Name: EDTA Maleimide
Brand: AAT Bioquest
SKU: 65006
Price: 301 USD
Availability: InStock

EDTA Maleimide

EDTA maleimide is a thiol-reactive metal chelator that selectively conjugates to cysteine residues, enabling site-specific introduction of metal-binding properties into proteins and peptides.

Thiol-selective Chemistry: Maleimide group rapidly reacts with cysteine residues at pH 6.5–7.5
Site-specific Conjugation: Targets free sulfhydryls for precise protein modification
Metal Ion Affinity: Retains EDTA's strong chelation of Ca²⁺, Mg²⁺, and heavy metals
Protein Purification Applications: Creates metal-affinity tags for IMAC chromatography

Datasheet

SDS

COA

Catalog	Size	Price	Quantity
65006	5 mg	Price

Overview

EDTA maleimide is an excellent building block to develop site-specific ethylenediaminetetraacetic acid (EDTA) probes for variety of biomedical applications. It readily reacts with a thiol compound under extremely mild conditions. EDTA is the most commonly used chelator for complexing transitional metal ions. EDTA chelation therapy uses the chelating agent, EDTA, to remove heavy metals and minerals from the body. It involves injecting EDTA into the bloodstream, where it binds to these substances, allowing them to be excreted through urine. EDTA is approved for treating lead poisoning, but it's also used in other conditions, including coronary artery disease and arthritis.

Calculators

Common stock solution preparation

Table 1. Volume of DMF needed to reconstitute specific mass of EDTA Maleimide to given concentration. Note that volume is only for preparing stock solution. Refer to sample experimental protocol for appropriate experimental/physiological buffers.

	0.1 mg	0.5 mg	1 mg	5 mg	10 mg
1 mM	144.789 µL	723.945 µL	1.448 mL	7.239 mL	14.479 mL
5 mM	28.958 µL	144.789 µL	289.578 µL	1.448 mL	2.896 mL
10 mM	14.479 µL	72.395 µL	144.789 µL	723.945 µL	1.448 mL

Molarity calculator

Enter any two values (mass, volume, concentration) to calculate the third.

Mass (Calculate)		Molecular weight		Volume (Calculate)		Concentration (Calculate)		Moles
mg	÷	690.66 g/mol	=	mL	x	mM	=	mmol

References

View all 50 references: Citation Explorer

Season-based phytoremediation potential of brown mustard for lead decontamination: effect of EDTA chelation and antioxidant enzyme activity.

Authors:

Chen, Deqiang and Ibrahim, Muhammad and Soroma, Mara and Danjaji, Hafsat Alhassan and Jibo, Adam Umar and Yang, Ying

Journal:

International journal of phytoremediation (2025): 1-13

Combined Topography-Guided Trans-Epithelial PRK and PTK for Treatment of Calcific Band Keratopathy Unresponsive to EDTA Chelation Therapy.

Authors:

Passidomo, Fedele and Addabbo, Giuseppe and Pignatelli, Francesco and Niro, Alfredo and Buonamassa, Rosa

Journal:

International medical case reports journal (2025): 187-194

Severe Descemetocele after EDTA Chelation for Band Keratopathy.

Authors:

Montolío-Marzo, Santiago and Rodríguez-Iranzo, Guillermo and Peris-Martínez, Cristina

Journal:

Ophthalmology (2024): 395

Excimer laser-assisted phototherapeutic keratectomies combined to EDTA chelation for the treatment of calcific band keratopathy.

Authors:

Spadea, Leopoldo and Giannico, Maria Ilaria and Iannaccone, Andrea and Pistella, Santino

Journal:

European journal of ophthalmology (2022): NP42-NP46

SARS-CoV-2 attachment to host cells is possibly mediated via RGD-integrin interaction in a calcium-dependent manner and suggests pulmonary EDTA chelation therapy as a novel treatment for COVID 19.

Authors:

Dakal, Tikam Chand

Journal:

Immunobiology (2021): 152021

Physical properties

Molecular weight	690.66
Solvent	DMF

Storage, safety and handling

H-phrase	H303, H313, H333
Hazard symbol	XN
Intended use	Research Use Only (RUO)
R-phrase	R20, R21, R22
Storage	Freeze (< -15 °C); Minimize light exposure

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Page updated on May 9, 2026