EDTA, AM ester

Ethylenediaminetetraacetic acid (EDTA) might be the most popular metal ion chelator used for both industrial and medical purposes. It is widely used to chelate transitional metal ions, e.g., nickel, cobalt, ferric, ferrous, mercury, lead and other metal ions such as calcium and magnesium. After being bound by EDTA into a metal complex, metal ions generally exhibit diminished reactivity. EDTA is not permeable to live cells. EDTA, AM ester is the cell-permeant derivative of EDTA. Live cells are passively loaded with EDTA, AM by incubation with the cell membrane-permeant EDTA AM ester. Once internalized, cytosolic esterases cleave the AM esters to liberate the active tetra-carboxylate ligand EDTA that sequesters intracellular metal ions indiscriminately. For example, it can be used for sequestering cytosolic Ca2+ for investigations of the role of cytosolic calcium ion, or it can be used to sequester heavy metal contaminants or to inhibit metalloenzymes and metalloproteases that require divalent cations for activity.

Protocol

COA

Catalog	Size	Price	Quantity
19010	10x50 ug	Price
19011	10 mg	Price

Calculators

Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EDTA, AM ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	172.265 µL	861.326 µL	1.723 mL	8.613 mL	17.227 mL
5 mM	34.453 µL	172.265 µL	344.531 µL	1.723 mL	3.445 mL
10 mM	17.227 µL	86.133 µL	172.265 µL	861.326 µL	1.723 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	580.5 g/mol	=	mL	x	mM	=	mmol

Citations

View all 8 citations: Citation Explorer

Limiting Mrs2-dependent mitochondrial Mg2+ uptake induces metabolic programming in prolonged dietary stress

Authors:

Madaris, Travis R and Venkatesan, Manigandan and Maity, Soumya and Stein, Miriam C and Vishnu, Neelanjan and Venkateswaran, Mridula K and Davis, James G and Ramachandran, Karthik and Uthayabalan, Sukanthathulse and Allen, Cristel and others,

Journal:

Cell Reports (2023)

Effect of EDTA root conditioning on the outcome of coronally advanced flap with connective tissue graft: a systematic review and meta-analysis

Authors:

Barootchi, S., Tavelli, L., Ravida, A., Wang, C. W., Wang, H. L.

Journal:

Clin Oral Investig (2018): 2727-2741

Long-term risks of kidney living donation: review and position paper by the ERA-EDTA DESCARTES working group

Authors:

Maggiore, U., Budde, K., Heemann, U., Hilbr and s, L., Oberbauer, R., Oniscu, G. C., Pascual, J., Schwartz Sorensen, S., Viklicky, O., Abramowicz, D., Era-Edta Descartes working group

Journal:

Nephrol Dial Transplant (2017): 216-223

Expanding access to HIV viral load testing: a systematic review of RNA stability in EDTA tubes and PPT beyond current time and temperature thresholds

Authors:

Bonner, K., Siemieniuk, R. A., Boozary, A., Roberts, T., Fajardo, E., Cohn, J.

Journal:

PLoS One (2014): e113813

EDTA chelation reappraisal following new clinical trials and regular use in millions of patients: review of preliminary findings and risk/benefit assessment

Authors:

Born, T., Kontoghiorghe, C. N., Spyrou, A., Kolnagou, A., Kontoghiorghes, G. J.

Journal:

Toxicol Mech Methods (2013): 11-7

Physical properties

Molecular weight	580.50
Solvent	DMSO

Storage, safety and handling

Intended use	Research Use Only (RUO)
Storage	Freeze (< -15 °C); Minimize light exposure
CAS	162303-59-5

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Page updated on October 13, 2025