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EDTA, AM ester

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Physical properties
Molecular weight580.50
Storage, safety and handling
Intended useResearch Use Only (RUO)
StorageFreeze (< -15 °C); Minimize light exposure


Molecular weight
Ethylenediaminetetraacetic acid (EDTA) might be the most popular metal ion chelator used for both industrial and medical purposes. It is widely used to chelate transitional metal ions, e.g., nickel, cobalt, ferric, ferrous, mercury, lead and other metal ions such as calcium and magnesium. After being bound by EDTA into a metal complex, metal ions generally exhibit diminished reactivity. EDTA is not permeable to live cells. EDTA, AM ester is the cell-permeant derivative of EDTA. Live cells are passively loaded with EDTA, AM by incubation with the cell membrane-permeant EDTA AM ester. Once internalized, cytosolic esterases cleave the AM esters to liberate the active tetra-carboxylate ligand EDTA that sequesters intracellular metal ions indiscriminately. For example, it can be used for sequestering cytosolic Ca2+ for investigations of the role of cytosolic calcium ion, or it can be used to sequester heavy metal contaminants or to inhibit metalloenzymes and metalloproteases that require divalent cations for activity.


Common stock solution preparation

Table 1. Volume of DMSO needed to reconstitute specific mass of EDTA, AM ester to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

0.1 mg0.5 mg1 mg5 mg10 mg
1 mM172.265 µL861.326 µL1.723 mL8.613 mL17.227 mL
5 mM34.453 µL172.265 µL344.531 µL1.723 mL3.445 mL
10 mM17.227 µL86.133 µL172.265 µL861.326 µL1.723 mL

Molarity calculator

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View all 8 citations: Citation Explorer
Limiting Mrs2-dependent mitochondrial Mg2+ uptake induces metabolic programming in prolonged dietary stress
Authors: Madaris, Travis R and Venkatesan, Manigandan and Maity, Soumya and Stein, Miriam C and Vishnu, Neelanjan and Venkateswaran, Mridula K and Davis, James G and Ramachandran, Karthik and Uthayabalan, Sukanthathulse and Allen, Cristel and others,
Journal: Cell Reports (2023)
Effect of EDTA root conditioning on the outcome of coronally advanced flap with connective tissue graft: a systematic review and meta-analysis
Authors: Barootchi, S., Tavelli, L., Ravida, A., Wang, C. W., Wang, H. L.
Journal: Clin Oral Investig (2018): 2727-2741
Long-term risks of kidney living donation: review and position paper by the ERA-EDTA DESCARTES working group
Authors: Maggiore, U., Budde, K., Heemann, U., Hilbr and s, L., Oberbauer, R., Oniscu, G. C., Pascual, J., Schwartz Sorensen, S., Viklicky, O., Abramowicz, D., Era-Edta Descartes working group
Journal: Nephrol Dial Transplant (2017): 216-223
Expanding access to HIV viral load testing: a systematic review of RNA stability in EDTA tubes and PPT beyond current time and temperature thresholds
Authors: Bonner, K., Siemieniuk, R. A., Boozary, A., Roberts, T., Fajardo, E., Cohn, J.
Journal: PLoS One (2014): e113813
EDTA chelation reappraisal following new clinical trials and regular use in millions of patients: review of preliminary findings and risk/benefit assessment
Authors: Born, T., Kontoghiorghe, C. N., Spyrou, A., Kolnagou, A., Kontoghiorghes, G. J.
Journal: Toxicol Mech Methods (2013): 11-7
EDTA chelation therapy for cardiovascular disease: a systematic review
Authors: Seely, D. M., Wu, P., Mills, E. J.
Journal: BMC Cardiovasc Disord (2005): 32
EDTA-dependent pseudothrombocytopenia: a clinical study of 18 patients and a review of the literature
Authors: Berkman, N., Michaeli, Y., Or, R., Eldor, A.
Journal: Am J Hematol (1991): 195-201
EDTA, the traditional anticoagulant of haematology: with increased automation is it time for a review?
Authors: Reardon, D. M., Warner, B., Trowbridge, E. A.
Journal: Med Lab Sci (1991): 72-5