EDANS sodium salt [5-((2-Aminoethyl)aminonaphthalene-1-sulfonic acid, sodium salt] *CAS 100900-07-0*
EDANS sodium salt is much more water-soluble than EDANS acid. It is one of the most popular donors for developing FRET-based nucleic acid probes (such as Molecular Beacons) and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive.
Calculators
Common stock solution preparation
Table 1. Volume of DMSO needed to reconstitute specific mass of EDANS sodium salt [5-((2-Aminoethyl)aminonaphthalene-1-sulfonic acid, sodium salt] *CAS 100900-07-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.
0.1 mg | 0.5 mg | 1 mg | 5 mg | 10 mg | |
1 mM | 346.861 µL | 1.734 mL | 3.469 mL | 17.343 mL | 34.686 mL |
5 mM | 69.372 µL | 346.861 µL | 693.722 µL | 3.469 mL | 6.937 mL |
10 mM | 34.686 µL | 173.43 µL | 346.861 µL | 1.734 mL | 3.469 mL |
Molarity calculator
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Spectrum
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Product family
Name | Excitation (nm) | Emission (nm) | Extinction coefficient (cm -1 M -1) | Quantum yield |
Resorufin, sodium salt *CAS 34994-50-8* | 571 | 584 | 650001 | 0.751 |
Resazurin, sodium salt *CAS 62758-13-8* | 571 | 584 | - | - |
References
View all 54 references: Citation Explorer
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Authors: Ghosh JG, Houck SA, Doneanu CE, Clark JI.
Journal: J Mol Biol (2006): 364
Authors: Ghosh JG, Houck SA, Doneanu CE, Clark JI.
Journal: J Mol Biol (2006): 364
Recombinant expression and partial characterization of an active soluble histo-aspartic protease from Plasmodium falciparum
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88
Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626
A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110
Characterization and inhibition of SARS-coronavirus main protease
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361
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