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EGTA AM *10 mM DMSO solution*
EGTA AM is cell-permeable version of EGTA (ethylene glycol tetraacetic acid), a cell-permeable calcium chelator. This EGTA derivative is used for adjusting calcium concentrations in cells and tissues. EGTA is an aminopolycarboxylic acid, a chelating agent. Compared to EDTA, EGTA has a lower affinity for magnesium, making it more selective for calcium ions. It is useful in buffer solutions that resemble the environment in living cells where calcium ions are usually at least a thousand fold less concentrated than magnesium. The pKa for binding of calcium ions by tetrabasic EGTA is 11.00, but the protonated forms do not significantly contribute to binding, so at pH 7, the apparent pKa becomes 6.91.
Citations
View all 4 citations: Citation Explorer
Dual ablation of the RyR2-Ser2808 and RyR2-Ser2814 sites increases propensity for pro-arrhythmic spontaneous Ca2+ releases
Authors: Janicek, Radoslav and Camors, Emmanuel M and Potenza, Duilio M and Fernandez-Tenorio, Miguel and Zhao, Yanting and Dooge, Holly C and Loaiza, Randall and Alvarado, Francisco J and Egger, Marcel and Valdivia, Hector H and others,
Journal: The Journal of Physiology (2024)
Authors: Janicek, Radoslav and Camors, Emmanuel M and Potenza, Duilio M and Fernandez-Tenorio, Miguel and Zhao, Yanting and Dooge, Holly C and Loaiza, Randall and Alvarado, Francisco J and Egger, Marcel and Valdivia, Hector H and others,
Journal: The Journal of Physiology (2024)
Distortion of the Actin A-Triad Results in Contractile Disinhibition and Cardiomyopathy
Authors: Viswanathan, Meera C and Schmidt, William and Rynkiewicz, Michael J and Agarwal, Karuna and Gao, Jian and Katz, Joseph and Lehman, William and Cammarato, Anthony
Journal: Cell Reports (2017): 2612--2625
Authors: Viswanathan, Meera C and Schmidt, William and Rynkiewicz, Michael J and Agarwal, Karuna and Gao, Jian and Katz, Joseph and Lehman, William and Cammarato, Anthony
Journal: Cell Reports (2017): 2612--2625
Routes of Ca2+ shuttling during Ca2+ oscillations: Focus on the role of mitochondrial Ca2+ handling and cytosolic Ca2+ buffers
Authors: Pecze, L{\'a}szl{\'o} and Blum, Walter and Schwaller, Beat
Journal: Journal of Biological Chemistry (2015): 28214--28230
Authors: Pecze, L{\'a}szl{\'o} and Blum, Walter and Schwaller, Beat
Journal: Journal of Biological Chemistry (2015): 28214--28230
Routes of Ca2+ Shuttling during Ca2+ Oscillations FOCUS ON THE ROLE OF MITOCHONDRIAL Ca2+ HANDLING AND CYTOSOLIC Ca2+ BUFFERS
Authors: Pecze, László and Blum, Walter and Schwaller, Beat
Journal: Journal of Biological Chemistry (2015): 28214--28230
Authors: Pecze, László and Blum, Walter and Schwaller, Beat
Journal: Journal of Biological Chemistry (2015): 28214--28230
References
View all 77 references: Citation Explorer
Tuning caged calcium: photolabile analogues of EGTA with improved optical and chelation properties
Authors: Ellis-Davies GC, Barsotti RJ.
Journal: Cell Calcium (2006): 75
Authors: Ellis-Davies GC, Barsotti RJ.
Journal: Cell Calcium (2006): 75
Catalytic superoxide scavenging by metal complexes of the calcium chelator EGTA and contrast agent EHPG
Authors: Fisher AE, Hague TA, Clarke CL, Naughton DP.
Journal: Biochem Biophys Res Commun (2004): 163
Authors: Fisher AE, Hague TA, Clarke CL, Naughton DP.
Journal: Biochem Biophys Res Commun (2004): 163
Voltage-activated calcium signals in myotubes loaded with high concentrations of EGTA
Authors: Schuhmeier RP, Dietze B, Ursu D, Lehmann-Horn F, Melzer W.
Journal: Biophys J (2003): 1065
Authors: Schuhmeier RP, Dietze B, Ursu D, Lehmann-Horn F, Melzer W.
Journal: Biophys J (2003): 1065
Role of calcium in nitric oxide-induced cytotoxicity: EGTA protects mouse oligodendrocytes
Authors: Boullerne AI, Nedelkoska L, Benjamins JA.
Journal: J Neurosci Res (2001): 124
Authors: Boullerne AI, Nedelkoska L, Benjamins JA.
Journal: J Neurosci Res (2001): 124
L-type calcium channel blockers and EGTA enhance superoxide production in cardiac fibroblasts
Authors: Shivakumar K, Kumaran C.
Journal: J Mol Cell Cardiol (2001): 373
Authors: Shivakumar K, Kumaran C.
Journal: J Mol Cell Cardiol (2001): 373
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