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EDANS sodium salt [5-((2-Aminoethyl)aminonaphthalene-1-sulfonic acid, sodium salt] *CAS 100900-07-0*

EDANS sodium salt is much more water-soluble than EDANS acid. It is one of the most popular donors for developing FRET-based nucleic acid probes (such as Molecular Beacons) and protease substrates. EDANS is often paired with DABCYL or DABSYL in FRET-based probes. Its fluorescence is environment-sensitive.

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EDANS sodium salt [5-((2-Aminoethyl)aminonaphthalene-1-sulfonic acid, sodium salt] *CAS 100900-07-0* to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM346.861 µL1.734 mL3.469 mL17.343 mL34.686 mL
5 mM69.372 µL346.861 µL693.722 µL3.469 mL6.937 mL
10 mM34.686 µL173.43 µL346.861 µL1.734 mL3.469 mL

Molarity calculator

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Spectrum

Product family

NameExcitation (nm)Emission (nm)Extinction coefficient (cm -1 M -1)Quantum yield
Resorufin, sodium salt *CAS 34994-50-8*5715846500010.751
Resazurin, sodium salt *CAS 62758-13-8*571584--

References

View all 54 references: Citation Explorer
The beta4-beta8 Groove Is an ATP-interactive Site in the alpha Crystallin Core Domain of the Small Heat Shock Protein, Human alphaB Crystallin
Authors: Ghosh JG, Houck SA, Doneanu CE, Clark JI.
Journal: J Mol Biol (2006): 364
Recombinant expression and partial characterization of an active soluble histo-aspartic protease from Plasmodium falciparum
Authors: Xiao H, Sinkovits AF, Bryksa BC, Ogawa M, Yada RY.
Journal: Protein Expr Purif (2006): 88
Internally quenched peptides for the study of lysostaphin: An antimicrobial protease that kills Staphylococcus aureus
Authors: Warfield R, Bardelang P, Saunders H, Chan WC, Penfold C, James R, Thomas NR.
Journal: Org Biomol Chem (2006): 3626
A quenched fluorescent dipeptide for assaying dispase- and thermolysin-like proteases
Authors: Weimer S, Oertel K, Fuchsbauer HL.
Journal: Anal Biochem (2006): 110
Characterization and inhibition of SARS-coronavirus main protease
Authors: Liang PH., undefined
Journal: Curr Top Med Chem (2006): 361
Page updated on October 11, 2024

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Physical properties

Molecular weight

288.30

Solvent

DMSO

Spectral properties

Correction Factor (280 nm)

0.107

Extinction coefficient (cm -1 M -1)

5900

Excitation (nm)

336

Emission (nm)

455

Storage, safety and handling

H-phraseH303, H313, H333
Hazard symbolXN
Intended useResearch Use Only (RUO)
R-phraseR20, R21, R22

Storage

Freeze (< -15 °C); Minimize light exposure
UNSPSC12171501

CAS

100900-07-0
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