344119000-0.190.25
XFD532 C5 Maleimide *Same Structure to Alexa Fluor™ 532 C5 Maleimide*534553810000.6110.090.24
XFD594 C5 Maleimide *Same Structure to Alexa Fluor™ 594 C5 Maleimide*590618900000.6610.560.43



View all 3 references: Citation Explorer
Synthesis and evaluation of fluorogenic triglycerides as lipase assay substrates.
Authors: Andersen, Rokhsana J and Brask, Jesper
Journal: Chemistry and physics of lipids (2016): 72-9
FRET-Protease-Coupled Peptidyl-Prolyl cis-trans Isomerase Assay: New Internally Quenched Fluorogenic Substrates for High-Throughput Screening.
Authors: Caporale, Andrea and Mascanzoni, Fabiola and Farina, Biancamaria and Sturlese, Mattia and Di Sorbo, Gianluigi and Fattorusso, Roberto and Ruvo, Menotti and Doti, Nunzianna
Journal: Journal of biomolecular screening (2016): 701-12
Characterization and inhibition of SARS-coronavirus main protease.
Authors: Liang, Po-Huang
Journal: Current topics in medicinal chemistry (2006): 361-76